Soluble proteins and isoesterases as taxonomic markers tested on nine wildSolanum species and eight Slovakian potato varieties

Heldák, Ján; Ŝubová, D.

doi:10.1007/bf02358180

Cited by 3 publications

(5 citation statements)

References 8 publications

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“…On the contrary, Kormuák et al (1999) found 1014 bands in soluble protein profiles of eight Slovakian potato cultivars analysed under similar conditions. The observed bands cannot be evaluated as equivalent because they have different thickness and staining intensities.…”

Section: Resultsmentioning

confidence: 88%

“…Whereas Kormuák et al (1999) observed a relatively low number of isoesterases in a set of eight Slovakian potato cultivars, Douches and Lundlam (1991) evaluated the banding pattern created by EST-C locus in a set of 116 North American cultivars as very complicat- ed and difficult to interpret. In agreement with this opinion, Burton (1989) reported that esterases separated in polyacrylamide gel (pH 8.9) had a richer pattern.…”

Section: Resultsmentioning

confidence: 99%

“…The bands of esterase activity are in a region with Rm from 0.403 up to 0.613 ( Figure 5). Giovannini et al (1993) and Kormuák et al (1999) reported the region occurrence for isoesterases on polyacrylamide gel in the range of Rm 0.6000.900, however these authors used only 6% gel. Coefficients of similarity between the analysed cultivars were observed in the range from 0.540 up to 1.000 (Figure 6).…”

Section: Resultsmentioning

confidence: 99%

“…The profiles of tuber soluble proteins, esterases, and peroxidases were applied in the studies of relationship and plant genetics of cultivated and wild potato species and their hybrids (Desborough and Peloquin 1965, 1966, 1968, Simon and Peloquin 1980, Giovannini et al 1993, Kormuák et al 1999.…”

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confidence: 99%

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Study of biochemical variability of potato cultivars by soluble protein, isoesterase, and isoperoxidase electrophoretic patterns

Bárta¹,

Čurn²,

Diviš³

2003

PLANT SOIL ENVIRON

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Biochemical variability between thirteen European and five Czech potato (Solanum tuberosum L.) cultivars grown in the Czech Republic was studied by soluble protein, isoesterase, and isoperoxidase electrophoretic patterns. It was confirmed that cultivar differences in protein polymorphism can be revealed by applied electrophoretic patterns. It was shown that the different character of protein and isozyme profiles required different approaches to their evaluation. For complex patterns such as electrophoretic soluble protein spectra, it is more convenient to use the evaluation of their absorbance profiles and for simpler profiles of isozymes the evaluation based on the presence or absence of a band in a definite position (simple matching) should be used. In spite of the complexity of tetraploid disposition of analysed cultivars, the results suggested higher similarity of profiles between relative cultivars and they also indicated the existence of higher similarity between cultivars from the same breeding firm.

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Section: Resultsmentioning

confidence: 88%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Study of biochemical variability of potato cultivars by soluble protein, isoesterase, and isoperoxidase electrophoretic patterns

Bárta¹,

Čurn²,

Diviš³

2003

PLANT SOIL ENVIRON

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“…Molecular markers are used in genetic studies (Kardolus et al 1998), variety differentiation (Kormuťák et al 1999;Braun & Wenzel 2004/5;Braun et al 2004/5;Moisan-Thiery et al 2005), identification of genes conferring resistance to diseases and pests (Niewöhner et al 1995;Celebi-Toprak et al 2002) and in marker-assisted selection (MAS). MAS in the breeding context involves scoring indirectly for the presence or absence of a desired phenotype based on banding patterns of molecular markers located in or near the genes controlling resistance to PVS (Marczewski et al 2001(Marczewski et al , 2002, resistance to PVX (Ritter et al 1991;Kanyuka et al 1999), resistance to PVM (Marczewski et al 2006) and resistance to PVY.…”

Section: Discussionmentioning

confidence: 99%

Selection of DNA markers for detection of extreme resistance to potato virus Y in tetraploid potato (Solanum tuberosum L.) F<sub>1</sub> progenies

Heldák¹,

Bežo²,

Štefúnová³

et al. 2007

CZECH J GENET PLANT

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Abstract:Marker-assisted selection is progressively introduced into potato breeding especially in the case of monogenically inherited traits. Varieties and clones with extreme resistance to potato virus Y (PVY) from German, Hungarian, Polish, Dutch and Slovak breeding programmes were used as female parents in 6 crosses. F 1 progenies were subjected to the bulk segregant analysis. Polymerase chain reaction (PCR) based markers for Ry sto and Ry adg viral resistance alleles were used for the evaluation of varieties, clones and seedlings of F 1 progenies. All resistant F 1 genotypes were reliably identified using three molecular markers, STM0003, GP122 718 and GP122 406 , associated with the Ry sto allele. No genotype corresponded with phenotypic data and Ry adg markers. Ry adg and three selected Ry sto markers were applied to 40 genotypes from an in vitro gene bank and 14 genotypes were recognized to have Ry sto and none of them possessed Ry adg . Indirect selection is based on DNA polymorphism linked to the extreme resistance to PVY resulting in error when polymorphism is not conserved in all genetic backgrounds. A failure to detect extreme resistance to PVY using the Ry sto markers in variety Santé, resistant progeny of variety Santé and clone Y01-30 may be linked to recombination events or different Solanum stoloniferum background.

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Characterisation of Potato (Solanum tuberosum L.) Varieties by Electrophoresis of Tuber Proteins

Sýkorová¹,

Matějová²

2006

CZECH J GENET PLANT

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Abstract:The modified PAGE method (TRIS -Glycine buffer pH 8.9) was used for the characterisation of selected 25 registered potato varieties. This method enabled to identify each variety from the examined variety set. The calculation of identity indexes (proportion of common bands) helped to evaluate the similarity of varieties from these aspects. The examination of electrophoretic profiles of soluble tuber proteins, which are highly polymorphic and stable, can be considered as valuable for variety characterisation and identification.

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Soluble proteins and isoesterases as taxonomic markers tested on nine wildSolanum species and eight Slovakian potato varieties

Cited by 3 publications

References 8 publications

Study of biochemical variability of potato cultivars by soluble protein, isoesterase, and isoperoxidase electrophoretic patterns

Study of biochemical variability of potato cultivars by soluble protein, isoesterase, and isoperoxidase electrophoretic patterns

Selection of DNA markers for detection of extreme resistance to potato virus Y in tetraploid potato (Solanum tuberosum L.) F<sub>1</sub> progenies

Characterisation of Potato (Solanum tuberosum L.) Varieties by Electrophoresis of Tuber Proteins

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