Solution phase photophysics of 5,7-dimethoxy-2,3,4,9-tetrahydro-1H-carbazol-1-one: Analysing the lineaments of a new fluorosensor to probe different micro-environments

Constrained Photophysics of 5,7-dimethoxy-2,3,4,9-tetrahydro-1H-carbazol-1-one in the Bioenvironment of Serum Albumins: A Spectroscopic Endeavour Supported by Molecular Docking Analysis

“…Our fluorophore of interest KTHC-57 has been synthesized and purified following a literature procedure and is described elsewhere [49]. Phosphate buffer solution (pH~7 and ionic strength =0.15 M) has been used as the buffer solution.…”

“…Before moving into the details of confined photophysics of KTHC-57 within serum albumin confinement, it is relevant to cast light on its behaviour in homogeneous liquid so that we can appreciate the modulations to its photophysical properties in the confined state of serum albumins [49]. Enhancement of dipole moment of KTHC-57, a reporter of its immediate microenvironment, had been observed to be 11.72 D and 6.11 D in the case of protic and aprotic solvents respectively [49].…”

“…Enhancement of dipole moment of KTHC-57, a reporter of its immediate microenvironment, had been observed to be 11.72 D and 6.11 D in the case of protic and aprotic solvents respectively [49]. Excited state spectral features had been chiefly guided by two parameters namely, hydrogen bond donor acidity of the solvent and solvent dipolarity [49]. Even in the presence of different binary mixtures, appreciable changes in emission spectrum of KTHC-57 had been observed.…”

“…Even in the presence of different binary mixtures, appreciable changes in emission spectrum of KTHC-57 had been observed. Alterations in photophysical responses of KTHC-57 in different organized assemblies always had been noteworthy [49]. Considerable hypsochromic shift along with an enhancement of intensity had been the indication of entrapment of fluorophore in the hydrophobic regions of organised assemblies [49].…”

“…The dye has been used extensively for various photophysical applications as described elsewhere [49]. In scientific literature, there is no scarcity of examples of various fluorophore-BSA ⁄ HSA binding interactions [11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28].…”