Solution Structure of the Major DNA-binding Domain of Arabidopsis thaliana Ethylene-insensitive3-like3

Yamasaki, Kazuhiko; Kigawa, T.; Inoue, Makoto; Yamasaki, Tomoko; Yabuki, Takashi; Aoki, Masaaki; Seki, Eiko; Matsuda, Takayoshi; Tomo, Yasuko; Terada, Takaho; Shirouzu, Mikako; Tanaka, Akiko; Seki, Motoaki; Shinozaki, Kazuo; Yokoyama, Shigeyuki

doi:10.1016/j.jmb.2005.02.065

Cited by 80 publications

(60 citation statements)

References 36 publications

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“…The comparison reveals a low degree of homology (Z-score <2.0) with two protein fragments holding a-helices with similar relative orientation as in CtD-Ole e 9. One of them, a DNA binding domain of Arabidopsis thaliana ethylene-insensitive3-like3 (Yamasaki et al 2005), shows a reasonable superposition with CtD-Ole e 9.…”

Section: Resultsmentioning

confidence: 85%

Solution structure of the C‐terminal domain of Ole e 9, a major allergen of olive pollen

et al. 2008

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Ole e 9 is an olive pollen allergen belonging to group 2 of pathogenesis-related proteins. The protein is composed of two immunological independent domains: an N-terminal domain (NtD) with 1,3-bglucanase activity, and a C-terminal domain (CtD) that binds 1,3-b-glucans. We have determined the three-dimensional structure of CtD-Ole e 9 (101 amino acids), which consists of two parallel a-helices forming an angle of ;55°, a small antiparallel b-sheet with two short strands, and a 3-10 helix turn, all connected by long coil segments, resembling a novel type of folding among allergens. Two regions surrounded by aromatic residues (F49, Y60, F96, Y91 and Y31, H68, Y65, F78) have been localized on the protein surface, and a role for sugar binding is suggested. The epitope mapping of CtD-Ole e 9 shows that B-cell epitopes are mainly located on loops, although some of them are contained in secondary structural elements. Interestingly, the IgG and IgE epitopes are contiguous or overlapped, rather than coincident. The three-dimensional structure of CtD-Ole e 9 might help to understand the underlying mechanism of its biochemical function and to determine possible structure-allergenicity relationships.

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Section: Resultsmentioning

confidence: 85%

Solution structure of the C‐terminal domain of Ole e 9, a major allergen of olive pollen

et al. 2008

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“…Together, EIN3 and EIL1 cooperatively and differentially regulate the full array of ethylene responses, with EIN3 mainly controlling seedling responses and EIL1 having a greater role in adult leaves and stems . EIN3/EIL1 activate a transcriptional cascade by binding as homodimers (Solano et al, 1998;Yamasaki et al, 2005) to the promoters of ERF genes, such as ERF1 (Solano et al, 1998), ETHYLENE AND SALT INDUCIBLE1 (Zhang et al, 2011), and other ERF genes (Chang et al, 2013;Figs. 2 and 3A) in the APETALA2-ETHYLENE RESPONSE ELEMENT BINDING PROTEIN transcription factor family.…”

Section: The Ein3/eil1 Transcription Factors Initiate a Transcriptionmentioning

confidence: 99%

Mechanistic Insights in Ethylene Perception and Signal Transduction

2015

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The gaseous hormone ethylene profoundly affects plant growth, development, and stress responses. Ethylene perception occurs at the endoplasmic reticulum membrane, and signal transduction leads to a transcriptional cascade that initiates diverse responses, often in conjunction with other signals. Recent findings provide a more complete picture of the components and mechanisms in ethylene signaling, now rendering a more dynamic view of this conserved pathway. This includes newly identified protein-protein interactions at the endoplasmic reticulum membrane, as well as the major discoveries that the central regulator ETHYLENE INSENSITIVE2 (EIN2) is the long-sought phosphorylation substrate for the CONSTITUTIVE RESPONSE1 protein kinase, and that cleavage of EIN2 transmits the signal to the nucleus. In the nucleus, hundreds of potential gene targets of the EIN3 master transcription factor have been identified and found to be induced in transcriptional waves, and transcriptional coregulation has been shown to be a mechanism of ethylene cross talk.

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“…In the absence of ethylene, EBFs are up-regulated and mediate EIN3 degradation (Solano et al, 1998). EIL TFs act on the promoters of early ethylene responsive genes such as ETHYLENE-RESPONSE-FACTOR1 (ERF1), which encodes a GCCbox-binding protein, by binding to the primary ethylene response element in the promoters of these genes (Yamasaki et al, 2005). EILs in kiwifruit were able to activate the 1-aminocyclopropane-1-carboxylic acid (ACC) OXIDASE1 (AdACO1) gene promoter, thus implicating them in a role during autocatalytic ethylene production (Yin et al, 2010).…”

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confidence: 99%

Natural Variation in Monoterpene Synthesis in Kiwifruit: Transcriptional Regulation of Terpene Synthases by NAC and ETHYLENE-INSENSITIVE3-Like Transcription Factors

et al. 2015

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Two kiwifruit (Actinidia) species with contrasting terpene profiles were compared to understand the regulation of fruit monoterpene production. High rates of terpinolene production in ripe Actinidia arguta fruit were correlated with increasing gene and protein expression of A. arguta terpene synthase1 (AaTPS1) and correlated with an increase in transcript levels of the 2-C-methyl-D-erythritol 4-phosphate pathway enzyme 1-deoxy-D-xylulose-5-phosphate synthase (DXS). Actinidia chinensis terpene synthase1 (AcTPS1) was identified as part of an array of eight tandemly duplicated genes, and AcTPS1 expression and terpene production were observed only at low levels in developing fruit. Transient overexpression of DXS in Nicotiana benthamiana leaves elevated monoterpene synthesis by AaTPS1 more than 100-fold, indicating that DXS is likely to be the key step in regulating 2-C-methyl-D-erythritol 4-phosphate substrate flux in kiwifruit. Comparative promoter analysis identified potential NAC (for no apical meristem [NAM], Arabidopsis transcription activation factor [ATAF], and cup-shaped cotyledon [CUC])-domain transcription factor) and ETHYLENE-INSENSITIVE3-like transcription factor (TF) binding sites in the AaTPS1promoter, and cloned members of both TF classes were able to activate the AaTPS1 promoter in transient assays. Electrophoretic mobility shift assays showed that AaNAC2, AaNAC3, and AaNAC4 bind a 28-bp fragment of the proximal NAC binding site in the AaTPS1 promoter but not the A. chinensis AcTPS1 promoter, where the NAC binding site was mutated. Activation could be restored by reintroducing multiple repeats of the 12-bp NAC core-binding motif. The absence of NAC transcriptional activation in ripe A. chinensis fruit can account for the low accumulation of AcTPS1 transcript, protein, and monoterpene volatiles in this species. These results indicate the importance of NAC TFs in controlling monoterpene production and other traits in ripening fruits.

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Solution Structure of the Major DNA-binding Domain of Arabidopsis thaliana Ethylene-insensitive3-like3

Cited by 80 publications

References 36 publications

Solution structure of the C‐terminal domain of Ole e 9, a major allergen of olive pollen

Solution structure of the C‐terminal domain of Ole e 9, a major allergen of olive pollen

Mechanistic Insights in Ethylene Perception and Signal Transduction

Natural Variation in Monoterpene Synthesis in Kiwifruit: Transcriptional Regulation of Terpene Synthases by NAC and ETHYLENE-INSENSITIVE3-Like Transcription Factors

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