Somatic embryogenesis and shoot regeneration from excised adventitious roots of the rootstock Rosa hybrida L. ‘Moneyway’

Abstract

Plants were regenerated from excised adventitious roots of the rose rootstock 'Moneyway' via a three step procedure: callus induction, induction of somatic embryos and shoot development. Callus was induced on excised roots after incubation for 4 weeks in the dark on SH-medium (Schenk and Hildebrandt) containing 50 μM 2,4-dichlorophenoxyacetic acid. For embryo induction, calluses were transferred to hormone-free SH-medium and incubated for 8 weeks. The use of Gelrite instead of agar during callus induction stimulated somatic embryogenesis (up to 16% of the explants formed organized structures), whereas the presence of 6-benzylaminopurine in this phase inhibited subsequent regeneration. Yellow solid calluses with embryo-like cotyledons or primordia and friable calluses with embryos were selected, and upon incubation in the light shoots developed. Shoot development was faster and more frequent on solid callus than on friable callus (64% and 21% of the calluses finally formed one or more shoots, respectively). Eleven out of thirteen regenerants developed similarly to control shoots. Finally this regeneration method is compared with other systems for somatic embryogenesis and opportunities for the production of transgenic rose rootstocks and rose cultivare are discussed.

Effects of plant growth regulators on the callus of rose leaves

“…The callus treated with NAA alone was pale yellow or white, but some turned brown with dry and loose texture over time. However, after the addition of NAA, KT, ZT, or 6-BA, the callus turned pale green, had a clear texture, and was moist and compact (Theo et al 1996, Liu et al 2017. Tang et al (2017) found that 2,4-D, NAA, KT, ZT, and 6-BA are usually used as the key hormones for plant callus formation.…”

Maturation and Conversion of Somatic Embryos of Three Genetically Diverse Rose Cultivars

“…Several researchers report regeneration of rose plants from callus (de Wit et al, 1990;Hsia and Korban, 1996;Kintzios et al, 1999;Marchant et al, 1996;Matthews et al, 1991;Murali et al, 1996;Noriega and Sondahl, 1991;Valles and Boxus, 1987;van der Salm et al, 1996). The methods are specific to only one or two cultivars.…”