Solution-Deposited Thin Silver Films on Plastic Surfaces for Low-Cost Applications in Plasmon-Coupled Emission Sensors

Sathish, R. Sai; Kostov, Yordan; Smith, Derek; Rao, Govind

doi:10.1007/s11468-009-9083-5

Cited by 11 publications

(9 citation statements)

References 23 publications

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“…2 In this regard, signal enhancement due to efficient fluorescence−plasmon coupling using index matching fluids which also result in a uniform refractive index medium for the light to pass through were explored. 15 In addition, the use of incident light whose energy matched the excitation maximum of the fluorophore has also been explored, and notable progress toward the development of low-cost sensing platforms has been reported. 16 Lastly, it should be mentioned that the use of specially engineered conical mirrors for efficient signal collection can further enhance the performance of SPCE sensors, albeit the associated high cost restricts its use in highly specialized applications.…”

Section: Introductionmentioning

confidence: 99%

Amplification of Surface Plasmon Coupled Emission from Graphene–Ag Hybrid Films

Mulpur

Podila²,

Lingam³

et al. 2013

J. Phys. Chem. C

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Surface plasmon coupled emission (SPCE) is a novel analytical technique in which the isotropic emission of a fluorophore is combined with the surface plasmon resonance of a Ag (or Au) thin film to yield highly directional emission from the so-called plasmaphore and thus greatly increased sensitivity. Here we report a 40-fold amplification of rhodamine B (RhB) fluorophore when graphene is used as the spacer layer in a conventional Ag-based SPCE setup. In addition to protecting the Ag thin film from oxidation, the highly impermeable graphene facilitated π–π stacking interactions with the RhB molecules that resulted in an efficient radiated emission from the plasmaphore. In addition, we found that the plasmaphore emission was more sensitively dependent on the in-plane crystallinity (measured by coherence length L a) of the graphene spacer layer than its thickness. This study describes the unique features of graphene as a spacer layer for SPCE-based analytical platforms and its potential applications in chem-bio sensing and detection.

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Section: Introductionmentioning

confidence: 99%

Amplification of Surface Plasmon Coupled Emission from Graphene–Ag Hybrid Films

Mulpur

Podila²,

Lingam³

et al. 2013

J. Phys. Chem. C

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“…We were able to produce different device designs, such as T-/Y-channel mixers, single channels capable of bearing a fluid path for surface plasmon-coupled enhanced (SPCE) fluorescence and detection of microbial contamination using resazurin. A primary focus for this manuscript was to demonstrate the use of POC wood chips for protein detection via SPCE. − SPCE has gained a lot of attention in the past decade because of its ability to address inherent drawbacks of conventional fluorescence particularly when the fluorophore has a low quantum yield. Fluorescence based detection has hugely impacted the current generation of biomedical, diagnostic and sensing devices .…”

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confidence: 99%

Wood Microfluidics

et al. 2019

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As nonbiodegradable plastics continue to pollute our land and oceans, countries are starting to ban the use of single-use plastics. In this paper, we demonstrated the fabrication of wood-based microfluidic devices and their adaptability for single-use, point-of-care (POC) applications. These devices are made from easily sourced renewable materials for fabrication while exhibiting all the advantages of plastic devices without the problem of nonbiodegradable waste and cost. To build these wood devices, we utilized laser engraving and traditional mechanical methods and have adapted specific surface coatings to counter the wicking effect of wood. To demonstrate their versatility, wood microfluidic devices were adapted for (i) surface plasmon coupled enhancement (SPCE) of fluorescence for detection of proteins, (ii) T-/Y-geometry microfluidic channel mixers, and (iii) devices for rapid detection of microbial contamination. These provide proof of concept for the use of wooden platforms for POC applications. In this study, we measured the fluorescence intensities of recombinant green fluorescent protein (GFP) standards (ranging from 1.5−25 ng/μL) and 6XHis-G-CSF (ranging from 0.1−100 ng/μL) expressed in cell-free translation systems. All tested devices perform as well as or better than their plastic counterparts.

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“…Ground-state molecular aggregation due to the proximity among dye molecules on labeled proteins results in a congested spectrum that is difficult to interpret using conventional fluorescence spectroscopy. We exploit the use of SPCE to this end to study the photophysics of protein monolayers SPCE substrates obtained by solution deposition [20,21]. However, it is known that direct positioning of a fluorophore within 5 nm of a metal surface results in significant fluorescence quenching.…”

Section: Resultsmentioning

confidence: 99%

“…A detailed low-cost procedure for the solution-based deposition achieved by optimizing the deposition time and temperature has been recently described by us [20,21]. The silvered substrates were subsequently spin-coated with a 15-nm-thick film of poly(methyl methacrylate) (PMMA) (2 mg/mL in chloroform) at 1,000 rpm for 30 s, and then the films were allowed to dry for another 30 s [22,23].…”

Section: Methodsmentioning

confidence: 99%

Studies of Surface-Adsorbed Fluorescently Labeled Casein and Concanavalin A Using Surface Plasmon-Coupled Emission

2010

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We report the use of surface plasmon-coupled emission (SPCE) as an analytical tool to study the photophysics of surface-adsorbed fluorescently labeled proteins. The study uses plasma etching of PMMA surface followed by deposition of poly(diallyldimethylammonium chloride) (PDDA) for surface protein detection. PDDA increases the overall amount of the captured protein and also promotes dye aggregation. The photon-sorting properties of the SPCE process allows for wavelength separation of the individual components from the protein-dye aggregates. This has been exploited to study the fluorescence emissions from casein labeled with fluorescein isothiocyanate and concanavalin A labeled with tetramethylrhodamine. Based on the current findings, the proteins can be used to measure background fluorescence or to monitor the microenvironments in fluoroimmunoassays on SPCE substrates.

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Solution-Deposited Thin Silver Films on Plastic Surfaces for Low-Cost Applications in Plasmon-Coupled Emission Sensors

Cited by 11 publications

References 23 publications

Amplification of Surface Plasmon Coupled Emission from Graphene–Ag Hybrid Films

Amplification of Surface Plasmon Coupled Emission from Graphene–Ag Hybrid Films

Wood Microfluidics

Studies of Surface-Adsorbed Fluorescently Labeled Casein and Concanavalin A Using Surface Plasmon-Coupled Emission

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