Solubilized eggshell membrane supplies a type III collagen-rich elastic dermal papilla

Ohto-Fujita, Eri; Shimizu, Miho; Sano, Shoei; Kurimoto, Masashi; Yamazawa, Kai; Atomi, Tomoaki; Sakurai, Takashi; Murakami, Yoshihiko; Takami, Taku; Murakami, Tomoaki; Hasebe, Yukio; Atomi, Yoriko

doi:10.1007/s00441-018-2954-3

Cited by 25 publications

(22 citation statements)

References 52 publications

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“…1i) were not significantly different. In response to ESM, IMR-90 cells showed significantly higher expression of decorin, type III collagen, and MMP2 compared to the cells grown without ESM, similar to dermal fibroblasts as reported in previous studies (Ohto-Fujita et al 2011;Ohto-Fujita et al 2019).…”

Section: Human Lung Fibroblasts Attached To Esms Induce Type III Collsupporting

confidence: 87%

“…For gene expression analysis in skin layer, nine mice were then divided into two experimental groups, with the respective amounts of ESM in each group being 0 mg (control group, n = 3), 11.0 mg/kg (ESM group, 6 tablets equivalent, n = 6)). Skin layer analysis method was previously described (Ohto-Fujita et al 2019). The primer sequences used in this study were described in Online resource 1.…”

Section: Gene Expression Analysis In Animal Lungs and Skin After Ingementioning

confidence: 99%

“…Additionally, application of SESM to human skin induces an anti-aging effect by increasing arm-skin elasticity and improving wrinkles at the corners of the eyes (Ohto-Fujita et al 2019). Therefore, in the present study, we investigated whether ingestion of ESM orally improves skin and lung within the range of homeostasis in mouse models and human subjects.…”

Section: Introductionmentioning

confidence: 98%

“…We have previously reported that solubilized ESM (SESM) improves the extracellular environment by inducing the expression of decorin, type III collagen, and matrix metalloproteinase-2 (MMP2) on SESM-specific culture surfaces (Ohto-Fujita, et al, 2011) and in the papillary layer of SESM-applied mouse skin (Ohto-Fujita, et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Eggshell membrane promotes homeostasis of elastic skin and lung tissue associated with type III collagen and decorin expression and ameliorates pulmonary fibrosis in a bleomycin mouse model

Ohto-Fujita

Shimizu

Atomi

et al. 2021

Preprint

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The skin and lungs are barriers to environmental threats such as toxic chemicals and microbial pathogens. The integrity of the extracellular matrix (ECM) in the dermal papillae in the skin and the interstitium in the lungs is critical for tissue homeostasis. However, it is difficult to improve the ECM integrity in the skin and lung simultaneously. Previously, we reported that eggshell membrane (ESM) provided a young ECM environment to dermal fibroblasts in vitro and in mouse skin and increased the elasticity of human skin. Herein, lung fibroblasts cultured on ESM showed markedly higher type III collagen, decorin, and MMP2 levels. Oral ESM administration in mice markedly increased the type III collagen and decorin levels in lung tissues after 2 weeks, and type III collagen, decorin, and MMP2 levels in the papillary dermis after 4 weeks. Furthermore, in a double-blind study involving 30 adults, the arm skin elasticity significantly increased after 8 weeks of ESM administration. Simultaneously, the Tiffeneau-Pinelli index, which is correlated with lung elasticity, increased also significantly. To further explore the effects of ESM on the lungs, we used a mouse model of bleomycin-induced fibrosis. In these mice, ESM significantly suppressed fibrosis at 2 weeks and increased the type III collagen levels in the bronchioles and decorin levels in the alveoli, which was implicated in the suppression of lung fibrosis. Thus, oral ESM intake may prevent the age-dependent decline of the papillary dermis and pulmonary fibrosis by improving the extracellular environment in skin and lung tissues.

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Section: Human Lung Fibroblasts Attached To Esms Induce Type III Collsupporting

confidence: 87%

Section: Gene Expression Analysis In Animal Lungs and Skin After Ingementioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Eggshell membrane promotes homeostasis of elastic skin and lung tissue associated with type III collagen and decorin expression and ameliorates pulmonary fibrosis in a bleomycin mouse model

Ohto-Fujita

Shimizu

Atomi

et al. 2021

Preprint

Self Cite

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“…Collagen in wound healing forms a mesh-like scaffold in the damaged tissue and seals off the wound by forming a scar [18][19][20]. In fetal skin the extracellular matrix composition is different as compared to the adult skin, such as the collagen I to collagen III ratio [21,22]. Elastin is not present in the dermis of the fetal skin.…”

Section: Introductionmentioning

confidence: 99%

High resolution ultrasound imaging for repeated measure of wound tissue morphometry, biomechanics and hemodynamics under fetal, adult and diabetic conditions

et al. 2020

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Non-invasive, repeated interrogation of the same wound is necessary to understand the tissue repair continuum. In this work, we sought to test the significance of non-invasive high-frequency high-resolution ultrasound technology for such interrogation. High-frequency high-resolution ultrasound imaging was employed to investigate wound healing under fetal and adult conditions. Quantitative tissue cellularity and elastic strain was obtained for visualization of unresolved inflammation using Vevo strain software. Hemodynamic properties of the blood flow in the artery supplying the wound-site were studied using color Doppler flow imaging. Non-invasive monitoring of fetal and adult wound healing provided unprecedented biomechanical and functional insight. Fetal wounds showed highly accelerated closure with transient perturbation of wound tissue cellularity. Fetal hemodynamics was unique in that sharp fall in arterial pulse pressure (APP) which was rapidly restored within 48h post-wounding. In adults, APP transiently increased post-wounding before returning to the pre-wounding levels by d10 post-wounding. The pattern of change in the elasticity of wound-edge tissue of diabetics was strikingly different. Severe strain acquired during the early inflammatory phase persisted with a slower recovery of elasticity compared to that of the non-diabetic group. Wound bed of adult diabetic mice (db/db) showed persistent hypercellularity compared to littermate controls (db/+) indicative of prolonged inflammation. Normal skin strain of db/+ and db/db were asynchronous. In db/db, severe strain acquired during the early inflammatory phase persisted with a slower recovery of elasticity compared to that of non-diabetics. This study showcases a versatile clinically relevant imaging platform suitable for real-time analyses of functional wound healing.

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Practical Life Science to Understand and Utilize Your Own Body in which Cells Dynamically Respond to Dynamics

Atomi

2022

Emotional Engineering, Vol. 9

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Solubilized eggshell membrane supplies a type III collagen-rich elastic dermal papilla

Cited by 25 publications

References 52 publications

Eggshell membrane promotes homeostasis of elastic skin and lung tissue associated with type III collagen and decorin expression and ameliorates pulmonary fibrosis in a bleomycin mouse model

Eggshell membrane promotes homeostasis of elastic skin and lung tissue associated with type III collagen and decorin expression and ameliorates pulmonary fibrosis in a bleomycin mouse model

High resolution ultrasound imaging for repeated measure of wound tissue morphometry, biomechanics and hemodynamics under fetal, adult and diabetic conditions

Practical Life Science to Understand and Utilize Your Own Body in which Cells Dynamically Respond to Dynamics

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