Soluble Fas in malignant pleural effusion and its expression in lung cancer cells

Mitani, Kayo; Nishioka, Yasuhiko; Yamabe, Kazue; Ogawa, Hirohisa; Miki, Toyokazu; Yanagawa, Hiroaki; Sone, Saburo

doi:10.1111/j.1349-7006.2003.tb01437.x

Cited by 23 publications

(17 citation statements)

References 34 publications

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“…Similar results have been shown in human hepatocellular carcinoma and human lung cancer cell lines [21,22]. The concentrations of soluble Fas/APO-1 antigen in HeLa cell line culture with all-trans-retinoic acid (ATRA) were downregulated when compared with HeLa cell line without ATRA.…”

Section: Discussionsupporting

confidence: 77%

Apoptosis of HeLa and CaSki cell lines incubated with All-trans retinoid acid.

Darmochwał-Kolarz

Gasowska-Giszczak²,

Paduch³

et al. 2010

Folia Histochem Cytobiol.

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Section: Discussionsupporting

confidence: 77%

Apoptosis of HeLa and CaSki cell lines incubated with All-trans retinoid acid.

Darmochwał-Kolarz

Gasowska-Giszczak²,

Paduch³

et al. 2010

Folia Histochem Cytobiol.

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“…After incubation for the indicated periods, all cells were collected, washed with PBS (-), and lysed with 1 ml of isogen (Nippon Gene). Total RNA was then extracted as described previously (25). HUVECs were used as a positive control.…”

Section: Rt-pcr Analysismentioning

confidence: 99%

“…The human lung adenocarcinoma PC-14 was a generous gift from Dr. N. Saijo (National Cancer Institute, Tokyo, Japan) (25). The cell line was maintained in RPMI 1640 medium supplemented with 10% FBS and penicillin/streptomycin (designated as CRPMI-1640) in a humidified incubator with a 5% CO 2 atmosphere at 37°C.…”

Section: Animal Experimentsmentioning

confidence: 99%

Expression of Soluble Vascular Endothelial Growth Factor Receptor-1 in Human Monocyte-Derived Mature Dendritic Cells Contributes to Their Antiangiogenic Property

Kishuku

Nishioka

Abe

et al. 2009

The Journal of Immunology

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The soluble form of vascular endothelial growth factor receptor-1 (sVEGFR-1) is produced from endothelial cells by alternative splicing of VEGFR-1 mRNA, and can inhibit angiogenesis by blocking the biological effects of VEGF. In this study, we show the expression of a large amount of sVEGFR-1 in human monocyte-derived mature dendritic cells (mDCs). As compared with monocytes and immature DCs, mDCs generated by TNF-α or soluble CD40L with IFN-γ, but not LPS or other stimuli, preferentially produce sVEGFR-1. We also detected the mRNA of sVEGFR-1 generated by alternative splicing of VEGFR-1 mRNA in mDCs induced by TNF-α. The production of sVEGFR-1 showed a distinct contrast to those of VEGF in each DC matured with various stimuli. The supernatant of DCs matured with TNF-α or soluble CD40L with IFN-γ showed inhibition of the tube formation of HUVECs, which was neutralized by anti-VEGFR-1 Ab, indicating that sVEGFR-1 secreted from mDCs was biologically active. Interestingly, the supernatant of mDCs generated with LPS increased HUVEC capillary-like formation in vitro. The ratio of sVEGFR-1 to VEGF clearly reflected the net angiogenic property of mDCs. Administration of mDCs induced by TNF-α into the s.c. tumor of PC-14 cells implanted in SCID mice demonstrated the inhibition of tumor growth via reduction of the number of CD31-positive vessels, indicating their in vivo antiangiogenic potential. These results suggest that sVEGFR-1 produced by mDCs contribute to their antiangiogenic property, and the ratio of sVEGFR-1 to VEGF might be a useful tool for evaluating their ability to regulate angiogenesis mediated by VEGF.

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“…Similarly, the soluble Fas protein, which is able to inhibit the immunogenic cells that will kill the tumor cells in its tumoral area, exists at a higher level in the malignant pleural effusions resulting from the lung cancer than the pleural effusions that emerged due to a reason other than cancer. Moreover, it was found that the soluble Fas was expressed by many lung cancer cell lines, such as H69, N291, RERF-LC-OK (Kagi et al, 1994;Mitani et al, 2003). It was determined that expression of the vascular endothelial growth factor (VEGF) was 10 times higher in the effusions of the patients with ovary, breast, and gastrointestinal carcinoma than that of the paired serum (Kraft et al,1999).…”

mentioning

confidence: 99%

Inhibitory Effects of Dunning Rat Prostate Tumor Fluid on Proliferation of the Metastatic MAT-LyLu Cell Line

Bugan

Altun²

2015

Asian Pacific Journal of Cancer Prevention

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Soluble Fas in malignant pleural effusion and its expression in lung cancer cells

Abstract: as (APO-1, CD95) is a 48-kDa type I transmembrane protein which belongs to the TNF (tumor necrosis factor) receptor superfamily.

Cited by 23 publications

References 34 publications

Apoptosis of HeLa and CaSki cell lines incubated with All-trans retinoid acid.

Apoptosis of HeLa and CaSki cell lines incubated with All-trans retinoid acid.

Expression of Soluble Vascular Endothelial Growth Factor Receptor-1 in Human Monocyte-Derived Mature Dendritic Cells Contributes to Their Antiangiogenic Property

Inhibitory Effects of Dunning Rat Prostate Tumor Fluid on Proliferation of the Metastatic MAT-LyLu Cell Line

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