Somatic cell nuclear transfer is associated with altered expression of angiogenic factor systems in bovine placentomes at term

Campos, Danila Barreiro; Papa, Paula de Carvalho; Marques, Jeb; Garbelotti, Fernando; Fátima, Luciana Alves de; Artoni, L. P.; Birgel, Eduardo Harry; Meirelles, Flávio Vieira; Buratini, J.; Leiser, Rudolf; Pfarrer, Christiane

doi:10.4238/vol9-1gmr729

Cited by 20 publications

(15 citation statements)

References 39 publications

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“…cardiovascular system seems to be very sensitive in regard to developmental problems in early gestation, suggesting an altered expression of associated genes [156][157][158][159][160][161]. Vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) genes are regarded as major stimulating factors for such processes and have been proven to be altered at least in term pregnancies of bovines from nuclear transfer [162,163]. Currently, a more comprehensive analysis in bovines derived from various techniques with special reference to the more critical early phases of gestation is under way; first results suggest that the expression of these genes indeed is disturbed (personal observation).…”

Section: Laurasiatheriamentioning

confidence: 99%

Placental Evolution within the Supraordinal Clades of Eutheria with the Perspective of Alternative Animal Models for Human Placentation

Mess

2014

Advances in Biology

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Here a survey of placental evolution is conducted. Placentation is a key factor for the evolution of placental mammals that had evolved an astonishing diversity. As a temporary organ that does not allow easy access, it is still not well understood. The lack of data also is a restriction for better understanding of placental development, structure, and function in the human. Animal models are essential, because experimental access to the human placenta is naturally restricted. However, there is not a single ideal model that is entirely similar to humans. It is particularly important to establish other models than the mouse, which is characterised by a short gestation period and poorly developed neonates that may provide insights only for early human pregnancy. In conclusion, current evolutionary studies have contributed essentially to providing a pool of experimental models for recent and future approaches that may also meet the requirements of a long gestation period and advanced developmental status of the newborn in the human. Suitability and limitations of taxa as alternative animal models are discussed. However, further investigations especially in wildlife taxa should be conducted in order to learn more about the full evolutionary plasticity of the placenta system.

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Section: Laurasiatheriamentioning

confidence: 99%

Placental Evolution within the Supraordinal Clades of Eutheria with the Perspective of Alternative Animal Models for Human Placentation

Mess

2014

Advances in Biology

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“…Real-time PCR analyses were performed using an ABI 7500 Sequence Detection System (PE Applied Biosystems) as described previously (Campos et al 2010) with some modifications to match dog-specific conditions, such as the low concentrations of available cDNA. SLC2A1, HIF1A, VEGFA, FLT1, KDR and FGF2 primers and probes were designed using the Assays-by-Design service from Applied Biosystems (Table 1).…”

Section: Real-time Pcrmentioning

confidence: 99%

Glucose transporter 1 expression accompanies hypoxia sensing in the cyclic canine corpus luteum

et al. 2014

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The canine corpus luteum (CL) functions as a source of progesterone (P 4 ) and 17b-oestradiol (E 2 ); however, the transport of energy substrates to maintain its high hormonal output has not yet been characterised. This study involved the localisation and temporal distribution of the facilitative glucose transporter 1 and the quantification of the corresponding protein (GLUT1) and gene (SLC2A1) expression. Some GLUT1/SLC2A1 regulatory proteins, such as hypoxia-inducible factor 1a (HIF1A) and fibroblast growth factor 2 (FGF2); mRNAs, such as HIF1A, FGF2 and vascular endothelial growth factor A (VEGFA); and VEGFA receptors 1 and 2 (FLT1 and KDR) were also analysed from days 10 to 70 after ovulation. Additionally, plasma P 4 and E 2 levels were assessed via chemiluminescence. Moreover, the canine KDR sequence has been cloned, thereby enabling subsequent semi-quantitative PCR analysis. Our results demonstrate time-dependent variations in the expression profile of SLC2A1 during dioestrus, which were accompanied by highly correlated changes (0.84!r!0.98; P!0.03) in the gene expression of HIF1A, VEGF and FLT1 as well as in P 4 plasma concentrations. FGF2 mRNA correlated with E 2 plasma concentrations (rZ0.61; PZ0.01). Our data reveal that the glucose transporter is regulated throughout the CL lifespan and suggest that CL depends on the sensing of hypoxia and the status of luteal vascularisation. Moreover, time-dependent expression of GLUT1/SLC2A1 may lie underneath increased metabolic and energetic requirements for sustaining P 4 production.

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“…Alves et al (2003) have promoted the in vitro production of bovine embryos because of the ease of incorporation into livestock production. However, a major hurdle in mass livestock production and a major cause of economic loss is the high embryonic and fetal mortality rate during the first trimester of gestation (Reichenbach et al 2003; prenatal mortality, a higher frequency of male fetuses, fetal oedema, abnormal organ growth (including the heart, brain, spinal cord and skeletal muscle), liver steatosis and abnormalities in biochemical parameters (Behboodi et al 1995;Young et al 1998;Hill et al 1999;Wells et al 1999;De Sousa et al 2001;Bertolini et al , 2004Matsuzaki and Shiga 2002;Ogura et al 2002;Chavatte-Palmer et al 2004, 2012Farin et al 2004Farin et al , 2006Sakai et al 2005;Drost 2007;Lonergan et al 2007;Miglino et al 2007;Campos et al 2010). In particular, it is generally accepted that morphological and physiological differences in embryos derived from oocytes fertilised in vitro may be caused by culture conditions (Stringfellow and Seidel 1998;Hansen et al 2010;Block et al 2011;Saadeldin et al 2011).…”

Section: Introductionmentioning

confidence: 99%

“…The exact reasons for this low efficiency are not known but have been investigated extensively in terms of genomic reprogramming. The results of these studies indicate complex changes in the patterns of epigenetic processes, especially in cloned embryos, including reduced expression of genes such as FGF4, NANOG, CASP genes and those responsible for trophoblast differentiation, and overexpression of IFNT and genes coding for angiogenetic factors, among others (Mundim et al 2009;Campos et al 2010;Rodríguez-Alvarez et al 2010;Leidenfrost et al 2011;Ripamonte et al 2011;Gong et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Development of bovine embryos derived from reproductive techniques

Alberto

Meirelles

Perecin

et al. 2013

Reprod. Fertil. Dev.

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Assisted reproduction techniques have improved agricultural breeding in the bovine. However, important development steps may differ from the situation in vivo and there is a high mortality rate during the first trimester of gestation. To better understand these events, we investigated the development of embryos and fetal membranes following fixed-time AI (FTAI), IVF and nuclear transfer (NT). The onset of yolk-sac development was not normal in cloned embryos. Later steps differed from conditions in vivo in all three groups; the yolk-sac was yellowish and juxtaposed with the amniotic membrane. Vascularisation of the chorioallantoic membrane was relatively late and low in NT gestations, but normal in the others. The overall development of the embryos was normal, as indicated by morphology and regression analysis of growth rate. However, NT conceptuses were significantly smaller, with the livers in some embryos occupying the abdominal cavity and others exhibiting heart abnormalities. In conclusion, the yolk-sac and the cardiovascular system seem to be vulnerable to morphogenetic alterations. Future studies will focus on gene expression and early vascularisation processes to investigate whether these changes may be responsible for the high incidence of intrauterine mortality, especially in clones.

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Somatic cell nuclear transfer is associated with altered expression of angiogenic factor systems in bovine placentomes at term

Cited by 20 publications

References 39 publications

Placental Evolution within the Supraordinal Clades of Eutheria with the Perspective of Alternative Animal Models for Human Placentation

Placental Evolution within the Supraordinal Clades of Eutheria with the Perspective of Alternative Animal Models for Human Placentation

Glucose transporter 1 expression accompanies hypoxia sensing in the cyclic canine corpus luteum

Development of bovine embryos derived from reproductive techniques

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