Soluble Signalling Factors Derived from Differentiated Cartilage Tissue Affect Chondrogenic Differentiation of Rat Adult Marrow Stromal Cells

Ahmed, Nazish; Dreier, Rita; Göpferich, Achim; Grifka, Joachim; Grässel, Susanne

doi:10.1159/000107728

Cited by 83 publications

(93 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In recent years, a number of studies have demonstrated chondrocyte-mediated chondrogenic differentiation of stem cells [36,[41][42][43]. A variety of unidentified morphogenetic factors from chondrocytes such as growth factors, hormones, and ECM influence the chondrogenesis of LVEC cells either individually or in synergy with other factors such as TGF-b1.…”

Section: Discussionmentioning

confidence: 99%

Engineering Musculoskeletal Tissues with Human Embryonic Germ Cell Derivatives

et al. 2010

View full text Add to dashboard Cite

The cells derived from differentiating embryoid bodies of human embryonic germ (hEG) cells express a broad spectrum of gene markers and have been induced toward ectoand endodermal lineages. We describe here in vitro and in vivo differentiation of hEG-derived cells (LVEC line) toward mesenchymal tissues. The LVEC cells express many surface marker proteins characteristic of mesenchymal stem cells and differentiated into cartilage, bone, and fat. Homogenous hyaline cartilage was generated from cells after 63 population doublings. In vivo results demonstrate cell survival, differentiation, and tissue formation. The high proliferative capacity of hEG-derived cells and their ability to differentiate and form three-dimensional mesenchymal tissues without teratoma formation underscores their significant potential for regenerative medicine. The adopted coculture system also provides new insights into how a microenvironment comprised of extracellular and cellular components may be harnessed to generate hierarchically complex tissues from pluripotent cells. STEM CELLS 2010;28:765-774 Disclosure of potential conflicts of interest is found at the end of this article.

show abstract

Section: Discussionmentioning

confidence: 99%

Engineering Musculoskeletal Tissues with Human Embryonic Germ Cell Derivatives

et al. 2010

View full text Add to dashboard Cite

show abstract

“…For example, it was reported that cartilage explants secreted TGF-b for up to 14 days, and soluble factors derived from cartilage explants were able to upregulate SOX9 gene expression and type II collagen synthesis in MSCs. 14 Similar studies confirmed that soluble factors derived from chondrocytes were able to upregulate chondrogenic gene markers and matrix production by MSCs, 15,16 and reduce hypertrophic markers in MCSs. 14,17 However, the chondrogenesis induction reported via chondrocyte-conditioned media showed only moderate type II collagen gene upgregulation over control media and predominately type I collagen matrix accumulation.…”

mentioning

confidence: 57%

“…While coculture of these two populations has been reported, [14][15][16][17][18][19] results have been mixed, and there are no reports of zonal coculture models. A clear trend has not been established on the influence or the mechanisms of influence between these two cell populations.…”

mentioning

confidence: 99%

Engineering Superficial Zone Chondrocytes from Mesenchymal Stem Cells

Coates

Fisher

2014

Tissue Engineering Part C: Methods

View full text Add to dashboard Cite

Recent cartilage engineering efforts have focused on development of zonally organized tissue. However, there remains a need for protocols that differentiate progenitor populations into chondrocytes of zonal phenotype. Here, we evaluate the potential of coculture of bovine mesenchymal stem cells (MSCs) and zonal explants of bovine cartilage tissue to drive MSC differentiation to chondrocytes with the superficial zone phenotype. Two coculture systems were set up: one between alginate encapsulated MSCs and superficial zone cartilage explants, and one between MSCs and middle/deep zone cartilage explants. Chondrogenic and superficial zone markers were monitored over a 21-day differentiation period via gene and protein expression. A control conditioned media study was used to determine the impact of communication via soluble factors between cell populations during differentiation. At day 21, results show superficial zone explant coculture without transforming-growth factor b3 supplementation induces upregulation of chondrogenic gene expression markers SOX9 and type II collagen 3.4-fold and 11.4-fold, respectively, over standard chondrogenic control media. Further, coculture of MSCs and superficial zone explants can be used to upregulate mRNA expression of the superficial zone marker proteoglycan-4 in MSCs (1.75-fold over chondrogenic control at day 21), indicating the superficial zone chondrocyte phenotype. Gene expression data show middle/deep zone explant and MSC coculture did not induce the chondrogenesis observed in superficial zone explant coculture. Likewise, poor chondrogenesis was observed in all conditioned media groups. Results highlight the importance of superficial zone cartilage and cells in guiding stem cell fate and regulating differentiation of MSCs to chondrocytes of the superficial zone type.

show abstract

“…35 Then, the cells were recovered following 10 min of centrifugation at 260 rcf and washed with D-PBS. A minimum of 1 · 10 5 cells were thus prepared, lysed in TRIzol reagent (Invitrogen), and stored at -80°C.…”

Section: Reverse Transcription-polymerase Chain Reaction Analysis Of mentioning

confidence: 99%