Solvent production byClostridium pasteurianum in media of high sugar content

Harris, Janet; Mulder, R.; Kell, Douglas B.; Walter, Robert P.; Morris, Jonathan

doi:10.1007/bf01078654

Cited by 21 publications

(10 citation statements)

References 9 publications

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“…4a and c is that most butanol was produced when cells were in the exponential phase, as also showed by Biebl [5]. This behaviour completely differs from the traditional ABE fermentation (C. acetobutylicum) [49] and also from the glucose fermentation by C. pasteurianum [50] in which, even though solvent production can be observed in the late exponential growth phase, most solvent accumulation occurs beyond it. A possible explanation is that the high degree of reduction of glycerol stimulates an early initiation of solvent production.…”

Section: Discussionmentioning

confidence: 81%

Influence of nutritional and operational parameters on the production of butanol or 1,3-propanediol from glycerol by a mutant Clostridium pasteurianum

2017

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Butanol and 1,3-propanediol (1,3-PDO) are simultaneously produced by Clostridium pasteurianum from glycerol. In this study, random chemical mutagenesis of C. pasteurianum DSM 525 was conducted to improve its tolerance to butanol. Selected nutritional and operational parameters were evaluated to identify strategies that favour the production of each metabolite. From those experiments, it was possible to isolate cells able to produce 22% more butanol than the parent strain in serum bottles. The supplementation of the culture medium with 2mgl of iron increased the production of butanol by 163%, and the optimum inoculum age was found to be 12hours. Overall, the experiments conducted in bioreactor led to lower butanol titers than in serum bottles, which is attributed to the higher pressure present in the bottles. At pH 6.0, N sparging notoriously favoured the production of biomass and 1,3-PDO, while a lower pH (5.0) led to a higher butanol yield, although growth was negatively affected. The results herein gathered allowed the identification of specific conditions that favour the production of either butanol or 1,3-PDO. Furthermore, it was found that N sparging is a suitable strategy to maximize the titer, yield and productivity of 1,3-PDO using C. pasteurianum.

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Section: Discussionmentioning

confidence: 81%

Influence of nutritional and operational parameters on the production of butanol or 1,3-propanediol from glycerol by a mutant Clostridium pasteurianum

2017

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“…However, Harris et al . reported that the species also could produce significant quantities of acetone, butanol, and ethanol, when grown in media of high glucose content. Nakas et al .…”

Section: Microbial Cultures For Acetone–butanol–ethanol Fermentationmentioning

confidence: 99%

“…This species was initially known as an acid producer that fermented carbohydrates to butyrate, acetate, CO 2 , and H 2 [83]. However, Harris et al [84] reported that the species also could produce significant quantities of acetone, butanol, and ethanol, when grown in media of high glucose content. Nakas et al [85] have reported conversion of algal biomass supported with 4%w/v glycerol to butanol, 1,3-propanediol and ethanol.…”

Section: Microbial Cultures For Acetone-butanol-ethanol Fermentationmentioning

confidence: 99%

Biobutanol: science, engineering, and economics

Ranjan

Moholkar

2011

Int. J. Energy Res.

151

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SUMMARYAmong several liquid alternative fuels, biobutanol has shown great promise because of its very similar properties to gasoline. This review provides an overview of research activities in acetone-butanol-ethanol (ABE) fermentation over the past two and a half decades. We have addressed seven important facets of ABE fermentation, viz. biochemistry, microbial cultures, alternative substrates, solvent recovery, fermentation mode and reactor designs, mathematical modeling, and economics. Development of mutant strains having higher yield, selectivity and tolerance to inhibition, and search for cheap alternative substrates for fermentation are most important thrust areas in biobutanol production. New and efficient processes have been developed for in situ removal and recovery of the ABE solvents. Several rigorous kinetic and physiological models for fermentation have been formulated, which form useful tool for optimization of the process. These research activities have been reviewed in this paper. Finally, we have summarized studies on the economic viability of large-scale ABE fermentation processes employing various process designs, substrates, and microbial cultures. With the use of new strains, inexpensive substrates, and superior reactor designs, economic potential of ABE fermentation has been found to be highly attractive. Research efforts in science, engineering, and economics of ABE fermentation have brought biobutanol close to commercialization as liquid alternate fuel.

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“…cultures. For example, when Clostridium pasteurianum are grown in high glucose concentrations significant quantities of alcohols are produced (Harris et al, 1986). Brosseau et al (1986) also reported that the hydrogen was produced in the cell growth phase, whereas the cells produced butanol at equilibrium.…”

Section: Introductionmentioning

confidence: 97%

Biohydrogen generation by mesophilic anaerobic fermentation of microcrystalline cellulose

Lay

2001

Biotech & Bioengineering

259

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Sixteen batch experiments were performed to evaluate the stability, kinetics, and metabolic paths of heat-shocked digester (HSD) sludge that transforms microcrystalline cellulose into hydrogen. Highly reproducible kinetic and metabolic data confirmed that HSD sludge could stably convert microcrystalline cellulose to hydrogen and volatile fatty acids (VFA) and induce metabolic shift to produce alcohols. We concluded that clostridia predominated the hydrogen-producing bacteria in the HSD sludge. Throughout this study the hydrogen percentage in the headspace of the digesters was greater than 50% and no methanogenesis was observed. The results emphasize that hydrogen significantly inhibited the hydrogen-producing activity of sludge when initial microcrystalline cellulose concentrations exceeded 25.0 g/L. A further 25 batch experiments performed with full factorial design incorporating multivariate analysis suggested that the ability of the sludge to convert cellulose into hydrogen was influenced mainly by the ratio of initial cellulose concentration (So) to initial sludge density (Xo), but not by interaction between the variables. The hydrogen-producing activity depended highly on interaction of So x (So/Xo). Through response surface analysis it was found that a maximum hydrogen yield of 3.2 mmol/g cellulose occurred at So = 40 g/L and So/Xo = 8 g cellulose/g VSS. A high specific rate of 18 mmol/(g VSS-d) occurred at So = 28 g/L and So/Xo = 9 g cellulose/g VSS. These experimental results suggest that high hydrogen generation from cellulose was accompanied by low So/Xo.

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Solvent production byClostridium pasteurianum in media of high sugar content

Cited by 21 publications

References 9 publications

Influence of nutritional and operational parameters on the production of butanol or 1,3-propanediol from glycerol by a mutant Clostridium pasteurianum

Influence of nutritional and operational parameters on the production of butanol or 1,3-propanediol from glycerol by a mutant Clostridium pasteurianum

Biobutanol: science, engineering, and economics

Biohydrogen generation by mesophilic anaerobic fermentation of microcrystalline cellulose

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