Somatic embryogenesis and plant regeneration of Capsicum baccatum L.

Abstract

A plant regeneration protocol via somatic embryogenesis was achieved in cotyledon and leaf explants of Capsicum baccatum, when cultured on MS medium supplemented with various concentrations of 2,4-dichlorophenoxy acetic acid (2,4-D, 0.5–5.0 mg l−1) in combination with Kinetin (Kn, 0.5 mg l−1) and 3% sucrose. Various stages were observed during the development of somatic embryos, including globular, heart, and torpedo-stages. Torpedo stage embryos were separated from the explants and subcultured on medium supplemented with various concentrations of different plant growth regulators for maturation. Maximum percentage (55%) of somatic embryo germination and plantlet formation was found at 1.0 mg l−1 BA. Finally, about 68% of plantlets were successfully established under field conditions. The regenerated plants were morphologically normal, fertile and able to set viable seeds.

Development and regeneration of somatic embryos from leaves-derived calli of Coffea liberica

“…This showed that the somatic embryo development process is asynchronous. Similar results have been found in Hibiscus sabdariffa (Konar et al 2018), Akebia trifoliata (Zou et al 2019), Helianthus annuus L. (Fu et al 2017), and Capsicum baccatum (Venkataiah et al 2016). Finally, when the cotyledonary stage embryos were sub-cultured in MS medium containing 1 mgL -1 BAP at 12 WAC, they were converted into complete plantlets (Figure 1.G).…”

Somatic Embryogenesis and Plant Regeneration from Cotyledon and Hypocotyl Explants of Fagopyrum esculentum Moench lpls Mutant

“…Previous studies suggested that 2,4-D was necessary for SE in several species such as Capsicum baccatum [21], Tagetes erecta [22], Salicornia brachiate [23] and Cymbopogon winterianus [24]. High indirect somatic embryogenesis from immature embryos of buckwheat also occurred on B 5 medium with 6% sucrose when 5.0 mgL −1 2,4-D and 0.1 mgL −1 KT were supplied at the beginning of the culture period for 5 days, and then transferred to B 5 medium plus 2.2 mgL −1 BAP, 0.17 mgL −1 IAA for germination of somatic embryos [14].…”

Plant Regeneration through Somatic Embryogenesis of Clerodendrum serratum l. and the Respective Study on its Antioxidant Activity and Phenolic Content

“…Under these conditions, embryos continued germinating, shoots and roots started developing within 17-20 days. The resulat is similar with some other reports on different plant like (Bhattacharya ., 2016), (L.) F. Muell (Shekhawat & Manokari, 2016) and (Venkataiah , 2016). Further growth of plantlets was done on the same basal MS medium containing 0.5mg/L BAP.…”