Soluble Proteins, Proline, Carbohydrates and Na+/K+ Changes in Two Tomato (Lycopersicon esculentum Mill.) Cultivars under in vitro Salt Stress

Amini, Fariba; Ehsanpour, Ali Akbar

doi:10.3844/ajbbsp.2005.212.216

Cited by 85 publications

(71 citation statements)

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“…In vitro culture is a valid and rapid tool for studying the behavior of plants in response to salt stress, while other factors (nutrients, lighting, temperature) are held constant and controlled in an optimal manner. This approach has been successfully used for the evaluation of salt stress in brassica [8], tomato [9][10][11][12][13][14][15][16], mulberry [17,18], potato [19], rice [20][21][22], poplar [23,24], strawberry [25], eucalyptus [26][27][28], grapevine [29,30], citrus [31,32], and pistacia [33].…”

Section: Introductionmentioning

confidence: 99%

Alfalfa (Medicago sativa L.) clones tolerant to salt stress: in vitro selection

et al. 2013

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In order to quickly and efficiently evaluate the salt tolerance of alfalfa, salinity tests were conducted on Medicago sativa L. var. australis, var. icon, var. loi, and var. gea, under in vitro conditions. Pregerminated seeds of four varieties were subjected to five different NaCl concentrations (0, 50, 100, 150, 200 mM). The influence of saline stress was estimated on the basis of survival percentage, growth parameters, and electrolyte leakage. The seedlings surviving on the medium enriched with salt at the highest concentration were presumed to be tolerant and represented the mother plants for the production of in vitro clones. In the following step, the clones were evaluated in vitro to confirm the salt tolerance. The influence of mild salt stress (75 mM NaCl) on the growth parameters of selected clones was examined. At the end of this trial, the proline accumulation and sodium content in alfalfa shoots were also quantified. The results suggest an increased level of proline promotes salt tolerance. Medicago sativa L. var. icon is highly tolerant in comparison with the other varieties tested. In vitro selection of M. sativa L. varieties on salt-containing media allowed us to obtain clones with increased salinity tolerance.

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Section: Introductionmentioning

confidence: 99%

Alfalfa (Medicago sativa L.) clones tolerant to salt stress: in vitro selection

et al. 2013

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“…In vitro techniques make it possible to screen the required number of genotypes rapidly since in vitro plant cultures, even at different stages of development, may exhibit their capacity to withstand the stress (Tewary et al 2000;Zaki et al 2016). Several researchers have proposed using in vitro screening to identify tomato germplasm with salinity tolerance (Amini and Ehsanpour 2005;Cano et al 1996;Cano et al 1995;Cuartero et al 2006;Cuartero et al 1992;Emilio et al 1998;Garcia-Reina 1988). Fewer of these studies have examined the salinity tolerance in cultivated tomatoes and their wild species.…”

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confidence: 99%

“…Salinity stress affects plants through morphological, physiological and metabolic changes in their organs (Amini and Ehsanpour 2005;Zhang et al 2004). Perhaps the best investigated of the traits related to salt tolerance are those associated with the ion content of plants grown in the presence of salt stress (Abel 1969;Tal and Shannon, 1983).…”

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confidence: 99%

A comparative <i>in vitro</i> study of salt tolerance in cultivated tomato and related wild species

Zaki

Yokoi

2016

Plant Biotechnology

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Salinity stress is a major abiotic stress for plants worldwide. This study was carried out to determine the variation in salt tolerance for 12 different genotypes belonging to three different tomato species: Solanum lycopersicum (L), S. peruvianum (L) and S. pimpinellifolium (L). Shoot apices and callus cultures were exposed to different levels of salinity stress ranging from no salt (control) to 100, 200 and 300 mmol L −1 NaCl. All growth and physiological parameters were significantly affected by salt stress. Most shoot apices of S. lycopersicum did not develop roots when exposed to low NaCl levels, whereas apices of S. peruvianum and S. pimpinellifolium developed roots when exposed to all salt levels. This difference in salt tolerance was clearly shown on the basis of root fresh weights and root surface areas. Callus growth in response to increased salinity was much greater in S. peruvianum and S. pimpinellifolium than in S. lycopersicum. The Cl − and Na + concentrations increased significantly with increasing salt in the three species, although the S. peruvianum lines accumulated more ions compared with the others. As the salt concentration increased, less K + accumulated in S. lycopersicum compared to the related wild species. The results obtained in this study suggest that S. peruvianum line 0043-1 was the accession with the best salt tolerance. The most tolerant cultivated tomato (S. lycopersicum) cultivar was 'Rutgers. ' Both S. peruvianum line 0043-1 and S. lycopersicum 'Rutgers' are good candidates for inclusion in tomato breeding programs for salt-tolerance.

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“…The changes of soluble proteins content in plants under stress conditions are responsible for adaption in metabolic pathways [64]. Protein synthesis and degradation respond differently to stress conditions according to the stress resistance of plants.…”

Section: Discussionmentioning

confidence: 99%

The Biomass and Physiological Responses of Vallisneria natans (Lour.) Hara to Epiphytic Algae and Different Nitrate-N Concentrations in the Water Column

Min

Zuo

Zhang

et al. 2017

Water

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Abstract:Increasing N concentration and the high density of epiphytic algae are both key factors leading to the decline of submerged macrophytes in many eutrophic lakes. In order to investigate the impacts of increased nitrate-N concentration and the growth of epiphytic algae on the decline of submerged vegetation, we conducted a 2 × 4 factorial experiment with the submerged macrophyte Vallisneria natans (Lour.) Hara by measuring the biomass of plants and some physiological indexes in leaves of V. natans under four nitrate-N concentrations in the water column (0.5, 2.5, 5, and 10 mg/L) and two epiphytic groups (epiphytic algae group and no epiphytic algae group). The results suggested that epiphytic algae could impose adverse effects on the biomass accumulation of V. natans, while the increasing nitrate-N concentration (0.5-10 mg/L) could oppositely promote this process and counteract the adverse effect of epiphytic algae. When nitrate-N concentration was 5 mg/L, the total chlorophyll content in leaves of V. natans in the epiphytic algae group was prominently lower compared with the no epiphytic algae group, while MDA, free proline, and anti-oxidant enzyme (SOD, POD, CAT) activities were significantly higher. Overhigh nitrate-N concentration in the water column also directly imposed adverse effects on the physiology of V. natans. When nitrate-N concentration was over 5 mg/L, the total chlorophyll content and free proline decreased in the no epiphytic algae group, while soluble carbohydrates and soluble proteins decreased when nitrate-N was over 2.5 mg/L. Meanwhile, epiphytic algae and nitrate-N content imposed a synergetic effect on the anti-oxidant enzyme activities of V. natans. When nitrate-N concentration was over 5 mg/L, SOD, POD, and CAT activities kept constant or decreased, which indicated that the oxidation resistance of V. natans was inhibited by stress. Our results indicate that epiphytic algae and increasing nitrate-N concentration in the water column could severally or synergistically impose adverse effects on the physiology of submerged macrophytes and are both key factors leading to the decline of submerged macrophytes.

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Soluble Proteins, Proline, Carbohydrates and Na+/K+ Changes in Two Tomato (Lycopersicon esculentum Mill.) Cultivars under in vitro Salt Stress

Cited by 85 publications

References 0 publications

Alfalfa (Medicago sativa L.) clones tolerant to salt stress: in vitro selection

Alfalfa (Medicago sativa L.) clones tolerant to salt stress: in vitro selection

A comparative <i>in vitro</i> study of salt tolerance in cultivated tomato and related wild species

The Biomass and Physiological Responses of Vallisneria natans (Lour.) Hara to Epiphytic Algae and Different Nitrate-N Concentrations in the Water Column

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