Solvent properties governing protein partitioning in polymer/polymer aqueous two-phase systems

Cooperativity between various types of polar solute–solvent interactions in aqueous media

“…The ATPSs of the compositions shown below (see in Table 1) were prepared as previously described [4][5][6]. The phases were separated and used for solvatochromic analysis.…”

“…It has been shown recently that the solute specific coefficients may be determined for a given compound (including proteins) by analysis of partition coefficients of the compound in multiple ATPS with different polymers but the same ionic composition [4][5][6][15][16][17]. Once , ˛, ˇ, and c parameters in multiple ATPS are determined, the solute specific coefficients can be calculated by multiple linear regression analysis.…”

“…Our current understanding of aqueous solvent-solute interactions is very limited, however. Recognizing the difficulties in describing water and its interactions with solutes on the molecular level led us to propose a new approach to quantify and understand these interactions based on solute partitioning in aqueous two-phase systems (ATPS) [4][5][6].…”

“…The solute preferential distribution between the aqueous phases is mainly driven by different solute-solvent interactions [13][14][15][16][17] and it can be described as [4][5][6][15][16][17]:…”

Self-buffering and biocompatible ionic liquid based biological media for enzymatic research

“…The completion of reaction mixture was ensured by checking the pH of the reaction mixture. Aer the completion of reaction, the mixture was then evaporated at about [50][51][52][53][54][55][56][57][58][59][60] C by using a rotary evaporator (Panchum Evap., Chering Huei Co., Ltd, Taiwan) connected with a high capacity vacuum pump. To remove the excess buffer or any other impurities from the obtained product, one to one ratio mixture of ethanol and acetonitrile was added to the product and was vigorously stirred for about 1 hour at room temperature.…”