Soluble CD40-ligand (sCD40L, sCD154) plays an immunosuppressive role via regulatory T cell expansion in HIV infection

Jenabian, Mohammad-Ali; Patel, M.H.; Kema, Ido P.; Vyboh, Kishanda; Kanagaratham, Cynthia; Radzioch, Danuta; Thébault, Paméla; Lapointe, Réjean; Gilmore, Norbert; Ancuța, Petronela; Tremblay, Cécile; Routy, Jean‐Pierre

doi:10.1111/cei.12396

Cited by 63 publications

(72 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Taken together, these data underline the different biological properties of membrane-bound CD40L, as provided by a rVV40L controlled infection, as compared to its soluble form, possibly reflecting a differential cross-linking of CD40 receptor expressed on the surfaces of CD14 + monocytes [24,[26][27][28]. …”

Section: Cd40l-expressing Recombinant Vaccinia Virus Induces the Actimentioning

confidence: 70%

“…In contrast, following 24 h infection with VV-WT or rVV40L, lower, nonsignificant increases of CD80 and PD-L1 levels were detected on treated monocytes. Figure 2B shows data from one representative experiment and summarizes the results obtained in three independent assays.Notably, CD1a induction, suggestive of a dendritic cell differentiation was undetectable in all culture conditions (data not shown).Taken together, these data underline the different biological properties of membrane-bound CD40L, as provided by a rVV40L controlled infection, as compared to its soluble form, possibly reflecting a differential cross-linking of CD40 receptor expressed on the surfaces of CD14 + monocytes [24,[26][27][28]. …”

mentioning

confidence: 64%

“…In the TAA-model (melanoma L27MelanA/Mart-1 [26][27][28][29][30][31][32][33][34][35] ), percentages of antigen-specific CD8 + T cells expanded upon priming with rVV40L or s40L-activated CD14 + monocytes, as evaluated by multimer staining on day 8 (Fig. 4A top), were similar.…”

Section: Rvv40l Promotes Generation Of Antigen-specific T CM From Naïmentioning

confidence: 82%

“…4A top), were similar. However, >45% of L27MelanA/Mart-1 [26][27][28][29][30][31][32][33][34][35] multimer positive CD8 + T cells primed with rVV40L-infected CD14 + monocytes displayed a central memory-like phenotype, whereas APCs treated with s40L recombinant protein promoted the differentiation of a significantly lower fraction of naïve CD8 + T cells into central memory-like cells (31.0%) (Fig. 4A).…”

Section: Rvv40l Promotes Generation Of Antigen-specific T CM From Naïmentioning

confidence: 99%

“…Specific binding was evaluated by flow cytometry (FACScalibur; Becton Dickinson Allschwil, Switzerland). To identify cells bearing antigen-specific TCR, CD8 + T cells were stained with soluble HLA-A0201-peptide, streptavidin R-PE conjugated multimers containing L27Melan-A/MART-1 [26][27][28][29][30][31][32][33][34][35] , vaccinia virus H3L [184][185][186][187][188][189][190][191][192] , HCMVpp65 495-504 , and influenza A MP 58-66 (ProImmune, Oxford, UK). Data were analyzed by FlowJo software (Tree Star, Ashland, OR).…”

Section: Flow Cytometrymentioning

confidence: 99%

See 4 more Smart Citations

CD40 ligand‐expressing recombinant vaccinia virus promotes the generation of CD8⁺ central memory T cells

et al. 2015

View full text Add to dashboard Cite

Central memory CD8 + T cells (T CM ) play key roles in the protective immunity against infectious agents, cancer immunotherapy, and adoptive treatments of malignant and viral diseases. CD8+ T CM cells are characterized by specific phenotypes, homing, and proliferative capacities. However, CD8 + T CM -cell generation is challenging, and usually requires CD4 + CD40L + T-cell "help" during the priming of naïve CD8 + T cells. We have generated a replication incompetent CD40 ligand-expressing recombinant vaccinia virus (rVV40L) to promote the differentiation of human naïve CD8 + T cells into T CM specific for viral and tumor-associated antigens. Soluble CD40 ligand recombinant protein (sCD40L), and vaccinia virus wild-type (VV WT), alone or in combination, were used as controls. Here, we show that, in the absence of CD4 + T cells, a single "in vitro" stimulation of naïve CD8 + T cells by rVV40L-infected nonprofessional CD14 + antigen presenting cells promotes the rapid generation of viral or tumor associated antigen-specific CD8 + T cells displaying T CM phenotypic and functional properties. These observations demonstrate the high ability of rVV40L to fine tune CD8 + mediated immune responses, and strongly support the use of similar reagents for clinical immunization and adoptive immunotherapy purposes.Keywords: Antigen presenting cells r Central memory T cells r CD40-CD40L r CD4 + T cells r CD8 + T cells r Immunological memory r Vaccinia virus Additional supporting information may be found in the online version of this article at the publisher's web-site IntroductionGeneration of immunological memory represents a key tenet of the adaptive immune system. Memory T cells display the exquisite Correspondence: Dr. Paul Zajac e-mail: paul.zajac@usb.ch ability to respond to previously experienced antigens with clonal expansion and with the rapid elicitation of multiple effector function [1]. However, the origin of memory CD8 + T cells is still debated, particularly concerning the relationship between effector and memory cells [2].Current understanding is consistent with the "one cell, multiple fates" model postulating that memory and effector T cells C 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim www.eji-journal.eu Eur. J. Immunol. 2016. 46: 420-431 Immunomodulation 421 may arise from single naïve CD8 + T-cell precursor undergoing asymmetric division originating two daughter cells with different cell fates [3]. Alternatively, a linear differentiation model proposes that memory CD8 + T lymphocytes may derive from effector cells surviving the contraction phase of the primary immune response [4]. Memory compartment encompasses lymphocyte subpopulations characterized by different phenotypes, functional properties, and anatomic locations. Two main subsets, central (T CM ) and effector memory (T EM ) lymphocytes, displaying a differential expression of homing and chemokine receptors have consistently been identified [5,6].Notably, T CM are characterized by high proliferative potential in response to antigen stimulation and...

show abstract

Section: Cd40l-expressing Recombinant Vaccinia Virus Induces the Actimentioning

confidence: 70%

mentioning

confidence: 64%

Section: Rvv40l Promotes Generation Of Antigen-specific T CM From Naïmentioning

confidence: 82%

Section: Rvv40l Promotes Generation Of Antigen-specific T CM From Naïmentioning

confidence: 99%

Section: Flow Cytometrymentioning

confidence: 99%

See 3 more Smart Citations

CD40 ligand‐expressing recombinant vaccinia virus promotes the generation of CD8⁺ central memory T cells

et al. 2015

View full text Add to dashboard Cite

show abstract

Soluble immune checkpoint molecules: Serum markers for cancer diagnosis and prognosis

2019

View full text Add to dashboard Cite

Background With the recent advances in the understanding of the interaction of the immune system with developing tumor, it has become imperative to consider the immunological parameters for both cancer diagnosis and disease prognosis. Additionally, in the era of emerging immunotherapeutic strategies in cancer, it is very important to follow the treatment outcome and also to predict the correct immunotherapeutic strategy in individual patients. There being enormous heterogeneity among tumors at different sites or between primary and metastatic tumors in the same individual, or interpatient heterogeneity, it is very important to study the tumor‐immune interaction in the tumor microenvironment and beyond. Importantly, molecular tools and markers identified for such studies must be suitable for monitoring in a noninvasive manner. Recent findings Recent studies have shown that the immune checkpoint molecules play a key role in the development and progression of tumors. In‐depth studies of these molecules have led to the development of most of the cancer immunotherapeutic reagents that are currently either in clinical use or under different phases of clinical trials. Interestingly, many of these cell surface molecules undergo alternative splicing to produce soluble isoforms, which can be tracked in the serum of patients. Conclusions Several studies demonstrate that the serum levels of these soluble isoforms could be used as noninvasive markers for cancer diagnosis and disease prognosis or to predict patient response to specific therapeutic strategies.

show abstract

Inflammatory marker trajectories associated with frailty and ageing in a 20‐year longitudinal study

et al. 2022

View full text Add to dashboard Cite

Objective The aim of this exploratory study was to investigate the development of low‐grade inflammation during ageing and its relationship with frailty. Methods The trajectories of 18 inflammatory markers measured in blood samples, collected at 5‐year intervals over a period of 20 years from 144 individuals aged 65–75 years at the study endpoint, were related to the degree of frailty later in life. Results IFN‐γ‐related markers and platelet activation markers were found to change in synchrony. Chronically elevated levels of IL‐6 pathway markers, such as CRP and sIL‐6R, were associated with more frailty, poorer lung function and reduced physical strength. Being overweight was a possible driver of these associations. More and stronger associations were detected in women, such as a relation between increasing sCD14 levels and frailty, indicating a possible role for monocyte overactivation. Multivariate prediction of frailty confirmed the main results, but predictive accuracy was low. Conclusion In summary, we documented temporal changes in and between inflammatory markers in an ageing population over a period of 20 years, and related these to clinically relevant health outcomes.

show abstract

Soluble CD40-ligand (sCD40L, sCD154) plays an immunosuppressive role via regulatory T cell expansion in HIV infection

Cited by 63 publications

References 53 publications

CD40 ligand‐expressing recombinant vaccinia virus promotes the generation of CD8⁺ central memory T cells

CD40 ligand‐expressing recombinant vaccinia virus promotes the generation of CD8⁺ central memory T cells

Soluble immune checkpoint molecules: Serum markers for cancer diagnosis and prognosis

Inflammatory marker trajectories associated with frailty and ageing in a 20‐year longitudinal study

Contact Info

Product

Resources

About

Soluble CD40-ligand (sCD40L, sCD154) plays an immunosuppressive role via regulatory T cell expansion in HIV infection

Cited by 63 publications

References 53 publications

CD40 ligand‐expressing recombinant vaccinia virus promotes the generation of CD8+ central memory T cells

CD40 ligand‐expressing recombinant vaccinia virus promotes the generation of CD8+ central memory T cells

Soluble immune checkpoint molecules: Serum markers for cancer diagnosis and prognosis

Inflammatory marker trajectories associated with frailty and ageing in a 20‐year longitudinal study

Contact Info

Product

Resources

About

CD40 ligand‐expressing recombinant vaccinia virus promotes the generation of CD8⁺ central memory T cells

CD40 ligand‐expressing recombinant vaccinia virus promotes the generation of CD8⁺ central memory T cells