Soluble arylsulfatases of human brain and some characteristics of the brain-specific arylsulfatase Bm

Lakshmi, Sreeja; Balasubramanian, A.S.

doi:10.1016/0005-2744(80)90234-x

Cited by 13 publications

(7 citation statements)

References 21 publications

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“…Alternatively, contaminating sulfatases have first to be removed prior to using the synthetic substrates under optimized conditions (296). However, some tissues may contain enzyme forms not so different in isoelectric points from arylsulfatase A, which would be lost by the usual ion exchange chromatographies for removal of arylsulfatase A (321,322). It should further be noted that the enzyme reaction may be linear with time for only 10 min (323).…”

Section: The Reaction Catalyzed N-acetylgalactosamine-4-sulfatementioning

confidence: 99%

Glycosaminoglycan Degradation

Kresse¹,

GlüSSL²

1987

Advances in Enzymology - And Related Areas of Molecular Biology

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Section: The Reaction Catalyzed N-acetylgalactosamine-4-sulfatementioning

confidence: 99%

Glycosaminoglycan Degradation

Kresse¹,

GlüSSL²

1987

Advances in Enzymology - And Related Areas of Molecular Biology

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“…2c). In the control sample which was incubated without alkaline phosphatase, about 11% was unbound to DEAE-cellulose presumably due to the ac tion of endogenous phosphatase present in the enzyme preparation [14]. Rat or the chicken anionic B was unaffected by phos phatase treatment ( fig.…”

Section: Alkaline Phosphatase and Neuraminidase Treatment O F The Animentioning

confidence: 99%

“…DEAE-cellulose chromatography using 20 mM Tris-HCl buffer, pH 7.4, has been found to be optimal for the separation of aryl sulfatases A, B and anionic B from the hu man brain on the basis of gel electrophoretic pattern of the isolated enzymes [14,18]. Fig ure 1 shows the arylsulfatases separated from the brains of man, monkey, rabbit, rat and chicken.…”

Section: Separation O F the Brain Arylsulfatases By Deae-cellulose Chmentioning

confidence: 99%

“…The use of DEAE-cellulose and gradient elution with sodium chloride has been found useful in separating and identifying arylsulfatases A, B and anionic B [14,18]. In the pres ent work, we have estimated arylsulfatases A, B and anionic B using DEAE-cellulose chromatography in the brains of primates and nonprimates.…”

Section: Introductionmentioning

confidence: 99%

“…The nature of the a and ¡3 forms of arylsulfatase B of ox liver separated by a pH gradient on CM-Sephadex is not known, although differences in their carbohydrate content have been indicated [7]. In the human brain, a minor anionic form of arylsulfatase B has been identified using DEAE-celluIose chromatography [14,18].…”

Section: Introductionmentioning

confidence: 99%

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Anionic Forms of Brain Arylsulfatase B: Evidence for a Phosphorylated Form in Man and Monkey

Mathew¹,

Balasubramanian²

1983

Dev Neurosci

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Arylsulfatase A, B and an anionic form of B were separated by DEAE-cellulose column chromatography from the brains of man, monkey, rabbit, rat and chicken. The relative proportion of brain arylsulfatases differed from one species to the other. The anionic form of arylsulfatase B was a minor component as compared to arylsulfatase A or B in human and monkey brains while it was a major component in rat and chicken brains. Anionic arylsulfatase B was found in fetal human brains and in newborn monkey brain. In the rat brain, the activities of arylsulfatases A and anionic B showed an increasing trend during development, reaching a peak around 20 days after birth, without any change in their proportions. Treatment with Escherichia coli alkaline phosphatase resulted in the conversion of a major portion (about 70%) of the anionic arylsulfatase B of human and monkey brains into a less charged form which remained unbound to DEAE-cellulose. This conversion by phosphatase was inhibited by inorganic phosphate. Rat and chicken brain anionic arylsulfatase B was not susceptible to alkaline phosphatase. Vibrio cholerae neuraminidase treatment did not significantly affect the charge on anionic arylsulfatase B from any of the species. The results suggested a phosphorylated form of anionic arylsulfatase B exclusively in the primate brain.

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The minor anionic form of arylsulphatase B (arylsulphatase Bm) of monkey brain. Purification and phosphoprotein nature

Lakshmi

Balasubramanian

1984

J. Biosci.

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Soluble arylsulfatases of human brain and some characteristics of the brain-specific arylsulfatase Bm

Cited by 13 publications

References 21 publications

Glycosaminoglycan Degradation

Glycosaminoglycan Degradation

Anionic Forms of Brain Arylsulfatase B: Evidence for a Phosphorylated Form in Man and Monkey

The minor anionic form of arylsulphatase B (arylsulphatase Bm) of monkey brain. Purification and phosphoprotein nature

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