Somaclonal variation in olive ( Olea europaea L.) plants regenerated via somatic embryogenesis: Influence of genotype and culture age on phenotypic stability

Bradaï, Fatiha; Sánchez-Romero, Carolina; Martı́n, Carmen

doi:10.1016/j.scienta.2016.10.031

Cited by 36 publications

(30 citation statements)

References 24 publications

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“…Other studies have indicated that the genotype/species plays an important role in the frequency of genetic alterations during somatic embryogenesis (Burg et al 2007). The genotype, conditions and culture of the in vitro culture are considered to be parameters that influence genetic stability; however, some studies do not show a significant effect from these factors (Burg et al 2007, Carloni et al 2014, Mamedes-Rodrigues et al 2018. In the present study, although the explants showed different responses to the time of propagation, in general, we observed a reduction in genetic fidelity with increased cycles of induction at 2.26 μM 2,4-D (≈3 %).…”

Section: Resultscontrasting

confidence: 53%

“…8 and 9), genetic distances among explants varied from 0.02 to 0.18 with 2.26 μM 2,4-D while from 0.05 to 0.13 at 4.52 μM 2,4-D. The differential response of explants might be attributable to differences in sensitivity to the stresses caused by in vitro culture (Bradaï et al 2016). Other studies have indicated that the genotype/species plays an important role in the frequency of genetic alterations during somatic embryogenesis (Burg et al 2007).…”

Section: Resultsmentioning

confidence: 94%

“…(Fourré et al 1997, Lopes et al 2006, Valladares et al 2006, Triqui et al 2008, Yang et al 2008, Mehta et al 2011, Rai et al 2012, Bobadilla et al 2013, Li et al 2014, Carra et al 2016, Morais-Lino et al 2016, Naz et al 2016, Vinoth and Ravindhran 2016. It is possible that the explants selected may be less susceptible to genetic instability (DeVerno et al 1999) and, as a consequence, the results differ from those reported by others using the same morphogenetic route with intermediate levels of variation (17 -31 %) (Müller et al 1990, López et al 2004, Wilhelm et al 2005, Burg et al 2007, Prado et al 2010, Dey et al 2015.…”

Section: Resultsmentioning

confidence: 99%

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Induction of somatic embryogenesis and evaluation of genetic stability in regenerated plants of Magnolia dealbata

Chávez-Cortázar¹,

Mata-Rosas²,

Oyama³

et al. 2020

Biol. plant.

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The utility of plant tissue culture for the mass propagation of trees is well known, but continuous in vitro multiplication of plant material may increase the possibility of somaclonal variation; therefore, it is essential to evaluate the genetic integrity of regenerants from species-specific in vitro protocols prior to mass production and implementation. The objectives of this study were: 1) to determine the effect of 2,4-dichlorophenoxyacetic acid (2,4-D) concentration over two cycles of secondary somatic embryogenesis in Magnolia dealbata; and 2) to verify the genetic stability of the regenerants obtained. The embryogenic response was not significantly affected by the concentration of 2,4-D but did vary across cycles of induction. The addition of 4.52 μM 2,4-D induced the highest total number of embryos (100.5), the mean number of somatic embryos (25.1) and somatic embryos per explant (80.6). In both 2,4-D concentration (2.26 or 4.52 μM), genetic integrity between the donor and the propagated clones was 0.90, and the low genetic instability (≤ 0.10 in both PGR treatments) might be due to effect of cyclic somatic embryogenesis or the different response of the explants at stress in in vitro culture conditions. However, it is necessary to examine more cell lines and somatic embryogenesis cycles.

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Section: Resultscontrasting

confidence: 53%

Section: Resultsmentioning

confidence: 94%

Section: Resultsmentioning

confidence: 99%

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Induction of somatic embryogenesis and evaluation of genetic stability in regenerated plants of Magnolia dealbata

Chávez-Cortázar¹,

Mata-Rosas²,

Oyama³

et al. 2020

Biol. plant.

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“…During the past three decades, additional researchers have studied the induction, proliferation, maturation, and germination of SEs (Cornu and Jay-Allemand, 1989;Tulecke et al, 1988;Vahdati et al, 2006). Despite all the benefits that somatic embryos have for biotechnological advancements, plantlets regenerated from somatic embryos could exhibit somaclonal variation (Brada€ ı et al, 2016). Heritable adverse changes due to somaclonal variation can be a problematic in tree-breeding programs, which typically encounter long juvenile phases before phenotypic selection of mature elite genotypes, a delay period requiring substantial investment of time and money (Fourr e et al, 1997).…”

mentioning

confidence: 99%

“…Therefore, homogeneity and trueness-to-type of regenerated plantlets should be checked. Several methods are available for this analysis, including chromosome determination, flow cytometry, molecular markers, and biochemical and phenotypic analyses (Brada€ ı et al, 2016;Harding, 2004;Sadat-Hosseini et al, 2011). Molecular analyses and flow cytometry have been used in many species to evaluate the trueness-to-type of plantlets regenerated via somatic embryogenesis.…”

mentioning

confidence: 99%

Germination of Persian Walnut Somatic Embryos and Evaluation of their Genetic Stability by ISSR Fingerprinting and Flow Cytometry

Sadat-Hosseini¹,

Vahdati²,

Leslie³

2019

horts

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Somatic embryos (SEs) can play important roles in genetic manipulation and breeding. They can be used as targets for induced mutagenesis, as material for cryopreservation and germplasm conservation, and for transformation or gene editing in support of plant improvement and proof of gene function. However, germination rates of walnut (Juglans regia) SEs are low, and the genetic stability of plantlets regenerated from them has not been explored. Here, we studied first the effects of gibberellic acid (GA3) and low temperature storage (LTS) on germination of walnut somatic embryos. Second, we assessed the genetic fidelity of plantlets regenerated from these SEs by comparing them to each other and to their cultivar of origin. Results showed that GA3 and LTS increased the walnut SE germination rate. The best rate was observed when SEs were subjected to LTS for 60 d followed by culture on a medium with either 1 or 3 mg·L−1 GA3 (56.6% and 46.6% germination respectively). Genetic stability was evaluated, using flow cytometry and 15 sets of ISSR primers. Flow cytometry indicated that all samples (i.e., regenerated and parental counterpart) showed the same peak. Amplified fragments of inter simple sequence repeats (ISSR) primers ranged in size from ≈200 to 1800 bp. All ISSR profiles of regenerants were monomorphic. Results did not show any genetic differences among plantlets regenerated from SEs or from their parental counterpart. Due to this apparent genetic stability, walnut SEs can be useful for genetic transformation and germplasm conservation.

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