Solution structures of the adhesion molecule DdCAD-1 reveal new insights into Ca2+-dependent cell-cell adhesion

Lin, Zhiang; Sriskanthadevan, Shrivani; Huang, Haibo; Siu, Chi‐Hung; Yang, Daiwen

doi:10.1038/nsmb1162

Cited by 29 publications

(55 citation statements)

References 34 publications

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“…The cell adhesion molecule DdCad-1 from D. discoideum is involved in cell-cell adhesion in a Ca 2ϩ -dependent manner via dimer formation through two ␤␥-crystallin domains (91). During its transport, the protein is internalized in vacuoles in a Ca 2ϩ -and conformation-dependent manner (92,93).…”

Section: ؉ Binding and Domain Stabilizationmentioning

confidence: 99%

Ca2+-binding Motif of βγ-Crystallins

Srivastava¹,

Mishra

Krishnan³

et al. 2014

Journal of Biological Chemistry

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␤␥-Crystallin-type double clamp (N/D)(N/D)XX(S/T)S motif is an established but sparsely investigated motif for Ca2؉ binding. A ␤␥-crystallin domain is formed of two Greek key motifs, accommodating two Ca 2؉ -binding sites. ␤␥-Crystallins make a separate class of Ca 2؉ -binding proteins (CaBP), apparently a major group of CaBP in bacteria. Paralleling the diversity in ␤␥-crystallin domains, these motifs also show great diversity, both in structure and in function. Although the expression of some of them has been associated with stress, virulence, and adhesion, the functional implications of Ca 2؉ binding to ␤␥-crystallins in mediating biological processes are yet to be elucidated.

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Section: ؉ Binding and Domain Stabilizationmentioning

confidence: 99%

Ca2+-binding Motif of βγ-Crystallins

Srivastava¹,

Mishra

Krishnan³

et al. 2014

Journal of Biological Chemistry

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“…Subcellular localization shows that DdCAD-1 was distributed in cytoplasm in the initial of development and is transferred to plasma membrane quickly within 3 h [3]. DdCAD-1 contains two domains, N-terminal domain has homophilic binding function, while C-terminal domain is responsible for tethering DdCAD-1 on cell membrane [4,5]. DdCAD-1 is transported to cell surface by contractile vacuoles [6], and time-lapse images show that some of DdCAD-1 is imported to contractile vacuoles by membrane invagination [7].…”

Section: Introductionmentioning

confidence: 99%

ATP-Binding Cassette Transporter B4 Anchors the Cell Adhesion Molecule DdCAD-1 to Cell Membrane in Dictyostelium discoideum

et al. 2013

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In Dictyostelium, soluble cell adhesion molecule, DdCAD-1, regulates cell-cell interaction through an unknown anchoring protein on the plasma membrane. Far western blot analysis using different probes revealed that the potential DdCAD-1 interacting protein was between 64 and 98 kDa. To isolate and identify the anchoring protein, GST-DdCAD-1 and anchoring protein were cross-linked in vivo by chemical cross-linker and stable protein complex was isolated by co-immunoprecipitation assays. The protein cross-linked to DdCAD-1 was extracted from the gel slice and trypsinized. The peptides were subjected to analysis by mass spectrometry, which showed that the putative anchoring protein belongs to ATP-binding cassette transporter family.

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“…Rescue of cadA -phenotype by in vitro reconstitution of DdCAD-1 DdCAD-1 is synthesized as a soluble protein, which adheres to the cell surface via interaction with an as yet unidentified membrane anchoring protein (Lin et al, 2006). As this membrane anchoring protein is expected to be present in cadAcells, exogenously added DdCAD-1 should bind to cadA -cells.…”

Section: Dynamic Changes In the Temporal And Spatial Distribution Of mentioning

confidence: 99%

“…DdCAD-1 contains two distinct domains with -sandwich architecture. Whereas the N-terminal domain is involved in homophilic binding, the C-terminal domain tethers the protein to a membrane anchor (Lin et al, 2006). Studies on cadA -cells have implicated DdCAD-1 in cell sorting and cell-type proportioning (Wong et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

The cell adhesion molecule DdCAD-1 regulates morphogenesis through differential spatiotemporal expression inDictyostelium discoideum

et al. 2011

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SUMMARYDuring development of Dictyostelium, multiple cell types are formed and undergo a coordinated series of morphogenetic movements guided by their adhesive properties and other cellular factors. DdCAD-1 is a unique homophilic cell adhesion molecule encoded by the cadA gene. It is synthesized in the cytoplasm and transported to the plasma membrane by contractile vacuoles. In chimeras developed on soil plates, DdCAD-1-expressing cells showed greater propensity to develop into spores than did cadA-null cells. When development was performed on non-nutrient agar, wild-type cells sorted from the cadA-null cells and moved to the anterior zone. They differentiated mostly into stalk cells and eventually died, whereas the cadA-null cells survived as spores. To assess the role of DdCAD-1 in this novel behavior of wild-type and mutant cells, cadA-null cells were rescued by the ectopic expression of DdCAD-1-GFP. Morphological studies have revealed major spatiotemporal changes in the subcellular distribution of DdCAD-1 during development. Whereas DdCAD-1 became internalized in most cells in the post-aggregation stages, it was prominent in the contact regions of anterior cells. Cell sorting was also restored in cadA -slugs by exogenous recombinant DdCAD-1. Remarkably, DdCAD-1 remained on the surface of anterior cells, whereas it was internalized in the posterior cells. Additionally, DdCAD-1-expressing cells migrated slower than cadA -cells and sorted to the anterior region of chimeric slugs. These results show that DdCAD-1 influences the sorting behavior of cells in slugs by its differential distribution on the prestalk and prespore cells.

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Solution structures of the adhesion molecule DdCAD-1 reveal new insights into Ca2+-dependent cell-cell adhesion

Cited by 29 publications

References 34 publications

Ca2+-binding Motif of βγ-Crystallins

Ca2+-binding Motif of βγ-Crystallins

ATP-Binding Cassette Transporter B4 Anchors the Cell Adhesion Molecule DdCAD-1 to Cell Membrane in Dictyostelium discoideum

The cell adhesion molecule DdCAD-1 regulates morphogenesis through differential spatiotemporal expression inDictyostelium discoideum

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