Somatic antigens of Pseudomonas aeruginosa. The structure of O-specific polysaccharide chains of the lipopolysaccharides from P. aeruginosa II (Sandvik) and V (IM-1, Verder-Evans)

Abstract

The lipopolysaccharides from two serologically related strains of Pseudomonas aeruginosa I1 (Sandvik classification) and V (IM-1, Verder-Evans classification) were isolated by the Westphal method and cleaved with 1 % acetic acid. The 0-specific polysaccharide from Sandvik I1 lipopolysaccharide involved L-rhamnose, N-acetyl-D-quinovosamine, and N-acetyl-D-galactosaminuronic acid in the ratio 2: 1 : 1, as well as 0-acetyl groups. The Verder-Evans V 0-specific polysaccharide was found to contain just the same sugars and, in addition, D-glucose, the monosaccharide ratio being 2: 1 : 1 : 1. On the basis of methylation analysis, Smith degradation, solvolysis with anhydrous hydrogen fluoride and 'H and I3C nuclear magnetic resonance data, it was concluded that the polysaccharides have identical main chains, and there were established the following structures of their repeating units:The data obtained allow us to substantiate the combining of the strains studied into one 0-serogroup as two individual 0-serotypes.An opportunistic pathogen Pseudomonas aeruginosa is known as a serologically heterogeneous species, and many serological schemes have been proposed for its classification (reviews [l, 21). On the basis of the classifications of Linyi [3] and Habs [4] the new most complete scheme has been compiled 111. In establishing the correlation between the strains from various classifications [I] it was found that the serogroup I1 of Sandvik's scheme [5] and the serogroup V (IM-1) of Verder-Evans' scheme [6], in contrast to the other groups of schemes, lack any counterparts in the classifications of Lanyi and Habs. Also, the serogroups SandvikII and Verder-Evans V turned out to be serologically interrelated, and were combined in the new compiled scheme into one serogroup 013 as serotypes 013a,13b and 013a,13c respectively.Immunospecificity of gram-negative bacteria is defined by the structure of polysaccharide chain of 0-antigens and, thus, chemical and immunochemical studies of P. aeruginosa lipopolysaccharides should provide a molecular basis for the classification of strains of this species. The present work Correspondence to Yu. A. Knirel, Institut Organicheskoj Khimii imeni N. D. Zelinskogo, Akademiya Nauk S.S.S.R., Leninskjj prospekt 47, Moskva, USSR-117913Abbreviations. QuiNAc, 2-acetamido-2,6-dideoxyglucose (Nacetylquinovosamine); GalNAcA, 2-acetamido-2-deoxygalacturonic acid (N-acetylgalactosaminuronic acid). deals with the establishing of the structures of 0-specific polysaccharides of P. aeruginosa I1 (Sandvik) and V (VerderEvans). MATERIALS AND METHODS Miscellaneous methods'H and I3C nuclear magnetic resonance (NMR) spectra were run on WM-250 and AM-300 (Bruker) instruments, respectively, with samples in D 2 0 at 60 "C (polysaccharides) and 30°C (oligosaccharides) and acetone (6, = 2.23 ppm) or methanol (6, = 50.15 ppm) as internal standard. Optical rotations were measured with a polarimeter EPO-1 (USSR) and solutions in water at 20°C. Solutions were freeze-dried or rotary evaporated in vacuo at 40°C. Methy...

K19 capsular polysaccharide of Acinetobacter baumannii is produced via a Wzy polymerase encoded in a small genomic island rather than the KL19 capsule gene cluster

“…The sites of glycosylation were confirmed by significant downfield displacements of the 13 C NMR signals for the linkage carbons, namely, C-3 of units A and C and C-4 of unit B, as compared with their positions in the non-substituted monosaccharides (Knirel et al, 1986;Lipkind et al, 1988). Analysis of the displacements of the signals for the linkage and neighbouring carbons using known regularities (Knirel et al, 1987;Shashkov et al, 1988) showed that all constituent monosaccharides have the same absolute configuration, namely the D configuration. Therefore, the K19 CPS of strain 28 has the structure shown in Fig.…”

WbpO, a UDP-N-acetyl-d-galactosamine Dehydrogenase from Pseudomonas aeruginosa Serotype O6

“…Structural analysis of polysaccharides with mass spectrometry, high performance liquid chromatography (10), and NMR (11)(12)(13)(14)(15)(16) has indicated that ␣-D-GalNAcA is one of the most common acidic sugars identified in the O-antigens or capsular polysaccharides of virulent Gram-negative bacteria. Reddy et al (11) suggested that bacterial capsules and LPS containing acidic sugar repeats play an important part in bacteria and host cells interactions.…”

Structure of the O-polysaccharide ofProteus penneri28 andProteus vulgarisO31 and classification ofP. penneri26 and 28 inProteusserogroup O31

“…the d ‐configuration of GlcNAc II (compare the corresponding glycosylation effects of −2.0 and +0.7 p.p.m. in α1→3‐linked disaccharides of 2‐acetamido‐2‐deoxy‐ l ‐ and d ‐galacturonic acid with d ‐QuiNAc, respectively [23]). This finding confirmed finally 2‐acetamido‐3‐ O ‐[( S )‐1‐carboxyethyl]‐2‐deoxy‐ d ‐glucose ( N ‐acetylisomuramic acid).…”