Solubility of Stevioside and Rebaudioside A in water, ethanol and their binary mixtures

Abstract

In order to investigate the solubility of Stevioside and Rebaudioside A in different solvents (ethanol, water, ethanol:water 30:70 and ethanol:water 70:30), supersaturated solutions of pre-crystalized steviol glycosides were maintained at different temperatures (from 5°C to 50°C) to reach equilibrium. Under these conditions significant differences were found in the extent of solubility. Rebaudioside A was poorly soluble in ethanol and water, and Stevioside was poorly soluble in water. Solvent mixtures more effectively promoted solubilisation, and a significant effect of temperature on solubility was observed. The two steviol glycosides showed higher solubilities and this behavior was promoted by the presence of the other sweetener. The polarity indices of the solvents were determined, and helped to explain the observed behavior. Several solute-solvent and solute-solute interactions can occur, along with the incidence of a strong affinity between solvents. The obtained results are in accordance with technological applications of ethanol, water and their binary mixtures for Stevioside and Rebaudioside A separations.

Solid–Liquid Equilibrium of Rebaudioside A in Pure and Binary Mixed Solvents at T = (288.15 to 328.15) K

“…Table S2 shows that our experimental data are in good agreement with the literature values, especially with ref . However, the obtained values for the solubility of rebaudioside A in ethanol in ref are not in agreement with reported data in this work and the values reported in the literature. , These deviations may be caused by differences in purity source of compound, determination method, and the crystal forms of the rebaudioside A.…”

“…The obtained values indicate that the experimental method used in this work is reliable. In this way, in Table S2 in the Supporting Information, the solubility data of rebaudioside A in ethanol at various temperatures in this work have been compared with the values reported in the literature ,, and also shown graphically in Figure S1 in the Supporting Information. Table S2 shows that our experimental data are in good agreement with the literature values, especially with ref .…”

“…Hence, in this work the solubility of rebaudioside A was measured in some pure (methanol, ethanol, 1-propanol, 2-propanol, acetonitrile, 2-methoxyethanol, 2-ethoxyethanol, 2-propoxyethanol, 2-buthoxyethanol, and ethylene glycol) and binary mixed solvents (methanol + ethanol and 2-methoxyethanol + ethanol) by high performance liquid chromatography (HPLC) method at temperatures ranging from (288.15 to 328.15) K and atmospheric pressure. Such information is not available in the literature, and there is solubility data for rebaudioside A in only a few solvents. ,, The modified Apelblat and nonrandom two-liquid (NRTL) models were used to correlate the measured solubility data in all studied solvents.…”

Application of Antiviral, Antioxidant and Antibacterial Glycyrrhiza glabra L., Trifolium pratense L. Extracts and Myristica fragrans Houtt. Essential Oil in Microcapsules

“…Ethanolic Trifolium pratense L. extract had a significantly higher total phenol content compared to aqueous Glycyrrhiza glabra L. extract ( p < 0.05), and the exact correlation was observed comparing total flavonoids. Varying solubility of the phenolic compounds due to different solvents could be explained by the solvent polarity, and ethanol is more efficient in extracting lower molecular weight polyphenols, especially glycosides [ 48 , 49 ].…”

Moisture sorption isotherms of reduced‐sugar confectionery coatings elaborated with stevia: Mathematical modeling and thermodynamic analysis

“…The solution was diluted to obtain the total SG content required for this study. Reverse phase high-performance liquid chromatography was used to obtain the SG profile, performing the protocol published by Celaya et al (2016). The SG were identified by retention time and quantified by measuring individual peaks (Figure 1).…”