Soluble Specific Leptospiral Complement-Fixing Antigens

Ezell, Sam B.; Hoag, Warren G.; Warner, Albert R.; Yager, Robert H.; Gochenour, William S.

doi:10.3181/00379727-80-19576

Cited by 9 publications

(6 citation statements)

References 2 publications

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“…Loss of complement fixation inhibitory effect of NDTM antigen by treatment with proteolytic enzymes. Treatment of NDTM antigen with Nagase or Pronase resulted in 4. Inhibition of complement fixation between TM antigen and immune serum by NDTM antigen and other fractions of TM antigen.…”

Section: Resultsmentioning

confidence: 99%

“…There have been reports assuming that the serotype specificity of leptospiral antigen was (4,5,7,8,(12)(13)(14)17), as in gram-negative bacteria. However, some findings have also been reported which might not support the role of carbohydrate in the serotype specificity of leptospiras.…”

Section: Resultsmentioning

confidence: 99%

“…There have been several reports (4,5,7,8,(11)(12)(13)(14)17) on the isolation and characterization of leptospiral antigens. A soluble, highly strainspecific complement-fixing antigen which was heat stable and non-protein was obtained from 10 serotypes by Ezell et al (4). The complement-fixing principles obtained from Leptospira icterohaemorrhagiae, L. canicola, and L. bataviae by Schneider (13) were in polysaccharide-containing cell fractions relatively free of protein.…”

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confidence: 99%

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Possible Role of Protein(s) as Antigenic Determinant of the Type-Specific Main Antigen of Leptospira kremastos Strain Kyoto

Adachi

Yanagawa

1975

Infect Immun

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Acid hydrolysates of the type-specific main (TM) antigen isolated from Leptospira kremastos strain Kyoto showed a considerable inhibition of complement fixation reaction between TM antigen and anti-strain Kyoto serum. A fraction of 2 N HCI hydrolysate containing exclusively a large amount of neutral sugars showed no inhibition, whereas another fraction containing only a small amount of protein(s) showed a slight inhibition. The remaining fractions containing amino sugars, protein(s), and neutral sugars showed a slight inhibition, but the inhibition did not correlate with the amount of amino sugars. TM antigen, delipidized by hydrolysis with 1 N HCI for 4 min and then dialysis, showed a strong inhibition. The lipid fraction did not show any inhibition. The inhibitory effect of nondialyzable delipidized TM antigen was completely lost by treating the antigen with proteolytic enzymes, indicating that the antigenic determinant of L. kremastos strain Kyoto is intimately related with protein(s).

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

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Possible Role of Protein(s) as Antigenic Determinant of the Type-Specific Main Antigen of Leptospira kremastos Strain Kyoto

Adachi

Yanagawa

1975

Infect Immun

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“…in 1933 was a heat-killed product and, as our work was commencing, Ezell et al (1952) reported that leptospiral culture ·liquor contained a heat· stable complement-fixing antigen. We therefore, decided to use heat-killed whole culture as Our vaccine.…”

Section: Preparation Of Vaccinementioning

confidence: 95%

Immunization againstLeptospira pomona

Webster

Reynolds

1955

New Zealand Veterinary Journal

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“…There have been several reports on the isolation and characterization of leptospiral antigens (Hindle & White, 1933;Ezell et al, 1952;Schneider, 1954;Rothstein & Hiatt, 1956;Schricker & Hanson, 1963;Shinagawa & Yanagawa, 1972;Faine et al, 1974;Adler et al, 1980;Palit & Harrison, 1980). The identification of antigenic determinant groups of leptospira forms the basis for the classification of leptospiras.…”

Section: Introductionmentioning

confidence: 96%

Isolation of an Antigenic Oligosaccharide Fraction from Leptospira interrogans serovar canicola with a Monoclonal Antibody

1984

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An oligosaccharide fraction containing the antigenic determinant of lipopolysaccharide antigen (TM antigen) from Leptospira interrogans serovar canicola, recognized by a monoclonal antibody (CT3) which agglutinates serovars canicola and broomi, was isolated by formic acid and successive sulphuric acid hydrolyses. Separation of the antigenic compounds was done by Bio-Gel P-2 and Sephadex G-25 gel filtration, and high-performance liquid chromatography with two different columns. The fraction finally obtained was a mixture of two oligosaccharides, both of which migrated as a single spot having a slightly higher mobility than an authentic tetrasaccharide (stachyose) on thin layer chromatography. The fraction contained rhamnose, arabinose and two major and two minor unknown sugars which were shown to be N- or O-acetylated and/or O-methylated sugars by nuclear magnetic resonance. The fraction inhibited the binding of CT3 antibody with TM antigen in enzyme-linked immunosorbent assay and microscopic agglutination of serovar canicola with the antibody. The inhibitory activity was destroyed by periodate oxidation or mild alkaline treatment, but was resistant to sodium borohydride reduction.

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Soluble Specific Leptospiral Complement-Fixing Antigens

Cited by 9 publications

References 2 publications

Possible Role of Protein(s) as Antigenic Determinant of the Type-Specific Main Antigen of Leptospira kremastos Strain Kyoto

Possible Role of Protein(s) as Antigenic Determinant of the Type-Specific Main Antigen of Leptospira kremastos Strain Kyoto

Immunization againstLeptospira pomona

Isolation of an Antigenic Oligosaccharide Fraction from Leptospira interrogans serovar canicola with a Monoclonal Antibody

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