Soluble eggshell membrane protein: preparation, characterization and biocompatibility

Feng, Yi; Guo, Zhao‐Xia; Zhang, Lixia; Yu, Jian; Liu, Qiang

doi:10.1016/j.biomaterials.2003.11.052

Cited by 140 publications

(130 citation statements)

References 23 publications

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“…[8,9] SEP/ PEO aqueous blends with concentrations of 3.5-5.0 wt.-% were prepared by adding PEO (900 000 g Á mol À1 ) to a solution of SEP in 0.2% aqueous NaOH.…”

Section: Experimental Partmentioning

confidence: 99%

“…Furthermore, the biocompatibility of SEP was shown to be comparable with collagen type I (the widely used mammalian cell-culture substrate) in a cell culture of NIH3T3. [8,9] Of particular interest to us is the regeneration of the natural fibrous structure of ESM in order to facilitate applications in a biomedical field, such as use as tissue engineering scaffolds and wound dressings, because ESM has shown good biocompatibility and success as wounddressing material. [5] Electrospinning is a recently explored fabrication technique for producing nano to microscale fibers.…”

Section: Introductionmentioning

confidence: 99%

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Mimetics of Eggshell Membrane Protein Fibers by Electrospinning

Feng

Guo

et al. 2004

Macromol. Rapid Commun.

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Summary: Mimetics of eggshell membrane protein fibers have been obtained with an electrospinning technique based on soluble eggshell membrane protein (SEP) prepared previously. Poly(ethylene oxide), a biocompatible and water soluble polymer, is used to improve the processability of SEP. Blends of SEP/PEO aqueous solutions were electrospun. The diameters of the fibers are 0.3–20 μm depending on the concentration of the solution and the proportion of SEP/PEO. Two “cross‐linking” methods are investigated in order to improve the anti‐water property of the fibers.

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“…[8,9] SEP/ PEO aqueous blends with concentrations of 3.5-5.0 wt.-% were prepared by adding PEO (900 000 g Á mol À1 ) to a solution of SEP in 0.2% aqueous NaOH.…”

Section: Experimental Partmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Mimetics of Eggshell Membrane Protein Fibers by Electrospinning

Feng

Guo

et al. 2004

Macromol. Rapid Commun.

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“…5 The biocompatibility of SEP, as demonstrated by cell culture of NIH3T3, is comparable to collagen type I and superior to raw ESM. 6 The availability of SEP makes immobilization possible.…”

Section: Introductionmentioning

confidence: 99%

Immobilization of soluble eggshell membrane protein on polyethylene film surface: Effect on the culture of NIH3T3 in vitro

Feng

Guo

et al. 2005

J of Applied Polymer Sci

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Polyethylene (PE) film surface is modified by combining plasma treatment and soluble eggshell membrane protein (SEP) immobilization. Contact angle measurements, attenuated total reflectance infrared spectroscopy, and X-ray photoelectron spectroscopy confirm that SEP adheres tightly to the PE film surface. Mouse 3T3 fibroblasts are used as model cells to evaluate the biocompatibility of PE film surfaces before and after modification. Plasma pretreatment can incorporate polar groups onto the surface, benefitting tight immobilization of SEP. The hydrophilicity of the PE film surface modified by combining plasma treatment and SEP immobilization is increased as evidenced by contact angle measurements, and the biocompatibility of the surface is greatly improved as shown by cell culture. The surface of the modified material can endure rinsing with 10% acetic acid (a good solvent of SEP), which would be an advantage for further application as a biomaterial.

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“…We recently reported a convenient procedure for preparing soluble eggshell membrane protein (SEP) which is soluble in common non-toxic solvents such as 10% acetic acid [6,7] . SEP is characterized by a detailed study of composition, thermal and surface properties, and biocompatibility, and compared with natural ESM [7] .…”

Section: Introductionmentioning

confidence: 99%

“…SEP is characterized by a detailed study of composition, thermal and surface properties, and biocompatibility, and compared with natural ESM [7] . However, its antibacterial property and biodegradability were not investigated.…”

Section: Introductionmentioning

confidence: 99%