Solution-blown core–shell hydrogel nanofibers for bovine serum albumin affinity adsorption

Tong, Junying; Xu, Xin; Wang, Hang; Zhuang, Xupin; Zhang, Fang

doi:10.1039/c5ra19420b

Cited by 17 publications

(8 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hydration of glutaraldehyde crosslinked collagen scaffolds in culture significantly decreased mechanical properties, reducing its potential for broader clinical applications such as meniscus replacement. Incorporation of artificial polymers, such as PLA, may increase the mechanical properties as we and others have previously demonstrated with coaxial electrospun fibers (Tong et al, 2015;Singh et al, 2018;Baek et al, 2019;Baek et al, 2020). Our nozzle to collector arrangement did generate encouraging differences in mechanical properties based on sample orientation but did not result in visible fiber alignment; and alternate approaches need to be explored.…”

Section: Discussionmentioning

confidence: 75%

Pneumatospinning Biomimetic Scaffolds for Meniscus Tissue Engineering

Dorthé

Williams²,

Grogan

et al. 2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Nanofibrous scaffolds fabricated via electrospinning have been proposed for meniscus tissue regeneration. However, the electrospinning process is slow, and can only generate scaffolds of limited thickness with densely packed fibers, which limits cell distribution within the scaffold. In this study, we explored whether pneumatospinning could produce thicker collagen type I fibrous scaffolds with higher porosity, that can support cell infiltration and neo-fibrocartilage tissue formation for meniscus tissue engineering. We pneumatospun scaffolds with solutions of collagen type I with thicknesses of approximately 1 mm in 2 h. Scanning electron microscopy revealed a mix of fiber sizes with diameters ranging from 1 to 30 µm. The collagen scaffold porosity was approximately 48% with pores ranging from 7.4 to 100.7 µm. The elastic modulus of glutaraldehyde crosslinked collagen scaffolds was approximately 45 MPa, when dry, which reduced after hydration to 0.1 MPa. Mesenchymal stem cells obtained from the infrapatellar fat pad were seeded in the scaffold with high viability (>70%). Scaffolds seeded with adipose-derived stem cells and cultured for 3 weeks exhibited a fibrocartilage meniscus-like phenotype (expressing COL1A1, COL2A1 and COMP). Ex vivo implantation in healthy bovine and arthritic human meniscal explants resulted in the development of fibrocartilage-like neotissues that integrated with the host tissue with deposition of glycosaminoglycans and collagens type I and II. Our proof-of-concept study indicates that pneumatospinning is a promising approach to produce thicker biomimetic scaffolds more efficiently that electrospinning, and with a porosity that supports cell growth and neo-tissue formation using a clinically relevant cell source.

show abstract

Section: Discussionmentioning

confidence: 75%

Pneumatospinning Biomimetic Scaffolds for Meniscus Tissue Engineering

Dorthé

Williams²,

Grogan

et al. 2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…Synthetic polymer nanofibers are easy to spin via this process. Solution‐blown nylon 6 nanofibers have been developed as highly effective air filtration media and protective materials . However, nature polymers are relatively difficult to blow to fibers because of their high viscosity of their solution even in low concentration.…”

Section: Resultsmentioning

confidence: 99%

“…Cibacron blue F3GA was covalently coupled to the nanofibers via nucleophilic reaction between the chloro groups of its triazine ring and the amino groups of CS shell at alkaline conditions . CB F3GA (50 mg) was dissolved in 100 ml of water.…”

Section: Methodsmentioning

confidence: 99%

Solution-blown nylon 6-chitosan core-shell nanofiber for highly efficient affinity adsorption

Wang

Zhao

et al. 2016

Polym. Adv. Technol.

View full text Add to dashboard Cite

A facile spinning‐based strategy was developed to fabricate chitosan (CS) surface nanofiber‐based affinity membranes for protein adsorption. The core–shell nanofiber mat of nylon 6–CS was prepared via coaxial solution blowing process. The nanofibers have a diameter range of 60–300 nm. The core–shell structure was confirmed by transmission electron microscopy, and CS was observed as a thin layer that uniformly adhered to the core. The dye ligand of cibacron blue F3GA (CB F3GA) was further covalently immobilized on the nanofibers with a content of 425 µmol/g. The pristine and CB F3GA‐attached mats were studied in protein adsorption. High bovine serum albumin adsorption capacities of 91.9 and 219.6 mg/g were obtained for pristine and CB F3GA‐attached mats, respectively. Given its properties of high flux rate and low pressure drop, CB F3GA‐attached nylon 6–CS nanofiber mat meets the requirements of highly effective affinity membrane chromatography. Copyright © 2016 John Wiley & Sons, Ltd.

show abstract

“…Recent developments include multinozzle systems for industrial-scale production, 119 aqueous (heated) spinning, 118 and coaxial nozzles for core−shell fiber production. 120,121 Since its first report in 2009, 27 there has been an increasing number of papers concerned with several aspects (materials and process) of solution blow spinning. Compared with electrospinning, SBS presents some advantages including no need of applying an electrical voltage, low cost; ease of fabrication and scaling 3D structures with larger mesh size.…”

Section: Solution Blowmentioning

confidence: 99%

Advances in Functional Polymer Nanofibers: From Spinning Fabrication Techniques to Recent Biomedical Applications

Santos

Corrêa

Medeiros

et al. 2020

ACS Appl. Mater. Interfaces

168

148

View full text Add to dashboard Cite

Functional polymeric micro-/nanofibers have emerged as promising materials for the construction of structures potentially useful in biomedical fields. Among all kinds of technologies to produce polymer fibers, spinning methods have gained considerable attention. Herein, we provide a recent review on advances in the design of micro-and nanofibrous platforms via spinning techniques for biomedical applications. Specifically, we emphasize electrospinning, solution blow spinning, centrifugal spinning, and microfluidic spinning approaches. We first introduce the fundamentals of these spinning methods and then highlight the potential biomedical applications of such micro-and nanostructured fibers for drug delivery, tissue engineering, regenerative medicine, disease modeling, and sensing/biosensing. Finally, we outline the current challenges and future perspectives of spinning techniques for the practical applications of polymer fibers in the biomedical field.

show abstract

Solution-blown core–shell hydrogel nanofibers for bovine serum albumin affinity adsorption

Abstract: In this work, nylon 6 core–chitosan/poly(vinyl alcohol) (PVA) shell hydrogel nanofibers (NCNFs) were fabricated by coaxial solution blowing for BSA adsorbing.

Cited by 17 publications

References 33 publications

Pneumatospinning Biomimetic Scaffolds for Meniscus Tissue Engineering

Pneumatospinning Biomimetic Scaffolds for Meniscus Tissue Engineering

Solution-blown nylon 6-chitosan core-shell nanofiber for highly efficient affinity adsorption

Advances in Functional Polymer Nanofibers: From Spinning Fabrication Techniques to Recent Biomedical Applications

Contact Info

Product

Resources

About