Solubility of Uroporphyrin I in Ethyl Acetate

Dresel, E. I. B.; Tooth, B. E.

doi:10.1038/174271a0

Cited by 19 publications

(16 citation statements)

References 5 publications

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“…(26). Uroporphyrin was determined by the method of Dresel and Tooth (27) as outlined by Dresel and Falk (28). On a number of occasions, after the complete extraction of coproand protoporphyrins with 15% HCl, the ethyl acetate layer was tested for the presence of porphyrinogens.…”

Section: Methodsmentioning

confidence: 99%

In Vitro Pyrrole and Porphyrin Synthesis in Lead Poisoning and Iron Deficiency*

Lichtman¹,

Feldman²

1963

J. Clin. Invest.

116

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Section: Methodsmentioning

confidence: 99%

In Vitro Pyrrole and Porphyrin Synthesis in Lead Poisoning and Iron Deficiency*

Lichtman¹,

Feldman²

1963

J. Clin. Invest.

116

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“…The determination of uroporphyrin in biological materials is rendered difficult by its insolubility in the usual organic solvents. Dresel & Tooth (1954) found, however, that uroporphyrins I and III could both be extracted by ethyl acetate from solutions within the narrow pH range 3-0-3'2. Kennedy (1953) noted the extractability of uroporphyrin from acid solutions by cyclohexanone and this observation led to the quantitative technique of Dresel, Rimington & Tooth (1956), which has since become the standard procedure for determination of uroporphyrin in urine (Rimington, 1961).…”

mentioning

confidence: 89%

Partition of porphyrins between cyclohexanone and aqueous sodium acetate as a function of pH. Determination of uroporphyrin and of hydrophilic porphyrin conjugates

Rimington

Benson

1967

Biochemical Journal

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1. The partition of uroporphyrins I and III, coproporphyrins I and III, haematoporphyrin IX, porphyrin c and a hydrophilic porphyrin-peptide fraction from variegate-porphyria faeces has been studied in systems of equal volumes of cyclohexanone and sodium acetate buffers of varying pH and concentration. 2. The concentration of acetate in the aqueous phase has little effect on the partition of porphyrin c, but markedly influences that of uroporphyrin. At 50% acetate saturation and pH4.5, only 5% enters the cyclohexanone phase whereas 60% of porphyrin c is extracted under similar conditions. 3. This circumstance forms the basis of a method for the determination of hydrophilic porphyrin-peptides in variegate-porphyria urine. Its reliability has been checked in model experiments. 4. At pH1.5 and an aqueous phase half-saturated with sodium acetate, an equal volume of cyclohexanone removes 95-97% of uroporphyrin and about 55% of porphyrin c. Uroporphyrin may therefore be determined as a second step in the method. 5. For the routine determination of uroporphyrin in systems free from other hydrophilic porphyrins, cyclohexanone extraction may be performed at any pH in the range 1.0-3.0.

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“…Extraction, fractionation and determination of copro and protoporphyrin followed a modification of the procedures of Schwartz and W i k~f f .~ Uroporphyrin was determined by the method of Dresel and Tooth. 6 The porphyrins reported represent the differences between the control tubes and those with delta aminolevulinic acid.…”

Section: Porphyrin Determinationsmentioning

confidence: 99%