Somatic cell hybridization of human tumor samples

O’Donnell, Robert; Leary, James F.; Penney, David P.; Budd, Hollis S.; Marquis, Diana; Spennacchio, Janice; Henshaw, Edgar C.; McCune, Craig S.

doi:10.1007/bf01534908

Cited by 3 publications

(1 citation statement)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Other laboratories studying immortal-immortal human hybrids have also reported hybrids having limited division (18)(19)(20). However, the emphasis of the studies was on the tumorigenic behavior of the hybrids and minimum attention was paid to limited division hybrids.…”

Section: Discussionmentioning

confidence: 99%

Genetic analysis of indefinite division in human cells: identification of four complementation groups.

Pereira‐Smith

Smith

1988

Proc. Natl. Acad. Sci. U.S.A.

297

155

View full text Add to dashboard Cite

Hybrids obtained following fusion of normal human diploid fibroblasts with different immortal human cell lines exhibited limited division potential. This led to the conclusion that the phenotype of cellular senescence is dominant and that immortal cells arise as a result of recessive changes in the growth control mechanisms of the normal cell. We have exploited the fact that immortality is recessive and, by fusing immortal human cell lines with each other, assigned 21 cell lines to at least four complementation groups for indefinite division. A wide variety of cell lines was included in the study to determine what parameters, if any, would affect complementation group assignment. The results indicate that cell type, embryonal layer of origin, and type of tumor do not affect group assignment. There does not appear to be any correlation between expression of an activated oncogene and group assignment. However, all of the immortal simian virus 40-transformed cell lines studied (with the exception of one xerodermapigmentosum fibroblast-derived line) assign to the same group, indicating that this virus immortalizes various human cells by the same processes. The assignment of immortal human cells to distinct'groups provides the basis for a focused approach to determine the genes important in normal growth regnlation that have been modified in immortal cells.

show abstract

Section: Discussionmentioning

confidence: 99%

Genetic analysis of indefinite division in human cells: identification of four complementation groups.

Pereira‐Smith

Smith

1988

Proc. Natl. Acad. Sci. U.S.A.

297

155

View full text Add to dashboard Cite

show abstract

Polyethylene glycol significantly enhances the transfer of membrane immunoblotting

Zeng

Suzuki

Alpert

1990

Analytical Biochemistry

View full text Add to dashboard Cite

Could gastrointestinal tumor-initiating cells originate from cell-cell fusionin vivo?

Zhou¹,

Cheng²,

Feng³

et al. 2021

WJGO

View full text Add to dashboard Cite

Tumor-initiating cells (TICs) or cancer stem cells are believed to be responsible for gastrointestinal tumor initiation, progression, metastasis, and drug resistance. It is hypothesized that gastrointestinal TICs (giTICs) might originate from cell-cell fusion. Here, we systemically evaluate the evidence that supports or opposes the hypothesis of giTIC generation from cell-cell fusion both in vitro and in vivo . We review giTICs that are capable of initiating tumors in vivo with 5000 or fewer in vivo fused cells. Under this restriction, there is currently little evidence demonstrating that giTICs originate from cell-cell fusion in vivo . However, there are many reports showing that tumor generation in vitro occurs with more than 5000 fused cells. In addition, the mechanisms of giTIC generation via cell-cell fusion are poorly understood, and thus, we propose its potential mechanisms of action. We suggest that future research should focus on giTIC origination from cell-cell fusion in vivo, isolation or enrichment of giTICs that have tumor-initiating capabilities with 5000 or less in vivo fused cells, and further clarification of the underlying mechanisms. Our review of the current advances in our understanding of giTIC origination from cell-cell fusion may have significant implications for the understanding of carcinogenesis and future cancer therapeutic strategies targeting giTICs.

show abstract

Somatic cell hybridization of human tumor samples

Cited by 3 publications

References 15 publications

Genetic analysis of indefinite division in human cells: identification of four complementation groups.

Genetic analysis of indefinite division in human cells: identification of four complementation groups.

Polyethylene glycol significantly enhances the transfer of membrane immunoblotting

Could gastrointestinal tumor-initiating cells originate from cell-cell fusionin vivo?

Contact Info

Product

Resources

About