Solventogenesis in Clostridium aceticum producing high concentrations of ethanol from syngas

Arslan, Kübra; Bayar, B.; Abubackar, Haris Nalakath; Veiga, María C.; Kennes, Christian

doi:10.1016/j.biortech.2019.121941

Cited by 45 publications

(38 citation statements)

References 35 publications

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“…Nevertheless, the biomass growth of C. aceticum gradually decreased when the pH reached acidic conditions. Additionally, they observed that C. aceticum converted ethanol to acetic acid in the solventogenic phase as a reverse reaction (Arslan et al, 2019). From the perspective of their results, the growth of C. aceticum in the mixed culture might be promoted by the alkali pH.…”

Section: Quantification Of Clostridium Aceticum During the Reactor Operationmentioning

confidence: 96%

“…Even though the main product of C. aceticum is acetic acid, there are several other products such as ethanol, butyric acid, and acetone etc., based on the metabolic pathway type (Jones et al, 2016). Arslan et al (2019) investigated acetic acid and ethanol production by C. aceticum under acidic and alkali pH conditions (Arslan et al, 2019). Their results showed that C. aceticum had a fast biomass growth under pH 8 with 1.8 g acetic acid production in the first 44 h of the fermentation.…”

Section: Quantification Of Clostridium Aceticum During the Reactor Operationmentioning

confidence: 99%

“…Interestingly, the results stated that the dominant acid type, which was propionic acid, did not change by bioaugmentation. Although C. aceticum is not responsible for propionic acid production according to its metabolic pathway (Alexander and Weuster-Botz, 2017;Arslan et al, 2019), propionic acid production was enhanced by bioaugmentation of C. aceticum. The average percentage propionic acid concentration in the VFA mixture was 34 ± 23% in Phase A, 55 ± 13% in Phase B, and 49 ± 9% in Phase C. The dominant acid type was propionic acid in the control reactor during operation (Figure 4B).…”

Section: Volatile Fatty Acid Composition Shift At the Bioaugmented Reactormentioning

confidence: 99%

“…C. aceticum is strictly anaerobic and can grow both autotrophically and heterotopically (Poehlein et al, 2015). Because of its versatile growth ability, C. aceticum has been widely used for acetic acid production (Sim et al, 2007;Arslan et al, 2019;Riegler et al, 2019). In our previous study, we used C. aceticum for acetic acid production from semi-synthetic milk processing wastewater fermentation under alkali pH (Atasoy et al, 2020a).…”

Section: Introductionmentioning

confidence: 99%

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Bioaugmented Mixed Culture by Clostridium aceticum to Manipulate Volatile Fatty Acids Composition From the Fermentation of Cheese Production Wastewater

Atasoy

Çetecioğlu

2021

Front. Microbiol.

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Production of targeted volatile fatty acid (VFA) composition by fermentation is a promising approach for upstream and post-stream VFA applications. In the current study, the bioaugmented mixed microbial culture by Clostridium aceticum was used to produce an acetic acid dominant VFA mixture. For this purpose, anaerobic sequencing batch reactors (bioaugmented and control) were operated under pH 10 and fed by cheese processing wastewater. The efficiency and stability of the bioaugmentation strategy were monitored using the production and composition of VFA, the quantity of C. aceticum (by qPCR), and bacterial community profile (16S rRNA Illumina Sequencing). The bioaugmented mixed culture significantly increased acetic acid concentration in the VFA mixture (from 1170 ± 18 to 122 ± 9 mgCOD/L) compared to the control reactor. Furthermore, the total VFA production (from 1254 ± 11 to 5493 ± 36 mgCOD/L) was also enhanced. Nevertheless, the bioaugmentation could not shift the propionic acid dominancy in the VFA mixture. The most significant effect of bioaugmentation on the bacterial community profile was seen in the relative abundance of the Thermoanaerobacterales Family III. Incertae sedis, its relative abundance increased simultaneously with the gene copy number of C. aceticum during bioaugmentation. These results suggest that there might be a syntropy between species of Thermoanaerobacterales Family III. Incertae sedis and C. aceticum. The cycle analysis showed that 6 h (instead of 24 h) was adequate retention time to achieve the same acetic acid and total VFA production efficiency. Biobased acetic acid production is widely applicable and economically competitive with petroleum-based production, and this study has the potential to enable a new approach as produced acetic acid dominant VFA can replace external carbon sources for different processes (such as denitrification) in WWTPs. In this way, the higher treatment efficiency for WWTPs can be obtained by recovered substrate from the waste streams that promote a circular economy approach.

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Section: Quantification Of Clostridium Aceticum During the Reactor Operationmentioning

confidence: 96%

Section: Quantification Of Clostridium Aceticum During the Reactor Operationmentioning

confidence: 99%

Section: Volatile Fatty Acid Composition Shift At the Bioaugmented Reactormentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Bioaugmented Mixed Culture by Clostridium aceticum to Manipulate Volatile Fatty Acids Composition From the Fermentation of Cheese Production Wastewater

Atasoy

Çetecioğlu

2021

Front. Microbiol.

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“…A two-stage continuous cultivation of C. ljungdahlii also allowed to control acidogenesis and solventogenesis using the pH setpoint [ 247 ]. Similarly, solventogenesis was induced during batch cultivation of the acetogen C. aceticum by shifting the pH-value from 8.0 to 6.9 [ 10 ]. Interestingly, the pH value was also suggested to favor alcohol formation reactions and to hamper the formation of longer fatty acids like hexanoic acid during the cultivation of acetogenic clostridia [ 41 ].…”

Section: Solventogenic and Acetogenic Clostridiamentioning

confidence: 99%

Towards continuous industrial bioprocessing with solventogenic and acetogenic clostridia: challenges, progress and perspectives

Vees

Neuendorf

Pflügl

2020

Journal of Industrial Microbiology and Biotechnology

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The sustainable production of solvents from above ground carbon is highly desired. Several clostridia naturally produce solvents and use a variety of renewable and waste-derived substrates such as lignocellulosic biomass and gas mixtures containing H2/CO2 or CO. To enable economically viable production of solvents and biofuels such as ethanol and butanol, the high productivity of continuous bioprocesses is needed. While the first industrial-scale gas fermentation facility operates continuously, the acetone–butanol–ethanol (ABE) fermentation is traditionally operated in batch mode. This review highlights the benefits of continuous bioprocessing for solvent production and underlines the progress made towards its establishment. Based on metabolic capabilities of solvent producing clostridia, we discuss recent advances in systems-level understanding and genome engineering. On the process side, we focus on innovative fermentation methods and integrated product recovery to overcome the limitations of the classical one-stage chemostat and give an overview of the current industrial bioproduction of solvents.

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Enzymatic properties of ornithine decarboxylase from Clostridium aceticum DSM1496

Tan,

Gou,

Fan

et al. 2024

Biotech and App Biochem

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Clostridium aceticum DSM1496 is an acid‐resistant strain in which ornithine decarboxylase (ODC) plays a crucial role in acid resistance. In this study, we expressed ODC derived from C. aceticum DSM1496 in Escherichia coli BL21 (DE3) and thoroughly examined its enzymatic properties. The enzyme has a molecular weight of 55.27 kDa and uses pyridoxal‐5′‐phosphate (PLP) as a coenzyme with a Km = 0.31 mM. ODC exhibits optimal activity at pH 7.5, and it maintains high stability even at pH 4.5. The peak reaction temperature for ODC is 30°C. Besides, it can be influenced by certain metal ions such as Mn2+. Although l‐ornithine serves as the preferred substrate for ODC, the enzyme also decarboxylates l‐arginine and l‐lysine simultaneously. The results indicate that ODC derived from C. aceticum DSM1496 exhibits the ability to produce putrescine, cadaverine, and agmatine through decarboxylation. These polyamines have the potential to neutralize acid in an acidic environment, facilitating the growth of microorganisms. These significant findings provide a strong basis for further investigation into the acid‐resistant mechanisms contributed by ODC.

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Solventogenesis in Clostridium aceticum producing high concentrations of ethanol from syngas

Cited by 45 publications

References 35 publications

Bioaugmented Mixed Culture by Clostridium aceticum to Manipulate Volatile Fatty Acids Composition From the Fermentation of Cheese Production Wastewater

Bioaugmented Mixed Culture by Clostridium aceticum to Manipulate Volatile Fatty Acids Composition From the Fermentation of Cheese Production Wastewater

Towards continuous industrial bioprocessing with solventogenic and acetogenic clostridia: challenges, progress and perspectives

Enzymatic properties of ornithine decarboxylase from Clostridium aceticum DSM1496

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