Solution studies of the nucleic acid bases and related model compounds. Solubility in aqueous alcohol and glycol solutions

Thermochemistry of adenosine

“…Four earlier studies (18)(19)(20)(21) report results that lead to the saturation molality of adenosine; these are now summarized. Herskovits and Harrington (19) and Herskovits and Bowen (20) reported the same value for the solubility s of adenosine at T = 298.15 K. Their value s = 1.92 · 10 −2 mol · dm −3 was converted to the value m(sat) = 1.93 · 10 −2 mol · kg −1 by using the density ρ = 0.9989 kg · dm −3 calculated from the standard apparent molar volume of adenosine(aq) reported by Patel and Kishore.…”

“…Four earlier studies (18)(19)(20)(21) report results that lead to the saturation molality of adenosine; these are now summarized. Herskovits and Harrington (19) and Herskovits and Bowen (20) reported the same value for the solubility s of adenosine at T = 298.15 K. Their value s = 1.92 · 10 −2 mol · dm −3 was converted to the value m(sat) = 1.93 · 10 −2 mol · kg −1 by using the density ρ = 0.9989 kg · dm −3 calculated from the standard apparent molar volume of adenosine(aq) reported by Patel and Kishore. (22) Stockx et al (18) reported s = 1.69 · 10 −2 mol · dm −3 at T = 294.15 K. The density at this temperature was calculated (23) from the results of Patel and Kishore (22) and then used to calculate m(sat) = 1.70 · 10 −2 mol · kg −1 at this temperature.…”

“…We note that all of the previous results relied upon a spectrophotometric analysis for the determination of the concentration of adenosine in water. The studies of Stockx et al, (18) Herskovits and Harrington, (19) and of Herskovits and Bowen, (20) used the molar absorption coefficient reported by Beaven et al (25) Laurs et al (21) used a molar absorption coefficient taken from a biochemical handbook (26) which is not easily accessible. Thus, we do not know either the value or the source of the molar absorption coefficient cited in this handbook and used by Lauers et al (21) Nevertheless, the use of the same value for the molar absorption coefficient could, in part, account for the agreement in the values of m(sat) obtained from the earlier studies.…”

An association between chromosomal abnormalities in rapidly frozen 2-cell mouse embryos and the ice-forming properties of the cryoprotective solution

“…The 30 M-DMSO solution was intermediate between (Levine et al, 1963), and RNA (Crawford et al, 1971), and denatured RNA will reaggregate when the DMSO is removed (Birnboim, 1972). DMSO can also transform cell lines (Ashwood-Smith, 1985), and destabilize proteins (Fahy, 1989 (Herskovits & Harrington, 1972 (Fahy, 1989). It is also possible that the behaviour of cryoprotectant solutions exter¬ nal to the embryo are not representative of the intracellular environment.…”

DNA renaturation at the water-phenol interface

“…What is the origin of the efficiency of phenol as a single-stranded nucleic acid binding and confining compound? What are the respective roles of hydrophobicity and aromaticity in the interaction of phenol and other simple organic compounds with nucleic acids (9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)? What is the mechanism of the interfacial renaturation, with or without shaking?…”