Solvent extraction of bacteriocins from liquid cultures

Abstract

. 2000. A solvent extraction method was developed to concentrate lacidin from the culture of Lactobacillus acidophilus OSU133. The new method concentrates the bacteriocin at the interface between chloroform and the aqueous culture of the producing bacterium. Compared with other extraction procedures, the new method effectively recovers higher bacteriocin yield and results in relatively clean preparations. Recovery of lacidin by the chloroform extraction procedure, compared with ammonium sulphate precipitation and cell acidi®cation methods, was >10-fold and about 100-fold greater, respectively. The new extraction procedure saves time and is easy to perform. This method is also effective in recovering subtilin, bacillicin, pediocin and nisin from cultures of Bacillus subtilis ATCC 6633, B. subtilis OSY1115/C, Pediococcus acidilactici PO2 and Lactococcus lactis ATCC 11454, respectively.

Insights into In Vivo Activities of Lantibiotics from Gallidermin and Epidermin Mode-of-Action Studies

“…Interestingly, L. lactis membranes have been reported to contain considerable proportions of phospholipids with 16, 18, and 19 C atoms; the average acyl chain length had been calculated to be 17.1 (25). In contrast, for several staphylococcal species (32), for S. aureus (48,50), and for Micrococcus luteus (51), a high proportion of C 15 and very little C 17 or longer acyl chains have been reported. Whether these figures are sufficient to explain the observed differences in pore formation capacity of gallidermin remains to be studied.…”

“…Producer strains were grown for 24 h. Cells were harvested by centrifugation (10,000 ϫ g, 10 min) and the peptides were extracted from the culture supernatant as described previously (15). Chloroform was added to the supernatant fluid (0.1:1, vol/vol), stirred vigorously for 1 h at 4°C, and centrifuged (10,000 ϫ g, 10 min) for phase separation.…”

Bacteriocins from lactic acid bacteria: purification, properties and use as biopreservatives

“…Usual methods for bacteriocins extraction are based on their affinity to organic solvents, their variation in solubility in concentrated salt solutions and at a given pH value. The presence of hydrophobic regions in bacteriocin molecules is essential for their activity against sensitive bacteria, since inactivation of microorganisms by bacteriocins depends on the hydrophobic interaction between the bacterial cells and bacteriocin molecules (Burlanek and Yousef, 2000). Some bacteriocins appear in their native state as aggregates with high molecular mass (ca.…”

In situ production of pediocin PA-1 like bacteriocin by different genera of lactic acid bacteria in soymilk fermentation and evaluation of sensory properties of the fermented soy curd

“…Simultaneously, the bacteriocin was extracted using chloroform (SRL, Mumbai, India) as described by Burianek and Yousef (2000). The crude bacteriocin (2 μl, 200 AU/mL) was added to freshly grown culture of L. monocytogenes ScottA (10 6 cfu/mL) and observed for its morphological changes by scanning electron microscope.…”