Solvent effects on emission yield and lifetime for coumarin laser dyes. Requirements for a rotatory decay mechanism

Jones, Guilford; Jackson, W. R.; Choi, Chol Yoo; Bergmark, William R.

doi:10.1021/j100248a024

Cited by 1,136 publications

(919 citation statements)

References 3 publications

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“…Fluorescence lifetimes for the three standards were also verified by TCSPC measurements using a Fluotime 200 lifetime spectrometer (PicoQuant, Germany) and compared favourably to the literature values [22][23][24], and with previous studies in this laboratory [25]. All measurements were made at room temperature and the standard solutions were not degassed or deoxygenated.…”

Section: Methodsmentioning

confidence: 65%

Frequency Domain Fluorescence Lifetime Study of Crude Petroleum Oils

2008

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Abstract:Frequency domain (FD) fluorescence lifetime data was collected for a series of 20 crude petroleum oils using a 405 nm excitation source and over a spectral range of ~426 to ~650 nm. Average fluorescence lifetimes were calculated using three different models: discrete multi-exponential, Gaussian distribution, and Lorentzian distribution. Fitting the data to extract accurate average lifetimes using the various models proved easier and less time consuming for the FD data than with Time Correlated Single Photon Counting (TCSPC) methods however the analysis of confidence intervals to the computed average lifetimes proved cumbersome for both methods. The uncertainty in the average lifetime was generally larger for the discrete lifetime multi-exponential model when compared to the distributionbased models. For the lifetime distributions, the data from the light crude oils with long lifetimes generally fit to a single decay term. Heavier oils with shorter lifetimes required This is the author version of the paper: Frequency Domain Fluorescence Lifetime Study of Crude Petroleum Oils. P. Owens, A.G. Ryder, and N.J.F. Blamey. Journal of Fluorescence, 18(5), 997-1006Fluorescence, 18(5), 997- , (2008. DOI: 10.1007/s10895-008-0330-5Page 2 of 21 multiple decay terms. The actual value for the average lifetime is more dependant on the exact fitting model employed than the data acquisition method used. Correlations between average fluorescence lifetimes and physical and chemical parameters of the crude oils were made with a view to developing a quantitative model for predicting the gross chemical composition of crude oils. It was found that there was no significant benefit gained by using FD over TCSPC other than more rapid data analysis in the FD case. For the FD data the Gaussian distribution model for fluorescence lifetime gave the best correlations with chemical composition allowing a qualitative correlation to some bulk oil parameters.

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Section: Methodsmentioning

confidence: 65%

Frequency Domain Fluorescence Lifetime Study of Crude Petroleum Oils

2008

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“…One rationale for this high fluorescence yield may be attribute to the rigidity of PMMA or high viscosity of PMMA. Because of the rigidity of PMMA or high viscosity of PMMA the rotational and vibrational relaxation of laser dye molecules is reduced and/or prevented, wherein the vibrational and rotational relaxation processes are the main reason for the radiative energy losses [6,7]. The non-radiative decay of the lowest excited singlet state S 1 directly to the ground state S 0 is generally responsible for the loss of fluorescence efficiency in organic dyes.…”

Section: Resultsmentioning

confidence: 99%

“…In solutions, the solvent environment determines important changes in the electro-optical properties of the spectrally active molecule compared to those in its gaseous phase. Several reports are now available on the correlation of UV absorption frequencies with the solvent parameters [7,8]. In the present paper a technique that could be used for sensing organic compounds is presented.…”

Section: Introductionmentioning

confidence: 99%

A Technique for Sensing Organic Compounds Using Fluorescence Maximum Shift In Laser Dyes

Dadge¹,

Thakur²

2017

J Laser Opt Photonics

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A technique that could be used for sensing organic compounds is presented. In ethanol, Rhodamine-6G (a laser dye) was dissolved to obtain a Rhodamine-ethanol (Rh-Eth) solution with the concentration of Rhodamine-6G being 0.005 M. Methanol (0.25 cm 3 ) was added to the Rh-Eth solution and the Rh-Eth-Me solution so obtained was pumped with a nitrogen laser. A blue shift in the fluorescence spectrum of the Rh-Eth-Me was observed as compared to the Rh-Eth solution without methanol. A similar blue shift was observed in the Rh-Eth solution when acetone and acetonitrile were separately added. When Rhodamine-6G and Coumarin460 were added with Polymethyl methacrylate (PMMA) in ethanol, a blue shift was observed in the fluorescence spectrum as compared to the Rh-6G and Cou460 in ethanol without PMMA. The phenomena of spectral shifts in the fluorescence spectrum can be used to sense organic compounds and a mechanism is discussed.

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“…Reference compounds used for calculation included naphthalene (Φ R = 0.23 for EtOH), 56 anthracene (Φ R = 0.27 for EtOH), 57 and coumarin 1 (Φ R = 0.73 for EtOH). 58 Standard molecule quantum yield measurements were performed in acetonitrile.…”

Section: ■ Discussionmentioning

confidence: 99%

Discovery of Small Molecules for Fluorescent Detection of Complement Activation Product C3d

et al. 2015

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Complement activation plays a major role in many acute and chronic inflammatory conditions. C3d, a terminal product of complement activation, remains covalently attached to cells and is an excellent biomarker of complementmediated inflammation. We employed a virtual highthroughput screening protocol to identify molecules with predicted binding to complement C3d and with intrinsic fluorescence properties to enable detection. Pharmacophore models were developed based on known C3d−ligand interactions and information from computational analysis of structural and molecular dynamics data. Iterative pharmacophore-based virtual screening was performed to identify druglike molecules with physicochemical similarity to the natural C3d ligand CR2. Hits from the pharmacophore screens were docked to C3d and ranked based on predicted binding free energies. Top-ranked molecules were selected for experimental validation of binding affinity to C3d, using microscale thermophoresis, and for their suitability to become molecular imaging agents, using fluorescence spectroscopy. This work serves as a foundation for identifying additional fluorescent molecules with high-affinity for C3d that will subsequently be explored as noninvasive in vivo diagnostics of complementmediated inflammation, for spatiotemporal monitoring of disease progression, and for targeting therapeutics to sites of inflammation.

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Solvent effects on emission yield and lifetime for coumarin laser dyes. Requirements for a rotatory decay mechanism

Cited by 1,136 publications

References 3 publications

Frequency Domain Fluorescence Lifetime Study of Crude Petroleum Oils

Frequency Domain Fluorescence Lifetime Study of Crude Petroleum Oils

A Technique for Sensing Organic Compounds Using Fluorescence Maximum Shift In Laser Dyes

Discovery of Small Molecules for Fluorescent Detection of Complement Activation Product C3d

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