Somatic Embryogenesis and Plant Regeneration from Immature Embryos of Tropical Maize (Zea mays L.) Inbred Lines

Somatic Embryogenesis and Plant Regeneration From an Elite Tropical Maize Inbred Line

“…The only factor that impacted the maturation of embryogenic callus was the presence of CuSO 4 in the culture medium (Table 2). These results differ from those found by Ombori et al (2008) and Akoyi et al (2013), which reported that BAP and NAA were effective in the maturation of somatic embryos of maize. Over a period of 42 days, there was a higher percentage of maturation in the treatments where CuSO 4 was present (Table 2).…”

“…The ideal concentration of 2,4-D for the formation of embryogenic calli has fluctuated between 1.5 and 2 mg.L -1 (Anami et al, 2010;Ombori et al, 2008;Frame et al, 2006). Differences in responses to auxin induction of somatic embryogenesis may occur depending upon the species, genotype or explants used (Fehér, 2015).This fact could be evidenced in this study, where the concentration of 2,4-D used to induce Type II callus in the inbred line L3 was approximately five to seven times higher than the one used by others (Anami et al, 2010;Ombori et al, 2008;Frame et al, 2006) to induce the same kind of callus in different maize genotypes.…”

“…Differences in responses to auxin induction of somatic embryogenesis may occur depending upon the species, genotype or explants used (Fehér, 2015).This fact could be evidenced in this study, where the concentration of 2,4-D used to induce Type II callus in the inbred line L3 was approximately five to seven times higher than the one used by others (Anami et al, 2010;Ombori et al, 2008;Frame et al, 2006) to induce the same kind of callus in different maize genotypes. Pulianmackal et al (2014) stated that a complex set of regulatory interactions are involved in somatic embryogenesis.…”

“…Others indicated that 2,4-D is better to induce the formation of Type II callus from maize zygotic embryos (Songstad et al, 1991). In this study, Dicamba was not adequate to (Anami et al, 2010;Ombori et al, 2008;Frame et al, 2006). Differences in responses to auxin induction of somatic embryogenesis may occur depending upon the species, genotype or explants used (Fehér, 2015).This fact could be evidenced in Some studies reported that media supplemented with Dicamba have been successfully used in the formation of Type II callus in various maize genotypes and others grasses (Bohorova et al, 1995;Carvalho et al, 1997;Akoyi et al, 2013).…”

“…During this phase changes in gene expression happens in the cells leading to the accumulation of storage material (Teoh et al, 2013) and acquisition of desiccation tolerance. Maturation of somatic embryos have been reported in the absence of 2,4-D as well as in the presence of others growth regulators such as cytokines (Ombori et al, 2008;Akoyi et al, 2013), or abscisic acid (ABA). Other components may also be used to promote maturation of embryogenic callus, such as high concentration of sucrose, gelling agents and heavy metals (Fehér, 2015;Cho et al, 2014).…”