Solving the problem of shoot-tip necrosis in Harpagophytum procumbens by changing the cytokinin types, calcium and boron concentrations in the medium

Bairu, Michael W.; Jain, Neeru; Stirk, Wendy A.; Doležal, Karel; Staden, J. Van

doi:10.1016/j.sajb.2008.08.006

Cited by 73 publications

(54 citation statements)

References 30 publications

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“…Therefore, the plants need a period of acclimatization to correct the abnormalities. Our experiments showed that the adaptation of H. procumbens micropropagated plants to ex vitro conditions lasted several months and was longer than generally accepted (5-6 weeks) (Levieille and Wilson 2002;Bairu et al 2009). Pretreatment with TDZ has also effect on the quality of obtained regenerants.…”

Section: Acclimatization Of Plantlets Ex Vitromentioning

confidence: 57%

“…In vitro culture techniques can help overcome the problems. In literature there are some reports on the micropropagation of H. procumbens from nodal explants (Shushu 2001;Levieille and Wilson 2002;Kaliamoorthy et al 2008;Bairu et al 2009;Jain et al 2009) and shoot tips (Shushu 2001;Grąbkowska and Wysokińska 2009). The effects of different cytokinins (6-benzylaminopurine (BAP), thidiazuron (TDZ), zeatin, kinetin, meta-, ortho-, para-topolin and topolin riboside) on shoot multiplication in these studies have been also investigated.…”

Section: Introductionmentioning

confidence: 99%

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Influence of thidiazuron (TDZ) pretreatment of shoot tips on shoot multiplication and ex vitro acclimatization of Harpagophytum procumbens

2014

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The effects of thidiazuron (TDZ) pretreatment of shoot tips on Harpagophytum procumbens shoot proliferation and successive stages of micropropagation, i.e. rooting of regenerated shoots and acclimatization of plantlets to ex vitro conditions, were described in the present study. The best response in terms of shoot proliferation (about seven shoots/explant) and shoot length (3.2 ± 0.4 cm) was obtained when explants pretreated with 25 lmol L -1 TDZ for 6 h were cultured on Schenk and Hildebrandt medium containing indole-3-acetic acid (IAA) (0.57 lmol L -1 ) and 6-benzylaminopurine (BAP) (8 lmol L -1 ). Under these conditions, a 330 % increase in shoot multiplication over TDZ non-pretreatment culture was achieved and TDZ pretreatment shoots were longer compared to those in control culture (2.6 ± 0.3 cm). The TDZ pretreatment did not affect the percentage of rooted shoots, length of roots and number of roots formed per shoot. The rooted plantlets were transplanted from in vitro to pots with soil and grown during 1 year in the greenhouse. The hardening process was difficult and time-consuming. We found that the plants developed from the TDZ pretreated culture were superior to plants from non-pretreated culture in terms of survival rate and morphological features, such as shoot length, leaf size, flowering and earlier root tuberisation. Random amplified polymorphic DNA and inter-simple sequence repeat analyses of pretreatment with TDZ plants showed genetic similarity to non-pretreatment plants. We conclude that applying the strategy of initial explant pretreatment with TDZ may be valuable for the improvement in H. procumbens in vitro propagation.

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Section: Acclimatization Of Plantlets Ex Vitromentioning

confidence: 57%

Section: Introductionmentioning

confidence: 99%

Influence of thidiazuron (TDZ) pretreatment of shoot tips on shoot multiplication and ex vitro acclimatization of Harpagophytum procumbens

2014

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“…After 2 weeks of culturing, necrotic apices were observed in shoots growing in semisolid medium provided with either diffused or forced ventilation. This physiological disorder is commonly seen in in vitro cultures affecting a wide range of herbaceous and woody plants (Bairu et al, 2009a) and it is caused by a complex set of factors including plant growth regulators (Bairu et al, 2009b), calcium and boron deficiency (Hepler, 2005;Martin et al, 2007), medium type and salt strength (Jain et al, 2009;Takura and Kanwara, 2011), gelling agent (Singha et al, 1990), aeration (Bairu et al, 2009a), and pH (Pasqua et al, 2002). Our results indicated that the use of liquid medium through a TI system could overcome the shoot tip necrosis of H. heptaphyllus and promote growth and rooting of shoots.…”

Section: Resultsmentioning

confidence: 99%

Micropropagation of Handroanthus heptaphyllus (Vell.) Mattos from seedling explants

Duarte¹,

Sansberro²,

Luna³

2016

Afr. J. Biotechnol.

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Handroanthus heptaphyllus (Vell.) Mattos (Bignoniaceae), a tropical forest tree, is a source of wood suitable for the manufacture of fine furniture and chemical compounds with medicinal and insecticidal properties. Poor seed viability of this species limits the conventional propagation practice. In vitro germination of seeds was achieved in semi-solid Murashige and Skoog's (MS) medium. A disinfection protocol was optimized for germination and growth of seedlings in vitro. Proliferation of shoots from cotyledonary and stem nodal segments was achieved in semi-solid MS medium supplemented with 22.2 µM 6-benzyladenine. Temporary immersion culture promoted elongation and rooting of shoots and reduced the shoot tip necrosis compared with culture on semisolid medium (6.7±2.7 and 41.7±7.0%, respectively; P = 0.001). Plantlets derived from temporary immersion showed better performance during the acclimatization phase. This is the first report on micropropagation of H. heptaphyllus.

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“…Elongated shoots when subjected to rooting displayed shoot tip necrosis as observed during direct organogenesis (Chiruvella et al, 2013) suggesting the micropropagation of this plant is severely hampered by necrosis of shoots causing severe loss (Srivastava and Joshi, 2013;Bairu et al, 2009;Thakur et al, 2008;Martin et al, 2007;Kulkarni and Dsouza, 2000). We circumvented this problem by calcium supply to MS medium in the form of calcium nitrate (556 mg L -l ) and calcium pantothenate (1.0 mg L -l ) (Figure 4).…”

Section: In Vitro Rooting Of Shoots and Transfer Of Plantlets To Soilmentioning

confidence: 96%

In vitro callus induction and plantlet regeneration of Indian red wood, Soymida febrifuga A. Juss (Roxb.)

Kishore¹,

Mohammed²,

Rama³

2014

J. Med. Plants Res.

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Multiple shoots were regenerated from cotyledonary node, shoot tip explants derived callus by one stage process where caulogenesis occurred on the primary callus. However cotyledons, leaves and root segments gave rise to adventitious shoots from callus by two stage method of indirect organogenesis. High frequency of shoot differentiation was observed from cotyledon and leaf on MS medium fortified with BA (8.9 to 13.3 μM) in combination with NAA (0.54 μM) compared to other explants. Further, when elongated shoots were subjected to rooting, we observed shoot necrosis consistent to our previous findings. Similarly, we could overcome this problem by supplementing calcium to MS rooting medium. In vitro rooted plantlets were successfully transferred to soil, exhibiting a normal development. Altogether, our protocol provides a rapid technique for mass multiplication as well for ex situ conservation of this endemic medicinal plant.

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Solving the problem of shoot-tip necrosis in Harpagophytum procumbens by changing the cytokinin types, calcium and boron concentrations in the medium

Cited by 73 publications

References 30 publications

Influence of thidiazuron (TDZ) pretreatment of shoot tips on shoot multiplication and ex vitro acclimatization of Harpagophytum procumbens

Influence of thidiazuron (TDZ) pretreatment of shoot tips on shoot multiplication and ex vitro acclimatization of Harpagophytum procumbens

Micropropagation of Handroanthus heptaphyllus (Vell.) Mattos from seedling explants

In vitro callus induction and plantlet regeneration of Indian red wood, Soymida febrifuga A. Juss (Roxb.)

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