Solution properties of viilian, the exopolysaccharide fromLactococcus lactis subsp.cremoris SBT 0495

Higashimura, M.; Mulder-Bosman, B. W.; Reich, R.; Iwasaki, Taisuke; Robijn, Gerard W.

doi:10.1002/1097-0282(200008)54:2<143::aid-bip7>3.0.co;2-q

Cited by 34 publications

(6 citation statements)

References 33 publications

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“…Several authors have used theoretical models to estimate the electrostatic contribution to the persistence lengths, most commonly on the basis of R G -M data, but also from [η]-M data. 27,41 As an alternative, we introduce here another, quite simple approach, taking advantage of the empirically observed linear relationship between the intrinsic viscosity and the I 1/2 through B parameters 10 The B-parameter and the exponent ν (usually set to 1.3) are known for a series of alginates, 8,10 including periodate-oxidized alginates. 21 We used this relation to extrapolate our [η] values obtained by SMV (for each elution slice) to infinite ionic strength ([η] ∞ ).…”

Section: Discussionmentioning

confidence: 99%

A Study of the Chain Stiffness and Extension of Alginates, in Vitro Epimerized Alginates, and Periodate-Oxidized Alginates Using Size-Exclusion Chromatography Combined with Light Scattering and Viscosity Detectors

2006

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A series of alginates isolated from the stem and leaf of a brown algae (Laminaria hyperborea), bacterial mannuronan, in vitro epimerized mannuronans, and periodate oxidized alginates were analyzed by size-exclusion chromatography (SEC) combined with online multiangle laser light scattering (MALS) and viscometry (collectively abbreviated SMV). Selected samples were also analyzed off-line using low-angle laser light scattering and capillary viscometry. Excellent agreement between the two methods was obtained for properly purified samples. In contrast, abnormal results were obtained for some industrial samples due to the presence of particulate material. Naturally occurring alginates and in vitro epimerized mannuronans were found to obey essentially the same RG-M and [eta]-M relations, and hence, the same Mark-Houwink-Sakurada (MHS) equations (valid for I = 0.10 M): 20 000 g/mol < M < 100 000 g/mol, [eta] = 0.0054 .M(1.00); 100 000 g/mol < M < 1 000 000 g/mol, [eta] = 0.071 .M(0.89). Application of the wormlike chain model to the [eta]-M data obtained by SMV yielded persistence lengths (q) of 15 nm for all alginates at an ionic strength of 0.17 M. Intrinsic viscosities corresponding to infinite ionic strength were estimated on the basis of Smidsrød's B-parameter, and the wormlike chain model then yielded q = 12 nm. Periodate oxidized alginates showed, in contrast, a pronounced decrease in persistence length with increasing degree of oxidation, reaching values below 4 nm at 44% oxidation. Periodate oxidation also resulted in some depolymerization, even in the presence of a free-radical scavenger.

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Section: Discussionmentioning

confidence: 99%

A Study of the Chain Stiffness and Extension of Alginates, in Vitro Epimerized Alginates, and Periodate-Oxidized Alginates Using Size-Exclusion Chromatography Combined with Light Scattering and Viscosity Detectors

2006

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“…The result of the characterization of some Astragalus gum exudates indicated that intrinsic viscosity of soluble part of different species is quite different [13]. Nowadays, GPC combined with laser light scattering has been demonstrated to be a very powerful absolute method for characterization of polysaccharides [28][29][30].…”

Section: Introductionmentioning

confidence: 99%

Solution properties of targacanthin (water-soluble part of gum tragacanth exudate from Astragalus gossypinus)

Mohammadifar

Musavi

Kiumarsi³

et al. 2006

International Journal of Biological Macromolecules

192

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“…cremoris SBT 0495 has, however, been subjected to a through physico-chemical study (Higashimura, Mulder-Bosman, Reich, Iwasaki & Robijn, 2000) using several methods. Viilian 312 has a pentasaccharide repeating unit where the main chain part consists of the trisaccharide →4)-313 β-D-Glcp-(1→4)-β-D-Galp-(1→4)-β-D-Glcp-(1→.…”

Section: Results 136mentioning

confidence: 99%

The size and shape of three water-soluble, non-ionic polysaccharides produced by lactic acid bacteria: A comparative study

Dalheim

Arnfinnsdottir

Widmalm

et al. 2016

Carbohydrate Polymers

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Three water-soluble, non-ionic extracellular polysaccharides (EPS) obtained from lactic acid bacteria (S. thermophilus THS, L. helveticus K16 and S. thermophilus ST1) were subjected to a comparative study by means of multidetector size-exclusion chromatography, providing distributions and averages of molar masses, radii of gyration and intrinsic viscosities. All polysaccharides displayed random coil character. Further analysis of the data reveals differences in chain stiffness and extension that could be well correlated to structural features. The calculated persistence lengths ranged from 5 to 10nm and fall within the range typical for many single-stranded bacterial or plant polysaccharides. The ST1 polysaccharide had the highest molar mass but the lowest persistence length, which is attributed to the presence of the flexible (1→6)-linkage in the main chain.

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Solution properties of viilian, the exopolysaccharide fromLactococcus lactis subsp.cremoris SBT 0495

Cited by 34 publications

References 33 publications

A Study of the Chain Stiffness and Extension of Alginates, in Vitro Epimerized Alginates, and Periodate-Oxidized Alginates Using Size-Exclusion Chromatography Combined with Light Scattering and Viscosity Detectors

A Study of the Chain Stiffness and Extension of Alginates, in Vitro Epimerized Alginates, and Periodate-Oxidized Alginates Using Size-Exclusion Chromatography Combined with Light Scattering and Viscosity Detectors

Solution properties of targacanthin (water-soluble part of gum tragacanth exudate from Astragalus gossypinus)

The size and shape of three water-soluble, non-ionic polysaccharides produced by lactic acid bacteria: A comparative study

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