Somaclonal Genetics of Forest Trees

Ahuja, M. R.

doi:10.1007/978-94-015-9125-6_6

Cited by 17 publications

(3 citation statements)

References 86 publications

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“…The primary apprehension of micropropagation studies offers an integrated approach to develop consistent protocols that are easy, reproducible, and recommend a resourceful platform for genetic manipulation [34]. Axillary shoot bud multiplication is considered as a safest approach of micropropagation which suitably guarantees genetic stability of the regenerated plants.…”

Section: Discussionmentioning

confidence: 99%

Interactive Effects of Growth Regulators, Carbon Sources, pH on Plant Regeneration and Assessment of Genetic Fidelity Using Single Primer Amplification Reaction (SPARS) Techniques in Withania somnifera L.

Fatima

Ahmad

et al. 2015

Appl Biochem Biotechnol

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An improved and methodical in vitro shoot morphogenic approach through axillary bud multiplication was established in a drug yielding plant, Withania somnifera L. Effects of plant growth regulators [6-benzyladenine (BA), kinetin (Kin), 2-isopentenyladenine (2iP), and thidiazuron (TDZ)] either singly or in combination with α-napthalene acetic acid (NAA), indole-3-butyric acid (IBA), and indole-3-acetic acid (IAA) in Murashige and Skoog (MS) medium were tested. The highest regeneration frequency (90 %) with optimum number of shoots (32 ± 0.00)/explant were obtained on MS medium fortified with 2.5 μM 6-benzyladenine (BA) and 0.5 μM NAA and 30 g/l sucrose at pH 5.8. Among the tried TDZ concentrations, 0.5 μM resulted in maximum number of shoots (20.4 ± 0.40)/explant after 4 weeks of exposure. The proliferating shoot cultures established by repeated subculturing of the mother explants on the hormone-free medium produced the highest shoot number (29.4 ± 0.40) with shoot length (6.80 ± 0.12 cm)/explant at fourth subculture passage, which a decline in shoot proliferation was recorded. Different concentrations of NAA were tested for ex vitro rooting of microshoots. The maximum percentage of rooting 100 % with maximum roots (18.3 ± 0.1) was achieved in soilrite when basal portion of the microshoots were treated with 200 μM (NAA) for 15 min per shoot. The plantlets went through hardening phase in a growth chamber, prior to ex vitro transfer. The PCR-based single primer amplification reaction (SPAR) methods which include random amplified polymorphic DNA (RAPD) and direct amplification of minisatellite DNA (DAMD) markers has been used for assessment of genetic stability of micropropagated plantlets. No variation was observed in DNA fingerprinting patterns among the micropropagated and the donor plants illustrating their genetic uniformity.

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Section: Discussionmentioning

confidence: 99%

Interactive Effects of Growth Regulators, Carbon Sources, pH on Plant Regeneration and Assessment of Genetic Fidelity Using Single Primer Amplification Reaction (SPARS) Techniques in Withania somnifera L.

Fatima

Ahmad

et al. 2015

Appl Biochem Biotechnol

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“…It is generally assumed that axillary bud culture is the method offering least risk of genetic instability since meristems are more resistant to genetic changes than disorganized tissues [119,120]. Though it is sometimes species-specific International Journal of Biodiversity 3 [71,75], genetic fidelity or no significant genetic variation is indeed often observed in the regenerants from axillary bud culture systems according to known scientific literature.…”

Section: Axillary Bud Culture and Somaclonal Variationmentioning

confidence: 99%

Axillary Bud Proliferation Approach for Plant Biodiversity Conservation and Restoration

Ngezahayo

Liu

2014

International Journal of Biodiversity

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Due to mainly human population pressure and activities, global biodiversity is getting reduced and particularly plant biodiversity is becoming at high risk of extinction. Consequently, many efforts have been deployed to develop conservation methods. Because it does not involve cell dedifferentiation of differentiated cells but rather the development and growth of new shoots from preexisting meristems, the axillary bud proliferation approach is the method offering least risk of genetic instability. Indeed, meristems are more resistant to genetic changes than disorganized tissues. The present review explored through the scientific literature the axillary bud proliferation approach and the possible somaclonal variation that could arise from it. Almost genetic stability or low level of genetic variation is often reported. On the contrary, in a few cases studied to date, DNA methylation alterations often appeared in the progenies, showing epigenetic variations in the regenerated plants from axillary bud culture. Fortunately, epigenetic changes are often temporary and plants may revert to the normal phenotype. Thus, in the absence of genetic variations and the existence of reverting epigenetic changes over time, axillary bud culture can be adopted as an alternative nonconventional way of conserving and restoring of plant biodiversity.

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“…Improved in vitro shoot multiplication with rooting and reduced or total elimination of hyperhydricity have been reported in micropropagated plants using mT (Werbrouck et al 1996;Bairu et al 2007;Novikova et al 2020). Micropropagation from preformed structures such as shoot tips or axillary buds is generally assumed to be less prone to genetic instability, but somaclonal variation has been reported in tissue culture systems using this method (Ahuja 1998;Rani and Raina 2000;Bairu et al 2008). In vitro regenerated plants are not always free from genetic changes due to somaclonal variation (Pramanik and Datta 1986;Trolinder and Gooding 1987;Stelly et al 1988), so assessing genetic fidelity of regenerants is an important factor in the micropropagation of genetically uniform plants.…”

Section: Introductionmentioning

confidence: 99%

Micropropagation of Hibiscus moscheutos L. ‘Luna White’: effect of growth regulators and explants on nuclear DNA content and ploidy stability of regenerants

Sakhanokho

Islam‐Faridi

Babiker

et al. 2021

In Vitro Cell.Dev.Biol.-Plant

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Hibiscus moscheutos L., also known as hardy hibiscus, is valued for its medicinal and ornamental attributes. It is usually propagated via seeds or cuttings. The purpose of this investigation was to develop a dependable micropropagation for H. moscheutos ‘Luna White’. To that end, the effect of four explant types (leaf, root, node, shoot tip) and two growth regulators 6-benzylaminopurine (BA) and meta-Topolin (mT) (6-(3-hydroxybenzylamino) purine) on in vitro growth of H. moscheutos was investigated. Genetic stability of the in vitro grown plants was assessed using flow cytometry, and chromosome count was investigated. No shoots were obtained from leaf or root explants. An efficient protocol for micropropagation of H. moscheutos using two explant types, 2-node and shoot tip explants, and two cytokinins (BA and mT) capable of producing true-to-type regenerants was established. Both BA and mT can be used at 2 μM or 4 μM using either 2-node or shoot tip explants. No significant difference was found between the nuclear DNA contents of seed-derived and in vitro grown plants (P < 0.05). The mean 2C DNA and monoploid 1Cx-values of seed-derived plants were 3.25 ± 0.08 pg and 1.62 ± 0.04 pg, respectively, compared with 3.26 ± 0.06 pg and 1.63 ± 0.02 pg, respectively, for in vitro grown plants. The chromosome number of both seed-derived plants and regenerants was determined to be 2n = 2x = 38. The mature regenerants obtained were fertile and phenotypically similar to seed-derived plants.

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Somaclonal Genetics of Forest Trees

Cited by 17 publications

References 86 publications

Interactive Effects of Growth Regulators, Carbon Sources, pH on Plant Regeneration and Assessment of Genetic Fidelity Using Single Primer Amplification Reaction (SPARS) Techniques in Withania somnifera L.

Interactive Effects of Growth Regulators, Carbon Sources, pH on Plant Regeneration and Assessment of Genetic Fidelity Using Single Primer Amplification Reaction (SPARS) Techniques in Withania somnifera L.

Axillary Bud Proliferation Approach for Plant Biodiversity Conservation and Restoration

Micropropagation of Hibiscus moscheutos L. ‘Luna White’: effect of growth regulators and explants on nuclear DNA content and ploidy stability of regenerants

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