Solution-Phase Library Screening for Identification of Rare Adrenergic Receptor Clones

Noradrenaline contractions of human prostate mediated by α1c‐(α1c‐) adrenoceptor subtype

“…Chlorethylclonidine selectively alkylates tissue 2lB-adrenoceptors (e.g. in rat spleen, Burt et al, 1995) and the expressed Ilb-adrenoceptor clone (Perez et al, 1991) rather than tissue IIA-adrenoceptors (e.g. lack of effect in rat vas deferens, Burt et al, 1995) or the expressed alc-and ald-adrenoceptor clones (Forray et al, 1994a;Perez et al, 1991).…”

“…All three subtypes have been cloned from both rat and human cDNA libraries (Lomasney et al, 1991;Bruno et al, 1991;Ramarao et al, 1992;Laz et al, 1993;Hirasawa et al, 1993) and are expressed tissue-dependently in both species (Rokosh et al, 1994;Price et al, 1994 (Perez et al, 1991). Schwinn & Lomasney (1992) Perez et al (1991), and also had a different pharmacology from the a1A subtype. There is currently little evidence for functional alD-adrenoceptors although they may be present in the rat vasculature (Saussy et al, 1994).…”

“…There is currently little evidence for functional alD-adrenoceptors although they may be present in the rat vasculature (Saussy et al, 1994). The alc clone is now considered to represent the classical a1A subtype found in tissues (Laz et al, 1993;Perez et al, 1994;Burt et al, 1995). The al clone was originally isolated from a bovine brain cDNA library and although it had a very similar pharmacology to the OClA-adrenoceptor, it was thought to represent a new subtype as it was also chlorethylclonidine-sensitive (Schwinn et al, 1990).…”

Mice expressing the α1B‐adrenergic receptor induces a synucleinopathy with excessive tyrosine nitration but decreased phosphorylation

“…The three a 1 -AR subtypes (a 1A , a 1B , a 1D ) have been classified via molecular cloning (Cotecchia et al 1988;Lomasney et al 1991;Perez et al 1991;Perez et al 1994). The most well characterized cardiovascular functions associated with a 1 AR stimulation include the contraction and growth of vascular smooth muscle cells and their regulation of blood pressure (Chen et al 1995;Leech and Faber 1996;Cavalli et al 1997;Hrometz et al 1999).…”

Pharmacological Characterization of Contractile Responses Induced by .ALPHA.1-Agonists, Norepinephrine and Clonidine, by Selective Antagonists of Their Subtypes in Rabbit Thoracic Aorta.

“…Muramatsu et al (15) proposed that a 1-adrenoceptors in blood vessels can be divided into three subtypes des ignated as a 1H, a 1L, and a IN by their antagonist affin ity and susceptibility to chloroethylclonidine or nifedi pine. Molecular cloning studies have also revealed heterogeneity in a 1-adrenoceptors (16)(17)(18)(19). In the pul monary arteries of rabbits and dogs, Holck et al (20) and Flavahan et al (21) showed that prazosin antago nized the contractile responses to methoxamine, cloni dine and phenylephrine with two different affinities.…”