Soluble p‐selectin should be measured in citrated plasma, not in serum

Measurement of soluble P‐selectin and soluble CD40 ligand in serum and plasma

“…It is generally assumed that research on the genetics of complex diseases requires representative, often large, study sample materials [1,2]. In large population studies, DNA collection has already largely become a routine procedure [3][4][5]. The DNA yield from whole blood samples varies between different storage conditions and extraction methods but also between individuals approaching normality.…”

“…However, platelets are activated during the process of blood clotting. Therefore serum compared with plasma may contain significantly higher levels of soluble platelet activation markers [4][5][6].…”

Baseline level of platelet-leukocyte aggregates, platelet CD63 expression, and soluble P-selectin concentration in patientswith posttraumatic stress disorder: A pilot study

“…We should note that serum values differ from plasma values of (s)P-selectin (Caine and Blann, 2003;Thom et al, 2004) and future studies should address citrated plasma concentrations to confirm our results.…”

Increased serum levels of interleukin-6 and von Willenbrand Factor in early phase of acute coronary syndrome in a young and multiethnic Malaysian population

“…It was reported that the difference in P-selectin level between diseased and healthy patients was significantly found only in the plasma sample, as the serum samples may not truly reflect the in vivo platelet activation activity of P-selectin 26. The detection of P-selectin level in serum samples may be due to excess P-selectin released from activated platelets or by the process of coagulation and clot formation 26. Nonetheless, it was reported that although P-selectin levels are significantly higher in serum compared with plasma, the absolute concentration of P-selectin remain significantly and moderately correlated in both serum and plasma.…”