Somatic embryogenesis and plant regeneration inCoronilla varia L. (crownvetch) long-term tissue cultures

Morphogenic Aspects of Somatic Embryogenesis

“…Halperin and Wetherell [101] first noticed that the maintenance of globular-stage carrot somatic embryos on 2,4-D inhibited their histodifferentiation or led to abnormal development of the apical meristem. Similar observations have been made for crownvetch [161], and the development of soybean somatic embryos was inversely proportional to length of auxin exposure [162]. Up to 50% of carrot somatic embryos induced with cadmium were reported to germinate, while only 15% of somatic embryos induced with 2,4-D were able to do so [19], illustrating the point that exposure to auxins can have a deleterious effect that eventually affects germination capacity.…”

First Ex Situ In Vitro Propagation Protocol of Coronilla viminalis Salisb., An Endangered Fodder Species Adapted to Drought and Salinity

“…Unfortunately, no such propagation protocols have been described to date. In the literature, it is possible only to find some tissue-culture approaches for other family species (i.e., Coronilla varia L.) for which a protocol for somatic embryogenesis was developed [7,8]. In those trials, the auxin IAA was present in all protocols.…”

Somatic Embryogenesis and Synthetic Seed II

“…Plants had been regenerated via organogenesis from 54 different legume species, while only 39 species had been regenerated from somatic embryos . Greinwald and Czygan (1991) Greinwald and Czygan (1991) Rao and Chopra (1989) Weaver and Trigiano (1991) Dhanalakshmi and Lakshmanan (1992) Arcioni and Mariotti (1982); Arcioni et al (1988); Duskova et al (1990) Ramanuja Rao et al (1982) Grant (1984) ; Lazzeri et al (1985Lazzeri et al ( , 1987aLazzeri et al ( , b, 1988; Ranch et al (1985); ; Ghazi et al (1986); Hammatt and Davey (1987); Tetu et al (1987);; Finer and Nagasawa (1988); ; Hepher et al (1988); Komatsuda and Ohyama (1988); Parrott et al ( ,1989; Shoemaker and Hammond (1988); Buchheim et al (1989); Christou and Yang (1989) (1987) Saunders and Bingham (1972); Dos Santos et al (1980...…”