Solvents Production from a Mixture of Glucose and Xylose by Mixed Fermentation of Clostridium acetobutylicum and Saccharomyces cerevisiae

Qi, Gaoxiang; Huang, Chao; Chen, Xuefang; Lin, Xiaoqing; Chen, Xinde

doi:10.1007/s12010-015-1790-0

Cited by 21 publications

(2 citation statements)

References 14 publications

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“…acetobutylicum is a well-known species for conventional ABE (acetone-butanol-ethanol) fermentation and has been genetically adapted to produce more liquid biofuels and bulk chemicals in recent years. 21,22 As a model strain, C. acetobutylicum is well-studied and easy to genetically manipulate. Engineering C. acetobutylicum for extracellular production of saccharolytic enzymes is a possible solution to CBP.…”

Section: Introductionmentioning

confidence: 99%

Production of Butanol Directly from Hemicellulose through Secretory Expression of a Xylanase in Clostridium acetobutylicum

Wang

Cao

et al. 2020

Energy Fuels

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Butanol is an attractive second-generation biofuel as well as an important chemical. Production of butanol from lignocellulosic feedstock via consolidated bioprocessing has attracted increasing interest in recent years. However, this has been limited by the inefficient degradation of extracellular polysaccharides in butanol-producing strains. In this study, production of butanol directly from hemicellulose was achieved simply through overexpression of an indigenous xylanase in Clostridium acetobutylicum. The xylanase (XynB) encoded by CA_P0053 was highly soluble and fully secreted from C. acetobutylicum. The extracellular xylanase activity was increased 88-fold, and 4.03 g/L biobutanol was obtained from hemicellulose, which has been the highest yield yet reported from either hemicellulose or xylan. This study proved that the solventogenic C. acetobutylicum had great potential for consolidated production of biofuels. Understanding and applying its secretion mechanism will be of great significance in the future.

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Section: Introductionmentioning

confidence: 99%

Production of Butanol Directly from Hemicellulose through Secretory Expression of a Xylanase in Clostridium acetobutylicum

Wang

Cao

et al. 2020

Energy Fuels

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“…In ethanol production, lignocellulosic biomass which is the most abundant raw material has been widely used [1]. Due to its projected positive attributes in terms of economic, environmental, and social sustainability, cellulosic ethanol has been widely regarded as a promising alternative liquid fuel [2].…”

Section: Introductionmentioning

confidence: 99%

Co-generation of ethanol and l-lactic acid from corn stalk under a hybrid process

Wang

Liu

Cai

et al. 2018

Biotechnol Biofuels

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BackgroundCorn stover, as one important lignocellulosic material, has characteristics of low price, abundant output and easy availability. Using corn stover as carbon source in the fermentation of valuable organic chemicals contributes to reducing the negative environmental problems and the cost of production. In ethanol fermentation based on the hydrolysate of corn stover, the conversion rate of fermentable sugars is at a low level because the native S. cerevisiae does not utilize xylose. In order to increase the conversion rate of fermentable sugars deriving from corn stover, an effective and energy saving biochemical process was developed in this study and the residual xylose after ethanol fermentation was further converted to l-lactic acid.ResultsIn the hybrid process based on the hydrolysate of corn stover, the ethanol concentration and productivity reached 50.50 g L−1 and 1.84 g L−1 h−1, respectively, and the yield of ethanol was 0.46 g g−1. The following fermentation of l-lactic acid provided a product titer of 21.50 g L−1 with a productivity of 2.08 g L−1 h−1, and the yield of l-lactic acid was 0.76 g g−1. By adopting a blank aeration before the inoculation of B. coagulans LA1507 and reducing the final cell density, the l-lactic acid titer and yield reached 24.25 g L−1 and 0.86 g g−1, respectively, with a productivity of 1.96 g L−1 h−1.ConclusionsIn this work, the air pumped into the fermentor was used as both the carrier gas for single-pass gas stripping of ethanol and the oxygen provider for the aerobic growth of B. coagulans LA1507. Ethanol was effectively separated from the fermentation broth, while the residual medium containing xylose was reused for l-lactic acid production. As an energy-saving and environmental-friendly process, it introduced a potential way to produce bioproducts under the concept of biorefinery, while making full use of the hydrolysate of corn stover.

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Solvent production from xylose

Finneran

Popović

2018

Appl Microbiol Biotechnol

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Xylose is the second most abundant sugar derived from lignocellulose; it is considered less desirable than glucose for fermentation, and strategies that specifically increase xylose utilization in wild type or engineered cells are goals for biofuel production. Issues arise with xylose utilization because of carbohydrate catabolite repression, which is the preferential utilization of glucose relative to xylose in fermentations with both pure and mixed cultures. Taken together the low substrate utilization rates and solvent yields with xylose compared to glucose, many industrial fermentations ignore the xylolytic portion of the reaction in lieu of methods to maintain high glucose. This is shortsighted given the massive potential for xylose generation from a number of sustainable biomass feedstocks, based on utilization of the hemicellulose fraction(s) that enter pretreatment. A number of strategies have been developed in recent years to address xylose utilization and solvent production from xylose in systems with just xylose, or in systems with mixtures of glucose plus xylose, which are more typical of pretreated lignocellulose. The approaches vary in terms of complexity, stability, and ease of introduction to existing fermentation infrastructure (i.e., so-called drop-in fermentation strategies). Some approaches can be considered traditional engineering approaches (e.g., change the reaction conditions), while others are more subtle cellular approaches to eliminate the impacts of catabolite repression. Finally, genetic engineering has been used to increase xylose utilization, although this can be considered a relatively nascent approach compared to manipulations completed to date for glucose utilization.

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Solvents Production from a Mixture of Glucose and Xylose by Mixed Fermentation of Clostridium acetobutylicum and Saccharomyces cerevisiae

Cited by 21 publications

References 14 publications

Production of Butanol Directly from Hemicellulose through Secretory Expression of a Xylanase in Clostridium acetobutylicum

Production of Butanol Directly from Hemicellulose through Secretory Expression of a Xylanase in Clostridium acetobutylicum

Co-generation of ethanol and l-lactic acid from corn stalk under a hybrid process

Solvent production from xylose

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