Solubility, phase transition, kinetic ripening and growth rates of porcine pancreatic α-amylase isoenzymes

Boistelle, R.; Astier, Jean-Pierre; Marchis-Mouren, G.; Desseaux, Véronique; Haser, R.

doi:10.1016/0022-0248(92)90015-b

Cited by 60 publications

(62 citation statements)

References 19 publications

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“…When the crystals of -amylase were nucleated from clear solution they were always observed growing at the bottom of the cell with no orientation of the crystals for either orthorhombic polymorphs A or B (Boistelle et al, 1992). This is in contrast to the growth experiments of seed crystals of polymorph A for which crystals are found oriented with their [001] axis perpendicular to the direction of the magnetic ®eld (Fig.…”

Section: Bptimentioning

confidence: 88%

“…The crystals under consideration are different polymorphs of porcine pancreatic -amylase (Boistelle et al, 1992;Veesler et al, 1994), bovine pancreatic trypsin inhibitor (BPTI) and different ®sh parvalbumins (Lafont, 1996). Since we have previously established their phase diagrams and the crystallization conditions, we decided to ®rst carry out experiments with these proteins, together with chicken eggwhite lysozyme which is easily available and also the protein most studied by crystal growers.…”

Section: Proteinsmentioning

confidence: 99%

“…Unlike most proteins -amylase crystallizes without any crystallization agent so that supersaturation may be achieved either by evaporation or, as in our case, by concentrating the sample solution through an Amicon membrane (Centricon-10). For nucleation experiments, we used protein solutions, the concentration of which was 50 mg ml À1 which corresponds to 9 10 ( Boistelle et al, 1992). For the growth experiments of seed crystals the concentration was 40 mg ml À1 which correspond to 9 8.…”

Section: Proteinsmentioning

confidence: 99%

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Protein crystals orientation in a magnetic field

1998

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Nucleation and crystal growth of hen egg-white lysozyme, bovine pancreatic trypsin inhibitor and porcine pancreatic -amylase were carried out in the presence of a magnetic ®eld of 1.25 T produced by small permanent magnets. Crystals were oriented in the magnetic ®eld, except when heterogeneous nucleation occurred. The orientation of protein crystals in the presence of a magnetic ®eld can be attributed to the anisotropic diamagnetic susceptibility of proteins resulting from the large anisotropy of the -helices due to the axial alignment of the peptide bonds.

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Section: Bptimentioning

confidence: 88%

Section: Proteinsmentioning

confidence: 99%

Section: Proteinsmentioning

confidence: 99%

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Protein crystals orientation in a magnetic field

1998

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“…3 Protein phase separations can also be the cause of human diseases, such as cataract 4 or sickle cell disease. 5 Despite their fundamental importance, phase diagrams of only a few proteins have been studied: insulin, 6 fibrinogen, 7 γ-crystallin, 8 lysozyme, 4,9-12 concanavalin, 13 canavalin, 14 R-amylase, 15,16 collagenase, 17 bovine pancreatic trypsin inhibitor (BPTI), 16,18 apoferritin, 19 endoglucanase A, 20 and the photochemical reaction center from Rhodobacter sphaeroides Y. 21 Recently, there has been a renewed enthusiasm for phasetransition studies of colloids 22 and proteins [22][23][24][25][26][27] due to advances in experimental and computational technologies.…”

Section: Introductionmentioning

confidence: 99%

Exploring Bovine Pancreatic Trypsin Inhibitor Phase Transitions

et al. 2006

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This paper presents an investigation of the phase diagram of BPTI (bovine pancreatic trypsin inhibitor)/350 mM KSCN at pH 4.9 by direct observation and numerical simulations. We report optical microscopy and light and X-ray scattering experiments coupled with theoretical data analysis using numerical tools. The phase diagram is thoroughly determined, as a function of temperature. Two polymorphs are observed by video microscopy and their solubility measured. In this phase diagram, the liquid-liquid phase separation (LLPS) is metastable with respect to the solid-liquid phase separation. Above the T L-L boundary curve, solutions are composed of a mixture of BPTI monomers and decamers. Attractive interactions are stronger between decamers than between monomers. Below the T L-L boundary curve, the dense phase is highly concentrated in protein and composed of BPTI decamers alone. Thus, the driving force for liquid-liquid or liquid-solid phase separation is the attraction between decamers at low pH. The structure factors of the dense phases are characteristic of repulsive dense phases because of a hard sphere repulsion core, meaning that in the dense phase proteins are actually in contact (interparticle distance of 53 Å). In agreement with the Oswald rule of stages, LLPS occurs prior to and impedes the solid nucleation.

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“…These may, in turn, be applied under different physical conditions such as temperature, which can be an important variable in biological macromolecule crystallization, since an understanding of the phase diagram is useful for the production of suitable crystals for structural biology (Boistelle et al 1992;Lafont et al 1994Lafont et al , 1997Revalor et al 2010). …”

Section: Effect Of Temperature On the Solubilitymentioning

confidence: 99%

Crystallization, solubility and thermodynamics of the highly thermostable glucose isomerase from Streptomyces sp. strain

Borgi¹,

Rhimi²,

Kadri³

2014

gpb

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Abstract. The crystallization behaviour of the highly thermostable glucose isomerase from the Streptomyces sp. strain isolated from Tunisian soil was investigated using ammonium sulfate as a precipitating agent. We established phase diagrams at different temperatures and protein concentrations. It was found that the solubility increased with increasing temperature and decreased with increasing salt concentration. The temperature-dependent solubility was used to characterize the thermodynamic parameters of crystallization such as enthalpy, entropy and free energy.

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Solubility, phase transition, kinetic ripening and growth rates of porcine pancreatic α-amylase isoenzymes

Cited by 60 publications

References 19 publications

Protein crystals orientation in a magnetic field

Protein crystals orientation in a magnetic field

Exploring Bovine Pancreatic Trypsin Inhibitor Phase Transitions

Crystallization, solubility and thermodynamics of the highly thermostable glucose isomerase from Streptomyces sp. strain

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