Solution Stability of Poloxamer 188 Under Stress Conditions

Wang, Tingting; Markham, Aaron P.; Thomas, Steven J.; Wang, Ning; Huang, Lihua; Clemens, Matthew; Rajagopalan, Natarajan

doi:10.1016/j.xphs.2018.10.057

Cited by 38 publications

(18 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recent study confirms that P188 can keep good chemical stability in solution under environment stress such as temperature and pH alteration [ 25 ]. Moreover, P188 is also a novel reagent of biomaterials without impairing biofunction and activity [ 22 , 23 , 24 , 25 ]. Several studies have showed that P188 can help resist peroxidation of membrane and prevent damages of cytoskeleton and apoptosis [ 22 , 23 , 24 ].…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, P188 is also a novel reagent of biomaterials without impairing biofunction and activity [ 22 , 23 , 24 , 25 ]. Several studies have showed that P188 can help resist peroxidation of membrane and prevent damages of cytoskeleton and apoptosis [ 22 , 23 , 24 ]. However, very few studies have investigated the effect of P188 on immune response.…”

Section: Discussionmentioning

confidence: 99%

“…P188 shows excellent solubility, clarity, and concentration-dependent viscosity in aqueous solution [ 21 ]. When used as a pharmaceutical excipient, P188 exhibits lower cytotoxicity because of its ability to protect cells against peroxidation of membrane, damages of cytoskeleton, and apoptosis [ 22 , 23 , 24 , 25 ]. In addition, a study also demonstrated that P188 can provide neuron protection by inhibiting p38 mitogen-activated protein kinase (p38MAPK) [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Poloxamer-188 Adjuvant Efficiently Maintains Adaptive Immunity of SARS-CoV-2 RBD Subunit Vaccination through Repressing p38MAPK Signaling

et al. 2022

View full text Add to dashboard Cite

Poloxamer-188 (P188) is a nonionic triblock linear copolymer that can be used as a pharmaceutical excipient because of its amphiphilic nature. This study investigated whether P188 can act as an adjuvant to improve the immunogenicity of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) receptor binding domain (RBD) subunit vaccine. BALB/c mice were vaccinated twice with the RBD antigen alone or in combination with P188 or MF59 (a commercial adjuvant for comparison purposes). The resulting humoral and cellular immunity were assessed. Results showed that P188 helped elicit higher neutralizing activity than MF59 after vaccination. P188 induced significant humoral immune response, along with type 1 T helper (Th1) and type 2 T helper (Th2) cellular immune response when compared with MF59 due to repressing p38MAPK phosphorylation. Furthermore, P188 did not result in adverse effects such as fibrosis of liver or kidney after vaccination. In conclusion, P188 is a novel adjuvant that may be used for safe and effective immune enhancement of the SARS-CoV-2 RBD antigen.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Poloxamer-188 Adjuvant Efficiently Maintains Adaptive Immunity of SARS-CoV-2 RBD Subunit Vaccination through Repressing p38MAPK Signaling

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Costa et al (1996) found small molecules, such as formaldehyde, acetaldehyde, and formic acid as degradation products [443]. The volatile products of degradation can be monitored by using GC-MS [444,445]. Since there are few detection options described in literature to detect poloxamer oxidation, a decrease in concentration can be used.…”

Section: Photo-oxidation Of Excipientsmentioning

confidence: 99%

Photo-Oxidation of Therapeutic Protein Formulations: From Radical Formation to Analytical Techniques

et al. 2021

View full text Add to dashboard Cite

UV and ambient light-induced modifications and related degradation of therapeutic proteins are observed during manufacturing and storage. Therefore, to ensure product quality, protein formulations need to be analyzed with respect to photo-degradation processes and eventually protected from light exposure. This task usually demands the application and combination of various analytical methods. This review addresses analytical aspects of investigating photo-oxidation products and related mediators such as reactive oxygen species generated via UV and ambient light with well-established and novel techniques.

show abstract

“…Regarding their stability, poloxamers have been shown to degrade through the mechanisms of autooxidation and chain scission involving free radicals [ 97 ].…”

Section: Excipients Used In Ocular Formulations For Modified Drug Deliverymentioning

confidence: 99%

Recent Advances in the Excipients Used for Modified Ocular Drug Delivery

et al. 2021

View full text Add to dashboard Cite

In ocular drug delivery, maintaining an efficient concentration of the drug in the target area for a sufficient period of time is a challenging task. There is a pressing need for the development of effective strategies for drug delivery to the eye using recent advances in material sciences and novel approaches to drug delivery. This review summarizes the important aspects of ocular drug delivery and the factors affecting drug absorption in the eye including encapsulating excipients (chitosan, hyaluronic acid, poloxamer, PLGA, PVCL-PVA-PEG, cetalkonium chloride, and gelatin) for modified drug delivery.

show abstract

Solution Stability of Poloxamer 188 Under Stress Conditions

Cited by 38 publications

References 19 publications

Poloxamer-188 Adjuvant Efficiently Maintains Adaptive Immunity of SARS-CoV-2 RBD Subunit Vaccination through Repressing p38MAPK Signaling

Poloxamer-188 Adjuvant Efficiently Maintains Adaptive Immunity of SARS-CoV-2 RBD Subunit Vaccination through Repressing p38MAPK Signaling

Photo-Oxidation of Therapeutic Protein Formulations: From Radical Formation to Analytical Techniques

Recent Advances in the Excipients Used for Modified Ocular Drug Delivery

Contact Info

Product

Resources

About