Soluble receptor for advanced glycation end products in late-onset neonatal infection

Abstract

OBJECTIVES: To assess the plasma levels of sRAGE (soluble receptor for advanced glycation end products) in infected and non-infected preterm neonates and to compare their diagnostic values with standard infection biomarkers. BACKGROUND: RAGE activates pathways responsible for acute and chronic infl ammation. The soluble type of this receptor, sRAGE, which acts as a decoy receptor, has been linked to the severity of sepsis and its outcome. METHODS: Prospective clinical study was carried out from January 2011 to August 2013. There were 33 neonates included according to their infection status and divided into subgroups as follows: infected (I), septic (S), non-infected controls (C). RESULTS: We found signifi cantly lower values of sRAGE in the subgroup S (905.54±220.53 pg/mL; p < 0.028), while borderline values were higher in the subgroup I vs C (2158.33±197.33 pg/mL vs 1744.80±157.74 pg/mL; p < 0.064). By analysing the interobserver concordance we detected 70 % agreement as to sRAGE values detected in neonatal late-onset infections and sepsis, while procalcitonin was used as golden standard. CONCLUSION: Plasma sRAGE values refl ect the severity of the infl ammatory status in late-onset infection and sepsis in preterm neonates. Our results indicate that sRAGE could be a good potential biomarker of late-onset neonatal infection and sepsis (Tab. 2, Ref. 14). Text in PDF www.elis.sk. KEY WORDS: sRAGE, biomarker, preterm neonate, late-onset infection, late-onset sepsis. Acknowledgement: This work was supported by project "Support of human resources development using the most modern methods and forms of education on JFM CU in Martin" co-fi nanced by EU sources and European Social Fund. We thank all the research staff of Department of Neonatology JFM CU and UH Martin who worked on recruitment and sample collection, as well as staff at the Department of Clinical Biochemistry UHM, and participating families. This work was supported by project "Support of human resources development using the most modern methods and forms of education on JFM CU in Martin" co-fi nanced by EU sources and European Social Fund.

Diagnostic Potential of Evaluation of SDF-1αand sRAGE Levels in Threatened Premature Labor

“…This finding strongly suggests that reduced sRAGE levels enhance the inflammatory response in women with premature labor. Research from other branches of medicine has suggested that low sRAGE concentrations augment inflammation, indicating that sRAGE is an independent risk factor of approaching intensification of the disease [ 39 , 53 – 55 ]. We previously found that decreased sRAGE levels were an independent predictive parameter of preterm delivery in women with threatened premature labor with intact membranes [ 56 , 57 ].…”

The health of children born to mothers who have had a threat of abortion

“…В настоящее время в открытых базах данных имеется небольшое число сообщений об особенностях содержания в крови RAGE и его лигандов при различных осложнениях гестации и патологии новорожденных [20][21][22][23][24][25]. В работе M. Constantine и соавт.…”

“…(2017) [17] сообщили о снижении концентрации sRAGE в пуповинной крови у детей с тяжелым поражением ЦНС, родившихся в сроке до 34 нед гестации. Кроме того, имеются данные о повышении уровня одного из лигандов RAGE белка -HMGB1 у женщин с хориоамнионитом и преждевременными родами [25] и снижении содержания sRAGE в крови у новорожденных с сепсисом [22].…”

DNA-based nanowires and nanodevices

“…The conducting current through a Ni-DNA nanowire may be altered by the interaction with proteins, DNA, RNA and small compounds [50,51] or by modifications, such as methylation and damages [52]. Hence, biosensing systems can be developed by integrating Ni-DNA devices for the detection of substances that can interact with DNA, transcription factors or regulators of genes and molecules that cause DNA modification and damages.…”