Solubilization kinetics determines the pulsatory dynamics of lipid vesicles exposed to surfactant

Chabanon, Morgan; Rangamani, Padmini

doi:10.1016/j.bbamem.2018.03.016

Cited by 13 publications

(11 citation statements)

References 65 publications

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“…Strikingly, the vesicle radii exhibited an overall decrease, marked by pulsatile behavior of progressive swelling and abrupt drop for 18:1 GUVs, 18:2 GUVs, and 18:1/18:3 GUVs. This dynamics is similar to those of lipid GUVs undergoing swell-burst cycles induced by osmotic stress (45)(46)(47) or subject to surfactants (48,49). Based on these observations, we propose the following mechanism for the GUVs' dynamics (Fig.…”

Section: Pulsatile Dynamics Of Photooxidized Vesiclessupporting

confidence: 66%

Lipid Unsaturation Properties Govern the Sensitivity of Membranes to Photoinduced Oxidative Stress

Bour

Kruglik

Chabanon

et al. 2019

Biophysical Journal

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Unsaturated lipid oxidation is a fundamental process involved in different aspects of cellular bioenergetics; dysregulation of lipid oxidation is often associated with cell aging and death. To study how lipid oxidation affects membrane biophysics, we used a chlorin photosensitizer to oxidize vesicles of various lipid compositions and degrees of unsaturation in a controlled manner. We observed different shape transitions that can be interpreted as an increase in the area of the targeted membrane followed by a decrease. These area modifications induced by the chemical modification of the membrane upon oxidation were followed in situ by Raman tweezers microspectroscopy. We found that the membrane area increase corresponds to the lipids' peroxidation and is initiated by the delocalization of the targeted double bonds in the tails of the lipids. The subsequent decrease of membrane area can be explained by the formation of cleaved secondary products. As a result of these area changes, we observe vesicle permeabilization after a time lag that is characterized in relation with the level of unsaturation. The evolution of photosensitized vesicle radius was measured and yields an estimation of the mechanical changes of the membrane over oxidation time. The membrane is both weakened and permeabilized by the oxidation. Interestingly, the effect of unsaturation level on the dynamics of vesicles undergoing photooxidation is not trivial and thus carefully discussed. Our findings shed light on the fundamental dynamic mechanisms underlying the oxidation of lipid membranes and highlight the role of unsaturations on their physical and chemical properties.

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Section: Pulsatile Dynamics Of Photooxidized Vesiclessupporting

confidence: 66%

Lipid Unsaturation Properties Govern the Sensitivity of Membranes to Photoinduced Oxidative Stress

Bour

Kruglik

Chabanon

et al. 2019

Biophysical Journal

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“…GUVs were observed by bright field optical microscopy and photooxidation was induced by illumination with a 120 W Metal halide lamp with a 405±10 nm bandpass filter. GUVs undergoing swell-burst cycles induced by osmotic stress (45)(46)(47) or subject to surfactants (48,49). Based on these observations, we propose the following mechanism for the GUVs dynamics ( Fig.…”

Section: Resultsmentioning

confidence: 77%

Lipid unsaturation properties govern the sensitivity of membranes to photo-induced oxidative stress

Bour¹,

Kruglik²,

Chabanon³

et al. 2018

Preprint

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Unsaturated lipid oxidation is a fundamental process involved in different aspects of cellular bioenergetics; dysregulation of lipid oxidation is often associated with cell aging and death. In order to study how lipid oxidation affects membrane biophysics, we used a chlorin photosensitizer to oxidize vesicles of various lipid compositions and degree of unsaturation in a controlled manner. We observed different shape transitions that can be interpreted as an increase in the area of the targeted membrane followed by a decrease. These area modifications induced by the chemical modification of the membrane upon oxidation, were followed in situ by Raman Tweezers Microspectroscopy (RTM). We found that the membrane area increase corresponds to the lipids peroxidation and is initiated by the delocalization of the targeted double bonds in the tails of the lipids. The subsequent decrease of membrane area can be explained by the formation of cleaved secondary products. As a result of these area changes, we observe vesicle permeabilization after a time lag that is characterized in relation with the level of unsaturation. The evolution of photosensitized vesicle radius was measured and yields an estimation of the mechanical changes of the membrane over oxidation time. The membrane is both weakened and permeabilized by the oxidation. Interestingly, the effect of unsaturation level on the dynamics of vesicles undergoing photooxidation is not trivial and thus carefully discussed. Our findings shed light on the fundamental dynamic mechanisms underlying the oxidation of lipid membranes, and highlight the role of unsaturations on their physical and chemical properties

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“… 17 Thermodynamic approaches, including isothermal titration calorimetry, have also been employed extensively to evaluate the detergent CMC 18 , 19 and to characterize the thermodynamics of the surfactant–membrane interaction. 11 , 20 − 22 However, such approaches rely on ensemble averaging across the entire system and cannot report on individual vesicle conformation.…”

mentioning

confidence: 99%

Tween-20 Induces the Structural Remodeling of Single Lipid Vesicles

Dresser

Graham

Miller

et al. 2022

J. Phys. Chem. Lett.

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The solubilization of lipid membranes by Tween-20 is crucial for a number of biotechnological applications, but the mechanistic details remain elusive. Evidence from ensemble assays supports a solubilization model that encompasses surfactant association with the membrane and the release of mixed micelles to solution, but whether this process also involves intermediate transitions between regimes is unanswered. In search of mechanistic origins, increasing focus is placed on identifying Tween-20 interactions with controllable membrane mimetics. Here, we employed ultrasensitive biosensing approaches, including single-vesicle spectroscopy based on fluorescence and energy transfer from membrane-encapsulated molecules, to interrogate interactions between Tween-20 and submicrometer-sized vesicles below the optical diffraction limit. We discovered that Tween-20, even at concentrations below the critical micellar concentration, triggers stepwise and phase-dependent structural remodeling events, including permeabilization and swelling, in both freely diffusing and surface-tethered vesicles, highlighting the substantial impact the surfactant has on vesicle conformation and stability prior to lysis.

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Solubilization kinetics determines the pulsatory dynamics of lipid vesicles exposed to surfactant

Cited by 13 publications

References 65 publications

Lipid Unsaturation Properties Govern the Sensitivity of Membranes to Photoinduced Oxidative Stress

Lipid Unsaturation Properties Govern the Sensitivity of Membranes to Photoinduced Oxidative Stress

Lipid unsaturation properties govern the sensitivity of membranes to photo-induced oxidative stress

Tween-20 Induces the Structural Remodeling of Single Lipid Vesicles

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