Solventless injecction for packed column supercritical fluid chromatography

Dean, Thomas A.; POOLE, C

doi:10.1002/jhrc.1240121202

Cited by 22 publications

(4 citation statements)

References 9 publications

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“…O r SFC [7][8][9][10][11]. Among the papers describing the solventless injection technique published to date, various sets of conditions regarding sample precolumn temperature, purging gas flow rate, injection solvent composition, and stationary phase packing for the sample precolumn have been proposed.…”

Section: Liquid Sample Loadingmentioning

confidence: 99%

Characterization of a solventless injection interface for supercritical fluid chromatography

Gao¹,

Kibbey²

2000

Chromatographia

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KeyWordsSupercritical fluid chromatography Solventless injection Solvent-induced band broadening SummaryPacked column supercritical fluid chromatography (SFC) is capable of highly efficient separations of druglike molecules with less solvent consumption than HPLC. However, solute peaks in SFC are susceptible to broadening and distortion due to mismatch between the elution strength and viscosity of the injection solvent and the SFC mobile phase. The magnitude of solventinduced band broadening increases as the elution strength and viscosity of the sample solvent and mobile phase become more dissimilar and varies with the type of stationary phase used and the nature of the solute injected. A number of researchers have coupled supercritical fluid extraction with supercritical fluid chromatography in an attempt to remove solvent from injected samples, and thereby reduce peak broadening and distortion during SFC chromatography. While solventless injection is effective in overcoming solvent-induced band broadening in SFC, there have been few reports dealing with the optimization of the solvent removal process of this technique. In this work, we describe the influence of temperature, purge time, purging gas flow rate, stationary phase used in the sample precolumn, and injection solvent composition on solvent elimination and solute recovery.

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Section: Liquid Sample Loadingmentioning

confidence: 99%

Characterization of a solventless injection interface for supercritical fluid chromatography

Gao¹,

Kibbey²

2000

Chromatographia

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“…The major efforts towards solvent elimination have been exhibited in connection with the use of open tubular capillary columns, but Dean and Poole (1989) also made a study of similar techniques for packed microbore (1 mm i.d.) columns.…”

Section: Solvent Venting Under Reduced Pressurementioning

confidence: 99%

Applications of Supercritical Fluids in Industrial Analysis

Dean¹

1993

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“…Supercritical fluid extraction can be coupled to supercritical fluid chromatography using a series of switching valves and either a loop interface or an accumulator trap [257,260,[268][269][270][271][272][273]. In the loop interface the fluid from the extraction cell passes continuously through an injection loop and into a collection vessel.…”

Section: Supercritical Fluid Extractionmentioning

confidence: 99%

Sample Preparation for Chromatographic Analysis

Poole

1991

Chromatography Today

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Solventless injecction for packed column supercritical fluid chromatography

Cited by 22 publications

References 9 publications

Characterization of a solventless injection interface for supercritical fluid chromatography

Characterization of a solventless injection interface for supercritical fluid chromatography

Applications of Supercritical Fluids in Industrial Analysis

Sample Preparation for Chromatographic Analysis

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