Soluble inflammation markers in nasal lavage from CF patients and healthy controls

Beiersdorf, N.; Schien, M.; Hentschel, Julia; Pfister, W.; Markert, Udo R.; Mainz, Jochen G.

doi:10.1016/j.jcf.2012.08.015

Cited by 23 publications

(38 citation statements)

References 47 publications

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“…Analysis of MMP9, MPO, RANTES, IL-1β, IL-6, IL-8 and TNF was carried out using a Cytometric Bead Array (FlowCytomix, eBioscience, San Diego, CA, USA) followed by flow cytometry (FACS Calibur, BD, Franklin Lakes, NJ, USA) as described elsewhere [14]. The results were evaluated using FlowCytomix Pro 2.3 software (eBioscience, Frankfurt, Germany).…”

Section: Methodsmentioning

confidence: 99%

“…Elevated MPO is an established marker for neutrophil activity as it is released in oxidative bursts. In the recent work by Beiersdorf et al [14], MPO was elevated in the NL of CF patients compared with healthy controls. Matrix metalloproteinase 9 (MMP9), also produced by neutrophils, is involved in the breakdown of extracellular matrix proteins such as elastin or collagen [22].…”

Section: Introductionmentioning

confidence: 95%

“…Systemic IL-6 levels correlated significantly to several clinical parameters in both stages of disease. In our previous study assessing the NL of CF patients [14], IL-1β and IL-6 were detected more frequently in CF compared with healthy controls. In contrast, TNF that had been elevated in the BAL of CF patients was not detectable in the NL-fluid (NLF) of CF and healthy controls [14].…”

Section: Introductionmentioning

confidence: 99%

“…In our previous study assessing the NL of CF patients [14], IL-1β and IL-6 were detected more frequently in CF compared with healthy controls. In contrast, TNF that had been elevated in the BAL of CF patients was not detectable in the NL-fluid (NLF) of CF and healthy controls [14]. However, especially for IL-1β, IL-6 and TNF, differences in cytokine expression between upper and lower airways and peripheral blood were observed, suggesting a compartmentalised local inflammatory response [15,16].…”

Section: Introductionmentioning

confidence: 99%

“…IL-8 encourages neutrophils to leave the circulation and migrate into the tissue. In the NLF of CF patients, IL-8 was detected more frequently than in healthy controls [14]. An increase of PMNs and IL-8 in the upper airways of CF patients has also been reported [17].…”

Section: Introductionmentioning

confidence: 99%

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Dynamics of soluble and cellular inflammatory markers in nasal lavage obtained from Cystic Fibrosis patients during intravenous antibiotic treatment

et al. 2014

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BackgroundIn cystic fibrosis (CF) patients, the upper airways display the same ion channel defect as evident in the lungs, resulting in chronic inflammation and infection. Recognition of the sinonasal area as a site of first and persistent infection with pathogens, such as Pseudomonas aeruginosa, reinforces the “one-airway” hypothesis. Therefore, we assessed the effect of systemic antibiotics against pulmonary pathogens on sinonasal inflammation.MethodsNasal lavage fluid (NLF) from 17 CF patients was longitudinally collected prior to and during elective intravenous (i.v.) antibiotic treatment to reduce pathogen burden and resulting inflammation (median treatment time at time of analysis: 6 days). Samples were assessed microbiologically and cytologically. Cytokine and chemokine expression was measured by Cytometric Bead Array and ELISA (interleukin (IL)-1β, IL-6, IL-8, MPO, MMP9, RANTES and NE). Findings were compared with inflammatory markers from NLF obtained from 52 healthy controls.ResultsInitially, the total cell count of the NLF was significantly higher in CF patients than in controls. However after i.v. antibiotic treatment it decreased to a normal level. Compared with controls, detection frequencies and absolute concentrations of MPO, IL-8, IL-6 and IL-1β were also significantly higher in CF patients. The detection frequency of TNF was also higher. Furthermore, during i.v. therapy sinonasal concentrations of IL-6 decreased significantly (P = 0.0059), while RANTES and MMP9 levels decreased 10-fold and two-fold, respectively. PMN-Elastase, assessed for the first time in NFL, did not change during therapy.ConclusionsAnalysis of NLF inflammatory markers revealed considerable differences between controls and CF patients, with significant changes during systemic i.v. AB treatment within just 6 days. Thus, our data support further investigation into the collection of samples from the epithelial surface of the upper airways by nasal lavage as a potential diagnostic and research tool.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%