Soluble P-selectin and correlation with Prothrombin Fragment 1 + 2 in myeloid malignancies in Cipto Mangunkusumo general hospital

Abstract

Background Myeloid cells express microparticles that could increase the expression of adhesion molecules including P-selectin. We aimed to evaluate the level of soluble P-selectin (sP-selectin) and prothrombin fragment 1 + 2 (F1 + 2), and to determine correlation of sP-selectin with leukocyte count and F1 + 2 levels in acute myeloid leukemia (AML) and chronic myeloid leukemia (CML) patients. Methods Patients with newly diagnosed AML (n = 25), CML (n = 13), and controls (n = 17) were recruited in this study. The diagnosis of AML and CML is based on 2001 WHO and/or FAB criteria. Levels of sP-selectin and F1 + 2 were determined using enzyme-linked immunosorbent assay kits (Behring ELISA Processor-III® and Behring Enzygnost F1 + 2). Results sP-selectin was significantly elevated in CML patients compared to AML patients (p = 0.001). Levels of F1 + 2 in AML and CML patients were significantly increased in comparison to controls (p < 0.001 and p = 0.043). Levels of sP-selectin were significantly correlated to leukocyte count (r = 0.437; p = 0.029) and F1 + 2 (r = 0.436; p = 0.029) in AML patients. Conclusions AML and CML patients had an increased tendency to thrombosis. While CML patients had higher platelet and/or endothelial activation, hypercoagulable state are more pronounced in AML patients.

Elevated platelet–leukocyte complexes are associated with, but dispensable for myocardial ischemia–reperfusion injury

“…Functionally, however, integrin-dependent leukocyte rolling and adhesion were not impaired in KOWT mice lacking platelet-derived P-selectin. Having said this, soluble P-selectin, which is mainly shed from activated endothelium, can still activate leukocytes via PSGL-1 [ 37 , 52 , 58 ] without requiring platelet-derived P-selectin and PLC formation. In line, soluble P-selectin levels were comparable in KOWT and WTWT chimeras in our study, confirming that the endothelium is the major source of soluble P-selectin even post-myocardial infarction.…”