Solubilization of Phospholipid Bilayers by Surfactants Belonging to the Triton X Series: Effect of Polar Group Size

Partearroyo, M.Aránzazu; Alonso, Alicia; Goñi, Félix M.; Tribout, Marianne; Paredes, Sergio

doi:10.1006/jcis.1996.0103

Cited by 84 publications

(67 citation statements)

References 10 publications

(14 reference statements)

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“…The values extracted are shown in the table in Fig. 6b, and the values agree with results previously obtained from other techniques (de la Maza and Parra 1994;Partearroyo et al 1996;Heerklotz 2002;Sudbrack et al 2011). From a simple partition model, in which R e sat = K.Dw (Heerklotz and Seelig 2000), and assuming that the concentration of free TX-100 at the onset of solubilization is Dw = 0.4 mM, the binding constant K could be obtained for POPC and POPC/chol, as listed in Fig.…”

Section: Solubilization Of Binary Lipid Mixtures Containing Cholesterolsupporting

confidence: 84%

“…In 1985, Lichtenberg proposed a quantitative approach to analyze the lipid-bilayer disruption induced by detergents (Lichtenberg 1985). The approach was further employed to describe detergent-induced hemolysis by the group of Goñi (Partearroyo et al 1992(Partearroyo et al , 1996Requero et al 1995) and ours Preté et al 2002a, b;Domingues et al 2008).…”

Section: Isosmotic Hemolysis and Parameters To Describe Detergent Solmentioning

confidence: 99%

“…Lipid vesicles with tuned lipid composition provide a very robust biomimetic model that allows the understanding of the mechanistic details of intricate processes involving biological membranes. In particular, the solubilization of lipid vesicles by detergents has been extensively studied in recent decades (Partearroyo et al 1996;Patra et al 1998;Heerklotz and Seelig 2000;Sot et al 2002;Heerklotz 2008;Ahyayauch et al 2010;Lichtenberg et al 2013), mainly using large unilamellar vesicles (LUVs). Among the detergents studied, TX-100 has attracted special attention because of its ubiquitous use in solubilization protocols (Hjelmeland 1990).…”

Section: Drms-associated Proteinsmentioning

confidence: 99%

“…To correlate the extent of permeabilization with the process of solubilization, the light scattering of the same samples was measured after the fluorescence intensity kinetic runs. Sample turbidity or light scattering are among the most used methods to assess the solubilization profile of lipid vesicles by detergents, because turbidity/light scattering decreases as lipid vesicles get solubilized into micellar structures (de la Maza and Parra 1994;Partearroyo et al 1996;Lichtenberg et al 2013). Figure 7a shows the results of the permeabilization (red circles) and solubilization (black squares) profiles for the different compositions.…”

Section: Solubilization Of Binary Lipid Mixtures Containing Cholesterolmentioning

confidence: 99%

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Biophysical approaches in the study of biomembrane solubilization: quantitative assessment and the role of lateral inhomogeneity

et al. 2017

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Detergents are amphiphilic molecules widely used to solubilize biological membranes and/or extract their components. Nevertheless, because of the complex composition of biomembranes, their solubilization by detergents has not been systematically studied. In this review, we address the solubilization of erythrocytes, which provide a relatively simple, robust and easy to handle biomembrane, and of biomimetic models, to stress the role of the lipid composition on the solubilization process. First, results of a systematic study on the solubilization of human erythrocyte membranes by different series of non-ionic (Triton, CxEy, Brij, Renex, Tween), anionic (bile salts) and zwitterionic (ASB, CHAPS) detergents are shown. Such quantitative approach allowed us to propose R e sat -the effective detergent/lipid molar ratio in the membrane for the onset of hemolysis as a new parameter to classify the solubilization efficiency of detergents. Second, detergent-resistant membranes (DRMs) obtained as a result of the partial solubilization of erythrocytes by TX-100, C 12 E 8 and Brij detergents are examined. DRMs were characterized by their cholesterol, sphingolipid and specific proteins content, as well as lipid packing. Finally, lipid bilayers of tuned lipid composition forming liposomes were used to investigate the solubilization process of membranes of different compositions/phases induced by Triton X-100. Optical microscopy of giant unilamellar vesicles revealed that pure phospholipid membranes are fully solubilized, whereas the presence of cholesterol renders the mixture partially or even fully insoluble, depending on the composition. Additionally, Triton X-100 induced phase separation in raft-like mixtures, and selective solubilization of the fluid phase only.

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Section: Solubilization Of Binary Lipid Mixtures Containing Cholesterolsupporting

confidence: 84%

Section: Isosmotic Hemolysis and Parameters To Describe Detergent Solmentioning

confidence: 99%

Section: Drms-associated Proteinsmentioning

confidence: 99%

Section: Solubilization Of Binary Lipid Mixtures Containing Cholesterolmentioning

confidence: 99%

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Biophysical approaches in the study of biomembrane solubilization: quantitative assessment and the role of lateral inhomogeneity

et al. 2017

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“…The interaction between phospholipid vesicle membrane and surfactant is of great interest from the standpoints of the solubilization and the reconstitution of membrane proteins, and hence, many researchers have reviewed about it [1][2][3][4][5][6] . In general, the nonionic surfactants have been widely used for this purpose [7][8][9][10][11][12][13][14][15][16][17] . On the other hand, anionic surfactants are used as detergents, and then the interaction of anionic surfactants with phospholipid vesicles might be a good research model for the influence of detergent on skin [18][19][20][21] .…”

Section: Introductionmentioning

confidence: 99%

Effect of Functional Groups on Incorporation Behavior of Amino Acid-Type Surfactant into Phospholipid Vesicle Membrane

et al. 2009

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The incorporation behaviors of some N-dodecanoyl amino acid-type surfactants into L-adipalmitoylphosphatidylcholine (DPPC) vesicles in aqueous solution were investigated. From the leakage measurements of a vesicle-entrapped fluorescence probe, it was found that these surfactants did not affect the DPPC vesicle so much at a very low concentration less than a one-tenth of the critical micelle concentration (CMC), but caused a significant release of the probe form the vesicles even at just below the CMC. The leakage induced by amino acid-type surfactants was promoted by increasing hydrophobicity of the amino acid. However the polarization of DPH embedded in the DPPC membrane was almost unchanged by incorporation of the surfactants at the concentration below the CMC except for the Ndodecanoylphenylalanine system. The binding enthalpy and the binding constant of the surfactant to DPPC vesicles were estimated by isothermal titration calorimetry. While the binding enthalpy was independent of the kind of surfactant, the binding constant increases with increasing hydrophobicity of the amino acid species in the surfactants. The molar ratio of bound surfactant to DPPC molecule in the saturated state was much greater than that for sodium dodecyl sulfate system. Further the ratio was not influenced by the structure of the amino acid side chain.

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Transmission electron microscopy and light scattering studies on the interaction of a nonionic/anionic surfactant mixture with phosphatidylcholine liposomes

Maza

Coderch

López

et al. 1998

Microsc. Res. Tech.

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Solubilization of Phospholipid Bilayers by Surfactants Belonging to the Triton X Series: Effect of Polar Group Size

Cited by 84 publications

References 10 publications

Biophysical approaches in the study of biomembrane solubilization: quantitative assessment and the role of lateral inhomogeneity

Biophysical approaches in the study of biomembrane solubilization: quantitative assessment and the role of lateral inhomogeneity

Effect of Functional Groups on Incorporation Behavior of Amino Acid-Type Surfactant into Phospholipid Vesicle Membrane

Transmission electron microscopy and light scattering studies on the interaction of a nonionic/anionic surfactant mixture with phosphatidylcholine liposomes

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