Somatic Embryogenesis from Corolla Tubes of Interspecific Amphiploids between Cultivated Sunflower (Helianthus annuus L.) and Its Wild Species

Fu, Xinliang; Qi, Lili; Hulke, Brent S.; Seiler, Gerald J.; Jan, Chao‐Chien

doi:10.1515/helia-2017-0006

Cited by 3 publications

(1 citation statement)

References 48 publications

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“…This showed that the somatic embryo development process is asynchronous. Similar results have been found in Hibiscus sabdariffa (Konar et al 2018), Akebia trifoliata (Zou et al 2019), Helianthus annuus L. (Fu et al 2017), and Capsicum baccatum (Venkataiah et al 2016). Finally, when the cotyledonary stage embryos were sub-cultured in MS medium containing 1 mgL -1 BAP at 12 WAC, they were converted into complete plantlets (Figure 1.G).…”

Section: Induction Of Embryogenic Calli Developmentsupporting

confidence: 84%

Development and regeneration of somatic embryos from leaves-derived calli of Coffea liberica

et al. 2020

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Abstract. Ardiyani F, Utami ESW, Purnobasuki H, Paramita SA. 2020. Development and regeneration of somatic embryos from leaves-derived calli of Coffea liberica. Biodiversitas 21: 5829-5834. Coffea liberica is an important and potentially commercial plant with a high economic value from the Coffea genus. Therefore, the availability of planting material is needed to increase productivity and ensure the sustainability of its farming. Somatic embryogenesis is a powerful propagation method used to produce clonal plants from limited genetic material. In the present research, we have shown that C. liberica could be successfully regenerated in vitro via somatic embryogenesis from leaves derived embryogenic callus. These calli were cultured on Murashige Skoog (MS) medium added with 1 mgL-1 BAP or in combination with 2.4 D (0.5, 1.0, 1.5 and 2 mgL-1) for embryo development induction. Furthermore, the medium containing only BAP was best for embryo development induction after culturing for 12 weeks, with the highest number of cotyledonary stage embryos (17.8%) and producing a total of embryo (20.2). Following cotyledonary stage embryo were cultured on new MS medium containing 0.5 mgL-1 BAP, 0.5 mgL-1 IAA, 0.5 mgL-1 NAA only, and 0.5 mgL-1 BAP in combination with 0.5 mgL-1 IAA or 0.5 mgL-1 NAA. Interestingly, the results showed that cotyledonary stage embryos were converted into complete plants at all treatment, but the MS medium containing 0.5 mgL-1 BAP was found to be the most effective in promoting regeneration with 2.6 leaves per-plantlet and height of 5.2 mm. Based morphological analysis confirm that the development of somatic embryo from leaves-derived calli of Coffea liberica started with the formation of embryo globular, heart, torpedo, cotyledonary stages, and finally conversion of cotyledonary embryo into complete plant.

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Section: Induction Of Embryogenic Calli Developmentsupporting

confidence: 84%

Development and regeneration of somatic embryos from leaves-derived calli of Coffea liberica

et al. 2020

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Breeding Strategies for Sunflower (Helianthus annuus L.) Genetic Improvement

Rauf

2019

Advances in Plant Breeding Strategies: Industrial and Food Crops

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Chao-Chien Jan: Thirty-five Years of Dedicated Research Utilizing Wild Sunflower Crop Relatives for Sunflower Improvement

Seiler

2018

Helia

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Dr Chao-Chien Jan, Research Geneticist with the USDA-Agricultural Research Service (USDA-ARS), Red River Valley Agricultural Research Center, Northern Crop Science Laboratory, Sunflower and Plant Biology Research Unit, Fargo, ND retired January, 2017 after 35 years of dedicated service. He began his research career in 1974 after receiving his Ph.D. in genetics from the University of California, Davis, CA, working with wheat. He was a postdoctoral Research Biologist at the Cancer Research Institute, University of California, San Francisco in 1975. From 1976 to 1981 he was a postdoctoral Research Agronomist, Department of Agronomy and Range Science, UC, Davis, CA. working on wheat. In 1981, Dr Jan joined the U.S. Department of Agriculture, Agricultural Research Service (USDA-ARS) as a Research Geneticist at the Rice and Oilseed Unit at Davis, CA with an emphasis on cytogenetics, working on sunflower (Helianthus annuus) crop wild relatives (CWR) for the improvement of the sunflower crop. The sunflower program at Davis, CA was closed in 1984, and he was transferred to the USDA-ARS Sunflower Unit in Fargo, ND where he spent the rest of his career working on sunflower CWR. Dr Jan’ research contributed significantly to the ability to utilize the genetic diversity of the 53 species of wild sunflowers, especially in the areas of germination, use of embryo culture and chromosome doubling to overcome embryo abortion and fertility problems. His pioneering research in interspecific hybridization, cytoplasmic male sterility and fertility restoration, cytogenetic stocks, disease resistance and mutation opened doors to genetic diversity never before available for utilization by the sunflower industry. This led to his global stature with invitations to serve as a visiting scientist and fellowships in Australia, Serbia, Spain, Romania, and China. He has hosted over 20 scientists from 15 countries, as well as countless students during his career. Due to his stature, he has been invited to present several invited plenary talks, both national and international, and in 2012 he was presented the prestigious Pustovoit Award, the highest award in the sunflower industry given by the International Sunflower Association for his contribution to sunflower science and technology.

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Somatic Embryogenesis from Corolla Tubes of Interspecific Amphiploids between Cultivated Sunflower (Helianthus annuus L.) and Its Wild Species

Abstract: Somatic embryogenesis in vitro provides an efficient means of plant multiplication, facilitating sunflower improvement and germplasm innovation. In the present study, using interspecific amphiploids (2n=4x=68) between cultivated sunflower and wild perennial

Cited by 3 publications

References 48 publications

Development and regeneration of somatic embryos from leaves-derived calli of Coffea liberica

Development and regeneration of somatic embryos from leaves-derived calli of Coffea liberica

Breeding Strategies for Sunflower (Helianthus annuus L.) Genetic Improvement

Chao-Chien Jan: Thirty-five Years of Dedicated Research Utilizing Wild Sunflower Crop Relatives for Sunflower Improvement

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