Soluble Adenylyl Cyclase Antibody Profile as a Diagnostic Adjunct in the Assessment of Pigmented Lesions

Magro, Cynthia M.; Crowson, A. Neil; Desman, Garrett; Zippin, Jonathan H.

doi:10.1001/archdermatol.2011.338

Cited by 35 publications

(46 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Mouse anti-sAC monoclonal antibody (R21-IHC, CEP Biotech, Inc., Tamarac, FL) was used at a dilution of 1:750 as described previously (17,18), followed by treatment in a post-primary alkaline phosphatase step for 20 min for signal amplification, application of 3,3Ј-diaminobenzidine for 10 min, and finally washing and mounting with a coverslip.…”

Section: Methodsmentioning

confidence: 99%

“…Aside from its cytosolic localization, sAC is also present in the nucleus, where it controls the activity of the nuclear cAMP response element-binding protein transcription factor through PKA-dependent phosphorylation (12). Recent studies have also demonstrated that sAC migrates from the cytosol to the nucleus when certain cells (keratinocytes and melanocytes) transition from benign cells into cancers, such as squamous cell carcinoma of the skin and melanoma (17,18). These observations suggest that sAC may play a dynamic role in the transition from benign growth to cancer.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Type 10 Soluble Adenylyl Cyclase Is Overexpressed in Prostate Carcinoma and Controls Proliferation of Prostate Cancer Cells

Flacke

Appukuttan

et al. 2013

Journal of Biological Chemistry

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Type 10 Soluble Adenylyl Cyclase Is Overexpressed in Prostate Carcinoma and Controls Proliferation of Prostate Cancer Cells

Flacke

Appukuttan

et al. 2013

Journal of Biological Chemistry

View full text Add to dashboard Cite

“…Moreover, nuclear sAC has been suggested to determine the aggressiveness of certain skin and prostate cancers (Flacke et al, 2013;Magro et al, 2012a;Magro et al, 2012b;Zippin et al, 2010). In the heart, sAC controls mitochondria-dependent apoptosis in both coronary endothelial cells (Kumar et al, 2009) and cardiomyocytes (Appukuttan et al, 2012) (reviewed in Chen et al, 2012b).…”

Section: Reviewmentioning

confidence: 99%

Established and potential physiological roles of bicarbonate-sensing soluble adenylyl cyclase (sAC) in aquatic animals

Tresguerres

Barott

Barron

et al. 2014

Journal of Experimental Biology

View full text Add to dashboard Cite

Soluble adenylyl cyclase (sAC) is a recently recognized source of the signaling molecule cyclic AMP (cAMP) that is genetically and biochemically distinct from the classic G-protein-regulated transmembrane adenylyl cyclases (tmACs). Mammalian sAC is distributed throughout the cytoplasm and it may be present in the nucleus and inside mitochondria. sAC activity is directly stimulated by HCO 3 -, and sAC has been confirmed to be a HCO 3 -sensor in a variety of mammalian cell types. In addition, sAC can functionally associate with carbonic anhydrases to act as a de facto sensor of pH and CO 2 . The two catalytic domains of sAC are related to HCO 3 --regulated adenylyl cyclases from cyanobacteria, suggesting the cAMP pathway is an evolutionarily conserved mechanism for sensing CO 2 levels and/or acid/base conditions. Reports of sAC in aquatic animals are still limited but are rapidly accumulating. In shark gills, sAC senses blood alkalosis and triggers compensatory H + absorption. In the intestine of bony fishes, sAC modulates NaCl and water absorption. And in sea urchin sperm, sAC may participate in the initiation of flagellar movement and in the acrosome reaction. Bioinformatics and RT-PCR results reveal that sAC orthologs are present in most animal phyla. This review summarizes the current knowledge on the physiological roles of sAC in aquatic animals and suggests additional functions in which sAC may be involved.KEY WORDS: V-ATPase, Acid/base, cAMP, Carbonic anhydrase, pH sensing, Proton pump IntroductionCyclic adenosine monophosphate (cAMP) is the signaling molecule of one of the most versatile and evolutionarily conserved signaling pathways. cAMP is produced by adenylyl cyclase enzymes that use ATP as substrate. There are six different classes of adenylyl cyclases distributed throughout Bacteria, Archaea and Eukarya; these classes are unrelated in sequence and structure but all produce cAMP as a result of convergent evolution (Linder and Schultz, 2008). All known eukaryotic adenylyl cyclases, including soluble adenylyl cyclase (sAC) and transmembrane adenylyl cyclase (tmAC) from animals, belong to Class III. Discovery of mammalian sACUntil recently, vertebrate animals were believed to have only one type of adenylyl cyclase, a family of hormone and G-proteinregulated tmACs. Mammals have nine tmAC genes (ADCY1-9), which differ in their tissue and developmental expression as well as in some of their regulatory properties (Cooper, 2003;Hanoune and Defer, 2001 (Braun, 1974;Braun, 1975;Braun, 1990;Braun, 1991;Braun and Dods, 1975). This enzyme was termed 'soluble adenylyl cyclase' (sAC) because its activity is preferentially found in the cytosolic fraction, although some activity is also found associated with membranes. Subsequent immunolocalization and biochemical studies found sAC in the cell cytoplasm and in organelles (Zippin et al., 2003;Zippin et al., 2004). The intracellular localization of sAC changed the assumption that cAMP is produced exclusively in the proximity of the cell membrane by tmACs. Pr...

show abstract

“…The antibody to sAC has emerged as another important diagnostic adjunct in the assessment of melanocytic lesions [24]. A discriminatory staining pattern is observed that separates benign melanocytic proliferations from melanoma.…”

Section: Discussionmentioning

confidence: 99%

“…After over 4 years without evidence of local recurrence, our patient was lost to follow-up. Immunohistochemistry was performed using antibodies to soluble adenylyl cyclase (sAC) according to a previously published procedure [24]. In brief, the R21 is a mouse monoclonal antibody directed against amino acids 203-216 of human sAC fl protein.…”

Section: Case Reportmentioning

confidence: 99%

Oral Atypical Cellular Blue Nevus: An Infiltrative Melanocytic Proliferation

Shumway

Rawal

Allen

et al. 2012

Head and Neck Pathol

Self Cite

View full text Add to dashboard Cite

show abstract

Soluble Adenylyl Cyclase Antibody Profile as a Diagnostic Adjunct in the Assessment of Pigmented Lesions

Cited by 35 publications

References 31 publications

Type 10 Soluble Adenylyl Cyclase Is Overexpressed in Prostate Carcinoma and Controls Proliferation of Prostate Cancer Cells

Type 10 Soluble Adenylyl Cyclase Is Overexpressed in Prostate Carcinoma and Controls Proliferation of Prostate Cancer Cells

Established and potential physiological roles of bicarbonate-sensing soluble adenylyl cyclase (sAC) in aquatic animals

Oral Atypical Cellular Blue Nevus: An Infiltrative Melanocytic Proliferation

Contact Info

Product

Resources

About