Somatic embryogenesis and plant regeneration from cultured mature and immature embryos of Paspalum notatum (gramineae)

Bovo, Oscar A.; Mroginski, L. A.

doi:10.1016/0168-9452(89)90068-x

Cited by 19 publications

(6 citation statements)

References 23 publications

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“…The in vitro culture and plant transformation protocols used here were adapted from previous works published by Bovo and Mroginski (1989), Grando et al (2002), Smith et al (2002), and Gondo et al (2005). Success rate in the selection of putative transformed plants is mainly dependent upon the dose rate of selective agents.…”

Section: Discussionmentioning

confidence: 99%

“…The mature embryo-based regeneration protocol was based on protocols reported by Bovo and Mroginski (1989) and Grando et al (2002), with modifications. Sterilized Q4117 seeds were placed in sterile distilled water for a period of 1-24 h to soften the surface and facilitate embryo isolation.…”

Section: Methodsmentioning

confidence: 99%

“…Bovo and Mroginski (1989) regenerated plants from tetraploid mature embryos. Marousky and West (1990) used mature caryopses as explants, whereas Shatters et al (1994) employed leaf-stem cross-sections to initiate the plant regeneration process.…”

Section: Introductionmentioning

confidence: 99%

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Development of a modified transformation platform for apomixis candidate genes research in Paspalum notatum (bahiagrass)

Mancini

Woitovich

Permingeat

et al. 2014

In Vitro Cell.Dev.Biol.-Plant

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Development of a modified transformation platform for apomixis candidate genes research in Paspalum notatum (bahiagrass)

Mancini

Woitovich

Permingeat

et al. 2014

In Vitro Cell.Dev.Biol.-Plant

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“…Most of the tissue culture research with forage grasses has been conducted in temperate species. Efficient plant regeneration systems of warm-season grasses have been developed via somatic embryogenesis (Bovo & Mroginski 1989;Akashi & Adachi 1991, 1992Akashi et al 1993;Gondo et al 2007). Plant regeneration from in vitro cultures of ruzigrass has been demonstrated previously via somatic embryogenesis (Tohme et al 1996).…”

Section: Introductionmentioning

confidence: 99%

“…Most of the tissue culture research with forage grasses has been conducted in temperate species. Efficient plant regeneration systems of warm‐season grasses have been developed via somatic embryogenesis (Bovo & Mroginski 1989; Akashi & Adachi 1991, 1992; Akashi et al . 1993; Gondo et al .…”

Section: Introductionmentioning

confidence: 99%

Multiple shoot formation, somatic embryogenesis and plant regeneration from seed‐derived shoot apical meristems in ruzigrass (Brachiaria ruziziensis)

et al. 2009

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We have established a plant regeneration system via multiple shoot formation, or somatic embryogenesis from seed‐derived shoot apical meristems of ruzigrass (Brachiaria ruziziensis). Shoot apices as initial explants were isolated from aseptically germinated seedlings, and cultured in vitro. The most effective treatments for multiple shoots and embryogenic calli formation were 0.5 mg L−1 2,4‐dichlorophnoxyacetic acid (2,4‐D) plus 2.0 mg L−1 6‐benzylaminopurine (BAP) and 4.0 mg L−1 2,4‐D plus 0.2 mg L−1 BAP, respectively. Plant regeneration from multiple shoot‐clumps was achieved by culturing on solid MS medium containing 1.0 mg L−1 BAP or 2.0 mg L−1 kinetin plus 2.0 mg L−1 gibberellic acid. Somatic embryos regenerated plantlets on a MS medium without plant hormones. Regenerated shoots were germinated precociously on MS medium containing 0.01 or 0.1 mg L−1 naphthalene acetic acid and 2.0 mg L−1 BAP. All regenerants were successfully grown up to maturity. The plant regeneration via multiple‐shoot clumps and embryogenic calli formation can be used as an alternative regenerable target tissue for genetic transformation using particle bombardment of ruzigrass.

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Bahiagrass

Altpeter

Agharkar

Sandhu

2008

Compendium of Transgenic Crop Plants

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Bahiagrass is a commercially important warm‐season forage and turf grass in the southeastern United States and other subtropical regions. Its popularity among ranchers and homeowners is attributed to persistence under drought, infertility, pest and disease pressures, mowing, and grazing. Bahiagrass has apomictic tetraploid and sexual diploid genotypes, both of which have commercially important cultivars. Development of improved cultivars by traditional breeding is limited by the genetic variability within diploid genotypes and the lack of genetic recombination in apomictic bahiagrass. Genetic engineering of bahiagrass is likely to contribute to the development of improved cultivars. Alternative genetic transformation protocols will be described. Apomictic bahiagrass is a preferred target for genetic transformation due to enhanced uniformity of seed‐derived progeny and transgene containment. Genetic transformation of apomictic cultivar “Argentine” has recently been successfully used to confer herbicide resistance, insect resistance, stress tolerance, and improved turf quality. Genetic transformation of bahiagrass is discussed with regard to crop improvement, risk assessment, and risk management.

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Somatic embryogenesis and plant regeneration from cultured mature and immature embryos of Paspalum notatum (gramineae)

Cited by 19 publications

References 23 publications

Development of a modified transformation platform for apomixis candidate genes research in Paspalum notatum (bahiagrass)

Development of a modified transformation platform for apomixis candidate genes research in Paspalum notatum (bahiagrass)

Multiple shoot formation, somatic embryogenesis and plant regeneration from seed‐derived shoot apical meristems in ruzigrass (Brachiaria ruziziensis)

Bahiagrass

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