The mean-square radius of gyration 〈S 2 〉 and second virial coefficient A 2 are determined from static light scattering (LS) measurements for six samples of poly(di-tert-butyl fumarate) (PDtBF) for a range of weight-average molecular weight M w from 4.35 × 10 4 to 1.73 × 10 5 in tetrahydrofuran at 30.0°C. The intrinsic viscosity [η] and translational diffusion coefficient D are also determined from viscosity and dynamic LS measurements for those samples under the same solvent conditions. From a simultaneous analysis of 〈S 2 〉 and A 2 based on the Kratky-Porod (KP) wormlike chain with excluded volume, the stiffness parameter λ − 1 and the reduced excluded-volume strength λB are determined to be 350 Å and 0.024, respectively, where we assume the shift factor M L for PDtBF to be 101 Å − 1 on the analogy of the main-chain structure of poly(diisopropyl fumarate) previously studied. For PDtBF with such a large λ − 1 , it is found that the intramolecular excluded-volume effect for 〈S 2 〉 is negligibly small in the range of M w examined. As for [η] and D, their behavior may be well explained by the corresponding KP theories using the model parameter values consistent with those determined from 〈S 2 〉 and A 2 , and the intramolecular excludedvolume effect for them can be ignored, as in the case of 〈S 2 〉.