Somaclonal Variation in Improvement of Agricultural Crops: Recent Progress

Manoj, K.

doi:10.1007/978-981-16-2339-4_6

Cited by 15 publications

(6 citation statements)

References 74 publications

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“…With respect to the work analyzed, this was reflected in the lower genetic similarity obtained in the MS + KIN medium than the MS "0" in relation to DP plants. The reason for such a situation, as indicated by Gao et al [62], could be attributed to the influence of PGRs that increase the multiplication rate and induce adventitious shoots or existing genetic variation in cultured cells or tissues [31].…”

Section: Molecular Analysismentioning

confidence: 99%

“…Since their development, SCoT markers have been applied in population genetics and genetic diversity analysis in a wide range of plants, like cereals, legumes, fruit and vegetable crops, medicinal plants, forest trees, ornamental plants, and other economically important plants [26][27][28][29]. This type of marker has been used to analyze the genetic fidelity of tissue culture-raised plants, which is essential before its commercialization, particularly for elite and superior genotypes, where the regenerant is expected to be true to type [24,[30][31][32]. Publications strictly on Scutellaria baicalensis, both those relating to the micropropagation technique and the assessment of genetic diversity using genetic markers, are very rare.…”

Section: Introductionmentioning

confidence: 99%

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The Influence of Cytokinin on the Multiplication Efficiency and Genetic Stability of Scutellaria baicalensis Regenerants in In Vitro Culture Conditions

Dyduch-Siemińska,

Gawroński

2024

Applied Sciences

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The efficiency and method of regeneration in in vitro culture conditions depend primarily on the plant growth regulators (PGRs) used. Even growth regulators belonging to one group may have different effects, stimulating the process of direct or indirect organogenesis, thus possibly disturbing the genetic stability among regenerants. The main aim of this study was to identify the genetic stability of Scutellaria baicalensis regenerates obtained by in vitro culture method using start codon targeted (ScoT) markers. S.baicalensis nodal explants were regenerated on MS medium supplemented with kinetin (KIN) at concentrations of 0.25, 0.5, 1.0, and 2.0 mg × dm−3 or benzylaminopurine (BAP)—0.25, 0.5, 1.0, and 2.0 mg × dm−3. The effects of the number of propagated shoots, length, number of nodes, and fresh mass of regenerants were assessed. Moreover, the genetic stability of the regenerants was analyzed using start codon targeted (SCoT) markers. Direct shoot organogenesis was observed on an MS medium containing kinetin, while indirect shoot induction occurred on an MS medium supplemented with BAP. The highest average number of shoots (3.6) was achieved for the MS + KIN medium at a concentration of 0.25 and 5.8 for the MS + BAP 1.0 medium. The average length and average number of nodes were the highest on the MS + BAP 0.25 medium (50.0 and 6.0, respectively), while the lowest values of these features were observed on the MS + KIN 2.0 medium (40.3 and 4.9, respectively). A total of 111 amplified bands were exhibited by SCoT primers. Three of the analyzed primers revealed four unique genotype-specific markers. The average percentage of polymorphism obtained was 36.7%. The analysis of genetic similarity revealed a high level of genetic similarity between the donor plant and regenerants obtained on MS “0” (medium without the addition of phytohormones). A slightly lower value of genetic similarity was observed for regenerants obtained by direct organogenesis (MS + KIN medium at all concentrations). Indirect shoot organogenesis observed on the MS + BAP medium (all concentrations) resulted in the highest differentiation, both in relation to the donor plant and MS “0” regenerants. The results of our work indicate that, in the case of S. baicalensis, the maintenance of genetic stability depends primarily on the presence of the cytokinin type in the medium.

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Section: Molecular Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Influence of Cytokinin on the Multiplication Efficiency and Genetic Stability of Scutellaria baicalensis Regenerants in In Vitro Culture Conditions

Dyduch-Siemińska,

Gawroński

2024

Applied Sciences

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“…Increased rice production therefore contributes to world hunger eradication, poverty alleviation, food security and economic development. Many efforts have been made to develop high-yielding cultivars with better nutritional quality through traditional and mutation breeding, extensive crossing, and somatic clonal variation ( Rai 2022). Despite all these efforts, Pakistan's average rice yield per unit area is much lower and even lower than many neighboring countries (Li et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Synergistic Effect of Casein Hydrolysate and 2,4-D on in Vitro Callogenesis and Subsequent Regeneration in Rice (Oryza Sativa L.)

2024

Trends Biotechnol Plant Sci

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Even after three decades of invention, plastid transformation has been very difficult especially for monocot crops. Difficulty in attaining, efficient in vitro regeneration is one of the many factors affecting plastid transformation. Hence, present study was planned to investigate the impact of 2,4-D and casein hydolysate on in vitro callogenesis and subsequent regeneration of different Pakistani rice cultivars. For this purpose, mature seeds of six Pakistani genotypes IR-6, KSK-133, KSK-434, KS-282, PK-386 and Super Basmati were used. Scutellum from mature rice seeds were incubated on MS medium augmented with 2,4-D (1-5mg/L) to study callogenesis and subsequent regeneration response. Maximum callus formation was obtained in variety IR-6 (2mg/L 2,4-D) and callus size was found increasing with increasing 2,4-D concentration. Later, synergistic effect of casein hydrolysate and 2,4-D was investigated in two steps. Firstly, effect of 2,4-D in combination with 6mg/L casein hydrolyste was observed. Maximum callogenesis was found in IR-6 at 2mg/L 2,4-D and 6mg/L casein hydrolysate. Secondly, effect of casein hydrolysate (4-8mg/L) with 2,4-D 2mg/L was examined and maximum callus formation was found in variety IR-6 (casein hydrolysate 4mg/L + 2,4-D 2mg/L). Then, calli were shifted to different combinations of Plant Growth Regulators (PGRs) i.e. NAA + Kinetin and IBA + BAP to optimize regeneration. Differential response of varieties was examined. Maximum regeneration was observed in PK-386 (2.5 plantlets/callus).

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“…Nevertheless, Thailand’s policy on GM plants prohibits all open field trials of transgenic plants. Genetic variability created by tissue culturing provides a new source of variation (within a species) for use in crop development ( Rai, 2022 ). Tissue culture-mediated selection via the use of somatic embryos (SE) or embryogenic callus (EC) can be a powerful tool for the selection of excellent salt tolerance at the cellular level, because it allows the control of the homogeneity of the stress.…”

Section: Introductionmentioning

confidence: 99%

In vitro and in vivo screening for the identification of salt-tolerant sugarcane (Saccharum officinarum L.) clones: molecular, biochemical, and physiological responses to salt stress

Laksana

Sophiphun

Chanprame

2023

Saudi Journal of Biological Sciences

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Somaclonal Variation in Improvement of Agricultural Crops: Recent Progress

Cited by 15 publications

References 74 publications

The Influence of Cytokinin on the Multiplication Efficiency and Genetic Stability of Scutellaria baicalensis Regenerants in In Vitro Culture Conditions

The Influence of Cytokinin on the Multiplication Efficiency and Genetic Stability of Scutellaria baicalensis Regenerants in In Vitro Culture Conditions

Synergistic Effect of Casein Hydrolysate and 2,4-D on in Vitro Callogenesis and Subsequent Regeneration in Rice (Oryza Sativa L.)

In vitro and in vivo screening for the identification of salt-tolerant sugarcane (Saccharum officinarum L.) clones: molecular, biochemical, and physiological responses to salt stress

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