Soluble Sugars and Sucrose-Metabolizing Enzymes Related to Cold Acclimation of Sweet Cherry Cultivars Grafted on Different Rootstocks

Turhan, Ece; Ergin, Sergül

doi:10.1100/2012/979682

Cited by 25 publications

(13 citation statements)

References 40 publications

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“…In heterologous bitter gourd-grafted seedlings, the increase in leaf SPS activity promoted sucrose accumulation; SS and NI were conducive to the accumulation of glucose and fructose, while AI was inferior to the first three enzyme activities. This is similar to the results of Shahid et al (2018), but in a study by Turhan and Ergin (2012), stress resistance was found to increase with the reduction of NI and SS activities in the bark tissue of 4-year-old sweet cherry-grafted trees. This phenomenon largely depends on where the samples are taken from and where the carbon is assimilated.…”

Section: Discussionsupporting

confidence: 86%

Grafting promoted antioxidant capacity and carbon and nitrogen metabolism of bitter gourd seedlings under heat stress

Wen

Lian³

et al. 2022

Front. Plant Sci.

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IntroductionHeat stress can limit vegetable growth, and this can lead to constraints on agricultural production. Grafting technologies, however, can be used to alleviate various plant stresses.MethodsIn this study, the differences in the heat stress impacts and recovery abilities of pumpkin and luffa rootstocks for bitter gourd were analyzed in terms of their antioxidant activity and carbon and nitrogen metabolism.ResultsCompared with the un-grafted and self-grafted bitter gourd, which suffered from heat stress at 40°C for 24 h, heterologously grafted bitter gourd showed higher heat stability of the cell membrane (relative conductivity and malondialdehyde content were reduced), reduced oxidative stress (antioxidant enzyme activity was increased and the reactive oxygen species content reduced), and increased enzyme activity (sucrose phosphate synthase, sucrose synthase, neutral invertase, and acid invertase) and sugar content (soluble sugar, sucrose, fructose, and glucose) in carbon metabolism. The enzyme activity (nitrate reductase, nitrite reductase, and glutamine synthetase) and product content (nitrate and nitrite) of nitrogen metabolism were also found to be increased, and this inhibited the accumulation of ammonium ions. After the seedlings were placed at 25°C for 24 h, the heterogeneous rootstocks could rapidly restore the growth of the bitter gourd seedlings by promoting the antioxidant and carbon and nitrogen metabolism systems. When luffa was used as rootstock, its performance on the indexes was better than that of pumpkin. The correlation between the various indicators was demonstrated using a principal component and correlation analysis.DiscussionThe luffa rootstock was found to be more conducive to reducing cell damage and energy loss in bitter gourd seedlings caused by heat induction through the maintenance of intracellular redox homeostasis and the promotion of carbon and nitrogen metabolism.

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Section: Discussionsupporting

confidence: 86%

Grafting promoted antioxidant capacity and carbon and nitrogen metabolism of bitter gourd seedlings under heat stress

Wen

Lian³

et al. 2022

Front. Plant Sci.

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“…As typical compatible osmolytes, they contribute to the preservation of water within plant cells, thereby reducing water availability for ice nucleation in the apoplast ( Markovskaya et al, 2010 ). In recent research, the sucrose phosphate synthase (SPS) activity decreased in the SPSA1 mutant of Arabidopsis , which resulted in an increase of starch synthesis ( Sun et al, 2011 ); the activities of acid invertase and SS in Cerasus pseudocerasus under cold stress were significantly increased ( Turhan and Ergin, 2012 ). Low temperature can induce the expression of TaSS and TaSPS and increase the soluble sugar content ( Zeng et al, 2011 ).…”

Section: Discussionmentioning

confidence: 99%

VvBAP1 Is Involved in Cold Tolerance in Vitis vinifera L.

et al. 2018

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The majority of commercial grape cultivars originate from the European grape. While these cultivars have excellent organoleptic qualities, they suffer from a relatively poor tolerance to the cold experienced during winter, resulting in significant damage to grapevines. Thus, low temperature is one of the bottlenecks that restrict the further growth of the grape industry. Research on the mechanism of cold tolerance in grapes is therefore very important. BON association protein 1 (BAP1) is a recently discovered phospholipid-binding protein. In Arabidopsis, the expression of AtBAP1 can be regulated via low temperature; however, the function of BAP1 in the grapevine has not been reported. The VvBAP1 gene was cloned in our previous studies in grapes, and bioinformatics analysis showed that it harbors the conservative calcium-dependent C2 protein domain. However, little is known about its function and underlying mechanism. In this study, cold treatment was applied to the cold-resistant grape varieties ‘F-242’ and ‘Zuoyouhong’ as well as to the cold-sensitive grape varieties ‘Cabernet Sauvignon’ and ‘Chardonnay.’ The expression level of VvBAP1 in the cold-resistant varieties was significantly higher than in the cold-sensitive varieties, indicating that VvBAP1 could be associated with the cold response processes in the grapevine. Using the cold-resistant grape variety ‘F-242’ as material, with the 4°C and CaCl2 treatment, the relative expression of VvBAP1 was determined via qRT-PCR. Both low temperature and low-temperature signal Ca2+ induced VvBAP1 expression. In addition, the VvBAP1 gene was cloned and transferred into Arabidopsis to generate VvBAP1 overexpressing plants. Biochemical assays and gene expression analyses were conducted on plants subjected to low temperature treatments (4 and -8°C). The obtained results showed that the activities of superoxide dismutase and peroxidase in these transgenic plants were higher than those in wild type (WT) plants, and that cell membrane permeability and malondialdehyde content were both lower compared to WT plants. Furthermore, the content of soluble sugars and the expression levels of sugar-metabolizing related genes, such as BAM4-7, SS4, and G6PD5, were significantly higher than those of WT plants. Furthermore, the expression of low temperature response signal genes, including CBF1, CBF3, COR15a, COR6.6, COR27, and KIN1, were also enhanced. In summary, these results showed that VvBAP1 could strengthen the cold resistance in the grapevine through adjusting and controlling the sugar content and activating antioxidant enzyme activity.

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“…Further analysis showed that CsINV5 might be a vacuolar INV that modulates the concentration and remobilization of Suc stored in the vacuole (data not shown). Recently, several studies suggested that invertase had a close relationship with the plant cold response (Turhan and Ergin 2012;Zhang et al 2011); however, its role in the cold response remains mysterious because its activities are controlled by post-translational modification or invertase inhibitors. The different expression patterns of the CsSUS genes might result from the bidirectional role of SUS in the conversion of Suc to Glc and Fru, which is necessary for Suc balance in the plant (Ruan 2014).…”

Section: Sugar Accumulated Through the Induction Of Sugar Metabolism mentioning

confidence: 99%

Effects of cold acclimation on sugar metabolism and sugar-related gene expression in tea plant during the winter season

et al. 2015

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Sugar plays an essential role in plant cold acclimation (CA), but the interaction between CA and sugar remains unclear in tea plants. In this study, during the whole winter season, we investigated the variations of sugar contents and the expression of a large number of sugar-related genes in tea leaves. Results indicated that cold tolerance of tea plant was improved with the development of CA during early winter season. At this stage, starch was dramatically degraded, whereas the content of total sugars and several specific sugars including sucrose, glucose and fructose were constantly elevated. Beyond the CA stage, the content of starch was maintained at a low level during winter hardiness (WH) period and then was elevated during de-acclimation (DC) period. Conversely, the content of sugar reached a peak at WH stage followed by a decrease during DC stage. Moreover, gene expression results showed that, during CA period, sugar metabolism-related genes exhibited different expression pattern, in which beta-amylase gene (CsBAM), invertase gene (CsINV5) and raffinose synthase gene (CsRS2) engaged in starch, sucrose and raffinose metabolism respectively were solidly up-regulated; the expressions of sugar transporters were stimulated in general except the down-regulations of CsSWEET2, 3, 16, CsERD6.7 and CsINT2; interestingly, the sugar-signaling related CsHXK3 and CsHXK2 had opposite expression patterns at the early stage of CA. These provided comprehensive insight into the effects of CA on carbohydrates indicating that sugar accumulation contributes to tea plant cold tolerance during winter season, and a simply model of sugar regulation in response to cold stimuli is proposed.

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Soluble Sugars and Sucrose-Metabolizing Enzymes Related to Cold Acclimation of Sweet Cherry Cultivars Grafted on Different Rootstocks

Cited by 25 publications

References 40 publications

Grafting promoted antioxidant capacity and carbon and nitrogen metabolism of bitter gourd seedlings under heat stress

Grafting promoted antioxidant capacity and carbon and nitrogen metabolism of bitter gourd seedlings under heat stress

VvBAP1 Is Involved in Cold Tolerance in Vitis vinifera L.

Effects of cold acclimation on sugar metabolism and sugar-related gene expression in tea plant during the winter season

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